Reviewed, UniProtKB/Swiss-Prot Q53932 (IPNS_STRCT)

Names and origin

Protein names

Recommended name:
    Isopenicillin N synthetase
    EC=1.21.3.1
Alternative name(s):
    Isopenicillin N synthase
      Short name=IPNS

Gene names

Name:	pcbC
Synonyms:	ipnS

Organism

Streptomyces cattleya

Taxonomic identifier

29303 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
Catalytic activity	N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW isopenicillin-N synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Isopenicillin N synthetase		PRO_0000219505
Sites
	Metal binding	213	1	Iron By similarity
	Metal binding	215	1	Iron By similarity
	Metal binding	269	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q53932-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BCC0CFFCF7C07AF1	FASTA	321	36,577
	10 20 30 40 50 60 MPVLMPSADV PTIDISPQLF GTDPTPRRTS RGRSTRPARG SGFFYASHHG IDVRRLQTWS 70 80 90 100 110 120 NESTTMTDQR STTWRSTRYN ENNSHVRNGY YMARPGRETV ESWCYLNPSF GEDHPMMKAG 130 140 150 160 170 180 TPMHEVNVWP DEERHPDFGS FGEQYHREVS ASRRCCCGAS RWRRQAGESS SNEVTEEDTL 190 200 210 220 230 240 SAVSMIRYPY LDPYPEAAIK TGPDGTRLSF EDHLDVSMIT VLSKTEVQNL QVETVDGWQS 250 260 270 280 290 300 LPTSGENFLI NCGTYLGYLT NDYFPAPNHR VKYVNAERLS LPFFLHAGQN SVMKPFTRRT 310 320 GDRKLNPAVT YGEYLQEGFT R

References

[1]	"Cloning and sequencing the isopenicillin N synthetase (IPNS) gene from Streptomyces cattleya." Wang Y., Li R. Wei Sheng Wu Xue Bao 36:87-92(1996) [PubMed: 9206271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78166 Genomic DNA. Translation: BAA11234.1.
PIR	A58458.
3D structure databases
HSSP	HSSP built from PDB template 1QJE based on UniProtKB P05326.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.21.3.1. 142837.
Family and domain databases
InterPro	IPR002057. Isopenicillin-N_synth_CS. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS00185. IPNS_1. 1 hit. PS00186. IPNS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPNS_STRCT

Accession

Primary (citable) accession number: Q53932

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)