Q53844 (RNC_SPICI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:

rnc

Organism

Spiroplasma citri

Taxonomic identifier

2133 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Spiroplasmataceae › Spiroplasma

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000180431
Regions
	Domain	15 – 142	128	RNase III
	Domain	169 – 240	72	DRBM
Sites
	Active site	59	1	Potential
	Active site	131	1	By similarity
	Metal binding	55	1	Magnesium By similarity
	Metal binding	128	1	Magnesium By similarity
	Metal binding	131	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q53844 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1A5A02EA1AD95D82	FASTA	248	28,931
	10 20 30 40 50 60 MQFINKQSEQ LFIELKAFFK QYHVFIKERQ YYLEALTHNS YANEHNLSYT YQRMEFLGDA 70 80 90 100 110 120 ILAKEISLYL FLSFPDKNEG EITNLRSKIV REGTLAELVR RMNWAPFLLL GKGEIKTKGY 130 140 150 160 170 180 EKNRILADIY ESMIAALYLD LGEDVVRTFI NNTLIRMVSN PGFFDKIRDY KTELQEFLQA 190 200 210 220 230 240 GDARTLEYKL IKESQPLEGN RVLYTVVAEI GGIRYGEGCG YTHKEAEQLA ARDALQKLAT KSKYHFEK

References

[1]	"Spiroplasma citri virus SpV1: characterization of viral sequences present in the spiroplasmal host chromosome." Bebear C.-M., Aullo P., Bove J.-M., Renaudin J. Curr. Microbiol. 32:134-140(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27556 / NCPPB 2647 / R8A2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28972 Genomic DNA. Translation: AAA84999.1.
3D structure databases
ProteinModelPortal	Q53844.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_SPICI

Accession

Primary (citable) accession number: Q53844

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program