ID   Q53752_KITAU            Unreviewed;       163 AA.
AC   Q53752;
DT   01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 120.
DE   SubName: Full=Ribonuclease {ECO:0000313|EMBL:AAA26806.1};
DE            EC=3.1.4.8 {ECO:0000313|EMBL:AAA26806.1};
DE   Flags: Precursor;
GN   Name=ribonuclease {ECO:0000313|EMBL:AAA26806.1};
GN   ORFNames=HS99_0012425 {ECO:0000313|EMBL:OEV33585.1};
OS   Kitasatospora aureofaciens (Streptomyces aureofaciens).
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Kitasatospora.
OX   NCBI_TaxID=1894 {ECO:0000313|EMBL:AAA26806.1};
RN   [1] {ECO:0000313|EMBL:AAA26806.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=R8/26 {ECO:0000313|EMBL:AAA26806.1};
RA   Proia R.L.;
RL   Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007829|PDB:1PY3, ECO:0007829|PDB:1PYL}
RP   X-RAY CRYSTALLOGRAPHY (1.51 ANGSTROMS) OF 67-163, DISULFIDE BONDS, AND
RP   ACTIVE SITE.
RX   PubMed=15213380; DOI=10.1107/S0907444904009035;
RA   Sevcik J., Dauter Z., Wilson K.S.;
RT   "Crystal structure reveals two alternative conformations in the active site
RT   of ribonuclease Sa2.";
RL   Acta Crystallogr. D 60:1198-1204(2004).
RN   [3] {ECO:0007829|PDB:3D4A, ECO:0007829|PDB:3D5G}
RP   X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 67-163, AND DISULFIDE BONDS.
RX   PubMed=19558492; DOI=10.1111/j.1742-4658.2009.07125.x;
RA   Bauerova-Hlinkova V., Dvorsky R., Perecko D., Povazanec F., Sevcik J.;
RT   "Structure of RNase Sa2 complexes with mononucleotides--new aspects of
RT   catalytic reaction and substrate recognition.";
RL   FEBS J. 276:4156-4168(2009).
RN   [4] {ECO:0000313|EMBL:OEV33585.1, ECO:0000313|Proteomes:UP000037395}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10762 {ECO:0000313|EMBL:OEV33585.1}, and ATCC 10762 / DSM
RC   40127 / CCM 3239 / JCM 4008 / LMG 5968 / NBRC 12843 / NCIMB 8234 /
RC   A-377 {ECO:0000313|Proteomes:UP000037395};
RA   Zhang J.E., Yang H., Guo J., Deng Z., Luo H., Luo M., Zhao B.;
RL   Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|Proteomes:UP000037395}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10762 / DSM 40127 / CCM 3239 / JCM 4008 / LMG 5968 / NBRC
RC   12843 / NCIMB 8234 / A-377 {ECO:0000313|Proteomes:UP000037395};
RA   Gradnigo J.S., Johnson N., Somerville G.A.;
RT   "Sequencing, assembly and comparative genomics of S. aureofaciens ATCC
RT   10762.";
RL   Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000313|EMBL:OEV33585.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10762 {ECO:0000313|EMBL:OEV33585.1};
RA   Gradnigo J.S., Johnson N., Somerville G.A.;
RT   "Sequencing, Assembly and Comparative Genomics of S. aureofaciens ATCC
RT   10762.";
RL   Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the ribonuclease N1/T1 family.
CC       {ECO:0000256|ARBA:ARBA00009006}.
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DR   EMBL; M88615; AAA26806.1; -; Genomic_DNA.
DR   EMBL; JPRF03000065; OEV33585.1; -; Genomic_DNA.
DR   RefSeq; WP_030597940.1; NZ_LBHA01000258.1.
DR   PDB; 1PY3; X-ray; 1.80 A; A/B/C=67-163.
DR   PDB; 1PYL; X-ray; 1.51 A; A/B=67-163.
DR   PDB; 3D4A; X-ray; 2.20 A; A/B/C=67-163.
DR   PDB; 3D5G; X-ray; 1.80 A; A/B/C=67-163.
DR   PDB; 3D5I; X-ray; 2.20 A; A/B/C=67-163.
DR   PDB; 3DGY; X-ray; 1.80 A; A/B/C=67-163.
DR   PDB; 3DH2; X-ray; 2.25 A; A/B/C/D=67-163.
DR   PDBsum; 1PY3; -.
DR   PDBsum; 1PYL; -.
DR   PDBsum; 3D4A; -.
DR   PDBsum; 3D5G; -.
DR   PDBsum; 3D5I; -.
DR   PDBsum; 3DGY; -.
DR   PDBsum; 3DH2; -.
DR   AlphaFoldDB; Q53752; -.
DR   SMR; Q53752; -.
DR   MINT; Q53752; -.
DR   GeneID; 33985910; -.
DR   OrthoDB; 5326845at2; -.
DR   EvolutionaryTrace; Q53752; -.
DR   Proteomes; UP000037395; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004521; F:RNA endonuclease activity; IEA:InterPro.
DR   CDD; cd00607; RNase_Sa; 1.
DR   Gene3D; 3.10.450.30; Microbial ribonucleases; 1.
DR   InterPro; IPR000026; Gua-sp_ribonuclease_Sa-like.
DR   InterPro; IPR016191; Ribonuclease/ribotoxin.
DR   Pfam; PF00545; Ribonuclease; 1.
DR   SUPFAM; SSF53933; Microbial ribonucleases; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:1PY3, ECO:0007829|PDB:1PYL};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:AAA26806.1};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW   Reference proteome {ECO:0000313|Proteomes:UP000037395}.
FT   REGION          35..68
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        122
FT                   /note="EMO_00062 proton shuttle (general acid/base)"
FT                   /evidence="ECO:0007829|PDB:1PYL"
FT   ACT_SITE        152
FT                   /note="EMO_00062 proton shuttle (general acid/base)"
FT                   /evidence="ECO:0007829|PDB:1PYL"
FT   SITE            133
FT                   /note="EMO_00038 activator,EMO_00035 transition state
FT                   stabiliser"
FT                   /evidence="ECO:0007829|PDB:1PYL"
FT   SITE            137
FT                   /note="EMO_00038 activator,EMO_00035 transition state
FT                   stabiliser"
FT                   /evidence="ECO:0007829|PDB:1PYL"
FT   DISULFID        75..163
FT                   /evidence="ECO:0007829|PDB:1PY3, ECO:0007829|PDB:1PYL"
SQ   SEQUENCE   163 AA;  17099 MW;  22DFAB7FE8727F9A CRC64;
     MTRSKSPLVV GAVLILAVLA GVGYLLAGRG GSTHPKAAAS SAAAGTSAPK SSAPKPSPPA
     GGAWTPADPA LADVCRTKLP SQAQDTLALI AKNGPYPYNR DGVVFENRES RLPKKGNGYY
     HEFTVVTPGS NDRGTRRVVT GGYGEQYWSP DHYATFQEID PRC
//