ID   Q53707_STAAU            Unreviewed;       668 AA.
AC   Q53707;
DT   01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT   01-NOV-1996, sequence version 1.
DT   24-JAN-2024, entry version 85.
DE   SubName: Full=MecA PBP2' (penicillin binding protein 2') {ECO:0000313|EMBL:CAA36829.1};
DE   SubName: Full=PBP2A {ECO:0000313|EMBL:CAA74376.1};
GN   Name=mecA {ECO:0000313|EMBL:CAA74376.1};
OS   Staphylococcus aureus.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280 {ECO:0000313|EMBL:CAA36829.1};
RN   [1] {ECO:0000313|EMBL:CAA36829.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=NCTC8325 {ECO:0000313|EMBL:CAA36829.1};
RX   PubMed=2227446; DOI=10.1016/0378-1119(90)90481-6;
RA   Ryffel C., Tesch W., Birch-Machin I., Reynolds P.E., Barberis-Maino L.,
RA   Kayser F.H., Berger-Bachi B.;
RT   "Sequence comparison of mecA genes isolated from methicillin-resistant
RT   Staphylococcus aureus and Staphylococcus epidermidis.";
RL   Gene 94:137-138(1990).
RN   [2] {ECO:0000313|EMBL:CAA74376.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BB270 {ECO:0000313|EMBL:CAA74376.1}, and BMS-1
RC   {ECO:0000313|EMBL:CAA74376.1};
RX   PubMed=9440511;
RA   Wu S., de Lencastre H., Tomasz A.;
RT   "Genetic organization of the mecA region in methicillin-susceptible and
RT   methicillin-resistant strains of Staphylococcus sciuri.";
RL   J. Bacteriol. 180:236-242(1998).
CC   -!- SIMILARITY: Belongs to the transpeptidase family.
CC       {ECO:0000256|ARBA:ARBA00007171}.
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DR   EMBL; X52593; CAA36829.1; -; Genomic_DNA.
DR   EMBL; Y14051; CAA74376.1; -; Genomic_DNA.
DR   PIR; JQ0773; JQ0773.
DR   AlphaFoldDB; Q53707; -.
DR   SMR; Q53707; -.
DR   BindingDB; Q53707; -.
DR   ChEMBL; CHEMBL2362972; -.
DR   ChEMBL; CHEMBL2363021; -.
DR   DrugBank; DB01603; Meticillin.
DR   DrugBank; DB00417; Phenoxymethylpenicillin.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:InterPro.
DR   Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR   Gene3D; 3.90.1310.10; Penicillin-binding protein 2a (Domain 2); 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR007887; MecA_N.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR005311; PBP_dimer.
DR   InterPro; IPR036138; PBP_dimer_sf.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   PANTHER; PTHR30627:SF25; PENICILLIN-BINDING PROTEIN 3; 1.
DR   PANTHER; PTHR30627; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE; 1.
DR   Pfam; PF05223; MecA_N; 1.
DR   Pfam; PF03717; PBP_dimer; 1.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR   SUPFAM; SSF54427; NTF2-like; 1.
DR   SUPFAM; SSF56519; Penicillin binding protein dimerisation domain; 1.
PE   3: Inferred from homology;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        7..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          34..140
FT                   /note="NTF2-like N-terminal transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF05223"
FT   DOMAIN          148..310
FT                   /note="Penicillin-binding protein dimerisation"
FT                   /evidence="ECO:0000259|Pfam:PF03717"
FT   DOMAIN          346..656
FT                   /note="Penicillin-binding protein transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF00905"
SQ   SEQUENCE   668 AA;  76266 MW;  5E58F2572B359EEA CRC64;
     MKKIKIVPLI LIVVVVGFGI YFYASKDKEI NNTIDAIEDK NFKQVYKDSS YISKSDNGEV
     EMTERPIKIY NSLGVKDINI QDRKIKKVSK NKKRVDAQYK IKTNYGNIDR NVQFNFVKED
     GMWKLDWDHS VIIPGMQKDQ SIHIENLKSE RGKILDRNNV ELANTGTHMR LGIVPKNVSK
     KDYKAIAKEL SISEDYINNK WIKIGYKMIP SFHFKTVKKM DEYLSDFAKK FHLTTNETES
     RNYPLEKATS HLLGYVGPIN SEELKQKEYK GYKDDAVIGK KGLEKLYDKK LQHEDGYRVT
     IVDDNSNTIA HTLIEKKKKD GKDIQLTIDA KVQKSIYNNM KNDYGSGTAI HPQTGELLAL
     VSTPSYDVYP FMYGMSNEEY NKLTEDKKEP LLNKFQITTS PGSTQKILTA MIGLNNKTLD
     DKTSYKIDGK GWQKDKSWGG YNVTRYEVVN GNIDLKQAIE SSDNIFFARV ALELGSKKFE
     KGMKKLGVGE DIPSDYPFYN AQISNKNLDN EILLADSGYG QGEILINPVQ ILSIYSALEN
     NGNINAPHLL KDTKNKVWKK NIISKENINL LNDGMQQVVN KTHKEDIYRS YANLIGKSGT
     AELKMKQGES GRQIGWFISY DKDNPNMMMA INVKDVQDKG MASYNAKISG KVYDELYENG
     NKKYDIDE
//