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Protein

Sensor protein LytS

Gene

lytS

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system LytR/LytS that regulates genes involved in autolysis and cell wall metabolism. Regulates the activity of the cell wall-associated murein hydrolase through regulation of lrgA and lrgB.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein LytS (EC:2.7.13.3)
Alternative name(s):
Autolysin sensor kinase
Gene namesi
Name:lytS
Ordered Locus Names:SAOUHSC_00230
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 28HelicalSequence analysisAdd BLAST23
Transmembranei40 – 62HelicalSequence analysisAdd BLAST23
Transmembranei88 – 110HelicalSequence analysisAdd BLAST23
Transmembranei123 – 140HelicalSequence analysisAdd BLAST18
Transmembranei155 – 172HelicalSequence analysisAdd BLAST18
Transmembranei184 – 206HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747971 – 584Sensor protein LytSAdd BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei390Phosphohistidine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00230.

Structurei

3D structure databases

ProteinModelPortaliQ53705.
SMRiQ53705.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini311 – 362GAFAdd BLAST52
Domaini363 – 580Histidine kinaseAdd BLAST218

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 histidine kinase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108HRK. Bacteria.
COG3275. LUCA.
HOGENOMiHOG000242218.
KOiK07704.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR010559. Sig_transdc_His_kin_internal.
IPR011620. Sig_transdc_His_kinase_LytS_TM.
[Graphical view]
PfamiPF07694. 5TM-5TMR_LYT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF06580. His_kinase. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLTMLLLE RVGLIIILAY VLMNIPYFKN LMNRRRTWKA RWQLCIIFSL
60 70 80 90 100
FALMSNLTGI VIDHQHSLSG SVYFRLDDDV SLANTRVLTI GVAGLVGGPF
110 120 130 140 150
VGLFVGVISG IFRVYMGGAD AQVYLISSIF IGIIAGYFGL QAQRRKRYPS
160 170 180 190 200
IAKSAMIGIV MEMIQMLSIL TFSHDKAYAV DLISLIALPM IIVNSVGTAI
210 220 230 240 250
FMSIIISTLK QEEQMKAVQT HDVLQLMNQT LPYFKEGLNR ESAQQIAMII
260 270 280 290 300
KNLMKVSAVA ITSKNEILSH VGAGSDHHIP TNEILTSLSK DVLKSGKLKE
310 320 330 340 350
VHTKEEIGCS HPNCPLRAAI VIPLEMHGSI VGTLKMYFTN PNDLTFVERQ
360 370 380 390 400
LAEGLANIFS SQIELGEAET QSKLLKDAEI KSLQAQVSPH FFFNSINTIS
410 420 430 440 450
ALVRINSEKA RELLLELSYF FRANLQGSKQ HTITLDKELS QVRAYLSLEQ
460 470 480 490 500
ARYPGRFNIN INVEDKYRDV LVPPFLIQIL VENAIKHAFT NRKQGNDIDV
510 520 530 540 550
SVIKETATHV RIIVQDNGQG ISKDKMHLLG ETSVESESGT GSALENLNLR
560 570 580
LKGLFGKSAA LQFESTSSGT TFWCVLPYER QEEE
Length:584
Mass (Da):65,029
Last modified:July 11, 2006 - v2
Checksum:i6E8342DEF0057E43
GO

Sequence cautioni

The sequence ABD29405 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198T → P in AAB48182 (PubMed:8550490).Curated1
Sequence conflicti207S → P in AAB48182 (PubMed:8550490).Curated1
Sequence conflicti213E → D in AAB48182 (PubMed:8550490).Curated1
Sequence conflicti217A → P in AAB48182 (PubMed:8550490).Curated1
Sequence conflicti231L → F in AAB48182 (PubMed:8550490).Curated1
Sequence conflicti258A → S in AAB48182 (PubMed:8550490).Curated1
Sequence conflicti273A → G in AAB48182 (PubMed:8550490).Curated1
Sequence conflicti398T → P in AAB48182 (PubMed:8550490).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42945 Genomic DNA. Translation: AAB48182.1.
CP000253 Genomic DNA. Translation: ABD29405.1. Different initiation.
U57060 Genomic DNA. Translation: AAB81288.1.
RefSeqiWP_000925394.1. NC_007795.1.
YP_498825.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29405; ABD29405; SAOUHSC_00230.
GeneIDi3920305.
KEGGisao:SAOUHSC_00230.
PATRICi19578070. VBIStaAur99865_0211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42945 Genomic DNA. Translation: AAB48182.1.
CP000253 Genomic DNA. Translation: ABD29405.1. Different initiation.
U57060 Genomic DNA. Translation: AAB81288.1.
RefSeqiWP_000925394.1. NC_007795.1.
YP_498825.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ53705.
SMRiQ53705.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_00230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29405; ABD29405; SAOUHSC_00230.
GeneIDi3920305.
KEGGisao:SAOUHSC_00230.
PATRICi19578070. VBIStaAur99865_0211.

Phylogenomic databases

eggNOGiENOG4108HRK. Bacteria.
COG3275. LUCA.
HOGENOMiHOG000242218.
KOiK07704.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR010559. Sig_transdc_His_kin_internal.
IPR011620. Sig_transdc_His_kinase_LytS_TM.
[Graphical view]
PfamiPF07694. 5TM-5TMR_LYT. 1 hit.
PF02518. HATPase_c. 1 hit.
PF06580. His_kinase. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 1 hit.
SSF55874. SSF55874. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLYTS_STAA8
AccessioniPrimary (citable) accession number: Q53705
Secondary accession number(s): P72361, Q2G1B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: July 11, 2006
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.