Reviewed, UniProtKB/Swiss-Prot Q53685 (OLED_STRAT)

Names and origin

Protein names

Recommended name:
    Oleandomycin glycosyltransferase
    EC=2.4.1.-

Gene names

Name:	oleD
Synonyms:	UGT102A2

Organism

Streptomyces antibioticus

Taxonomic identifier

1890 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specifically inactivates oleandomycin via 2'-O-glycosylation using UDP-glucose.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	antibiotic metabolic process Inferred from electronic annotation. Source: InterPro carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 430

430

Oleandomycin glycosyltransferase

PRO_0000074165

Secondary structure

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430

Helix Strand Turn

Details...

Beta strand

9 – 13

5

Helix

18 – 21

4

Helix

22 – 24

3

Helix

25 – 33

9

Beta strand

37 – 42

6

Helix

44 – 46

3

Helix

47 – 51

5

Beta strand

56 – 59

4

Helix

71 – 74

4

Helix

78 – 100

23

Beta strand

106 – 111

6

Helix

115 – 124

10

Beta strand

128 – 134

7

Helix

141 – 144

4

Helix

146 – 155

10

Helix

157 – 172

16

Helix

179 – 184

6

Beta strand

187 – 191

5

Helix

195 – 197

3

Helix

201 – 203

3

Turn

206 – 208

3

Beta strand

209 – 211

3

Beta strand

232 – 238

7

Turn

240 – 242

3

Helix

247 – 257

11

Beta strand

263 – 268

6

Helix

275 – 278

4

Beta strand

285 – 290

6

Helix

293 – 297

5

Beta strand

301 – 305

5

Helix

309 – 317

9

Beta strand

322 – 324

3

Helix

329 – 331

3

Helix

332 – 340

9

Beta strand

343 – 346

4

Helix

354 – 366

13

Helix

368 – 384

17

Helix

386 – 395

10

Sequences

	Sequence		Length	Mass (Da)
	Q53685-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 320512217B42A0B7	FASTA	430	47,136
	10 20 30 40 50 60 MTTQTTPAHI AMFSIAAHGH VNPSLEVIRE LVARGHRVTY AIPPVFADKV AATGPRPVLY 70 80 90 100 110 120 HSTLPGPDAD PEAWGSTLLD NRRTFLNDAI QALPQLADAY ADDIPDLVLH DITSYPARVL 130 140 150 160 170 180 ARRWGVPAVS LSPNLVAWKG YEEEVAEPMW REPRQTERGR AYYARFEAWL KENGITEHPD 190 200 210 220 230 240 TFASHPPRSL VLIPKALQPH ADRVDEDVYT FVGACQGDRA EEGGWQRPAG AEKVVLVSLG 250 260 270 280 290 300 SAFTKQPAFY RECVRAFGNL PGWHLVLQIG RKVTPAELGE LPDNVEVHDW VPQLAILRQA 310 320 330 340 350 360 DLFVTHAGAG GSQEGLATAT PMIAVPQAVD QFGNADMLQG LGVARKLATE EATADLLRET 370 380 390 400 410 420 ALALVDDPEV ARRLRRIQAE MAQEGGTRRA ADLIEAELPA RHERQEPVGD RPNVGDRPAG 430 VRSDRQRSAL

References

[1]	"Characterization of a Streptomyces antibioticus gene cluster encoding a glycosyltransferase involved in oleandomycin inactivation." Hernandez C., Olano C., Mendez C., Salas J.A. Gene 134:139-140(1993) [PubMed: 8244027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11891 / DSM 40868 / NCIB 11506.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z22577 Genomic DNA. Translation: CAA80301.1.

PIR

S33184.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IYF	X-ray	1.70	A/B	1-430	[»]

ModBase

Search...

Protein family/group databases

CAZy

GT1. Glycosyltransferase Family 1.

Family and domain databases

InterPro

IPR004276. Glyco_trans_28.
IPR007235. Glyco_trans_28_C.
IPR002213. UDP_glucos_trans.
IPR006326. UDPGT_MGT.
[Graphical view]

PANTHER

PTHR11926. UDP_glucos_trans. 1 hit.

Pfam

PF04101. Glyco_tran_28_C. 1 hit.
PF03033. Glyco_transf_28. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01426. MGT. 1 hit.

PROSITE

PS00375. UDPGT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

OLED_STRAT

Accession

Primary (citable) accession number: Q53685

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)