Q53672 (Q53672_STRAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
MtmK protein EMBL CAA61990.1

Gene names

Name:

mtmK EMBL CAA61990.1

Organism

Streptomyces argillaceus EMBL CAA61990.1

Taxonomic identifier

41951 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the beta-ketoacyl-ACP synthases family. RuleBase RU003694

Ontologies

Keywords
Molecular function	Transferase RuleBase RU003694
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q53672 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B57E7F8B480373DB	FASTA	408	42,269
	10 20 30 40 50 60 MSADASQAVI TGIGVAAPNG LSVKAWWDAV LRGESGIRRL SRFDPGRYPA RLAGEIRDFV 70 80 90 100 110 120 DADHVPGRLL PQTDRVTRLS LAVAREAVED AGVDLERLPR YAAGVSTASS AGGFEFGQRE 130 140 150 160 170 180 LQALWSKGGQ YVSAYQSFAW FYAVNTGQIS IGYDLRGPSG VLVTEQAGGL DAVAQARRLL 190 200 210 220 230 240 RRGSELMVTG GVDSALCPWG WTAHLAGGRL STGDDPARAY LPFSADADGE VVGEGGALLV 250 260 270 280 290 300 LERAAAAPSR GARVYGVFAG YAATFDPPPG RGGTPQLARA AELALVDAGM TPDQVDVVFA 310 320 330 340 350 360 DAAGERAADR AEADALTRVF GARGVPVTAP KTMTGRLSAG GASLDLAAAL LALRDQVVPP 370 380 390 400 TVNVTEPADD CPVDLVTGRP RPLPLRAALV LARGRGGFNA AAVVRALS

References

[1]	"Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin." Lombo F., Blanco G., Fernandez E., Mendez C., Salas J.A. Gene 172:87-91(1996) [PubMed: 8654997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[2]	"An ABC transporter is essential for resistance to the antitumor agent mithramycin in the producer Streptomyces argillaceus." Fernandez E., Lombo F., Mendez C., Salas J.A. Mol. Gen. Genet. 251:692-698(1996) [PubMed: 8757400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[3]	"Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin." Lombo F., Siems K., Brana A.F., Mendez C., Bindseil K., Salas J.A. J. Bacteriol. 179:3354-3357(1997) [PubMed: 9150235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[4]	"Identification of two genes from Streptomyces argillaceus encoding glycosyltransferases involved in transfer of a disaccharide during biosynthesis of the antitumor drug mithramycin." Fernandez E., Weissbach U., Sanchez Reillo C., Brana A.F., Mendez C., Rohr J., Salas J.A. J. Bacteriol. 180:4929-4937(1998) [PubMed: 9733697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[5]	"Analysis of two chromosomal regions adjacent to genes for a type II polyketide synthase involved in the biosynthesis of the antitumor polyketide mithramycin in Streptomyces argillaceus." Prado L., Lombo F., Brana A.F., Mendez C., Rohr J., Salas J.A. Mol. Gen. Genet. 261:216-225(1999) [PubMed: 10102355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[6]	"Oxidative cleavage of premithramycin B is one of the last steps in the biosynthesis of the antitumor drug mithramycin." Prado L., Fernandez E., Weissbach U., Blanco G., Quiros L.M., Brana A.F., Mendez C., Rohr J., Salas J.A. Chem. Biol. 6:19-30(1999) [PubMed: 9889148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[7]	"Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus." Blanco G., Fernandez E., Fernandez M.J., Brana A.F., Weissbach U., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 262:991-1000(2000) [PubMed: 10660060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[8]	"The mtmVUC genes of the mithramycin gene cluster in Streptomyces argillaceus are involved in the biosynthesis of the sugar moieties." Gonzalez A., Remsing L.L., Lombo F., Fernandez M.J., Prado L., Brana A.F., Kunzel E., Rohr J., Mendez C., Salas J.A. Mol. Gen. Genet. 264:827-835(2001) [PubMed: 11254130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[9]	"Mithramycin SK, a novel antitumor drug with improved therapeutic index, mithramycin SA, and demycarosyl-mithramycin SK: three new products generated in the mithramycin producer Streptomyces argillaceus through combinatorial biosynthesis." Remsing L.L., Gonzalez A.M., Nur-e-Alam M., Fernandez-Lozano M.J., Brana A.F., Rix U., Oliveira M.A., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 125:5745-5753(2003) [PubMed: 12733914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[10]	"The structure of mithramycin reinvestigated." Wohlert S.E., Kunzel E., Machinek R., Mendez C., Salas J.A., Rohr J. J. Nat. Prod. 62:119-121(1999) [PubMed: 9917296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[11]	"Ketopremithramycins and ketomithramycins, four new aureolic acid-type compounds obtained upon inactivation of two genes involved in the biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring steps of the mithramycin biosynthetic pathway." Remsing L.L., Garcia-Bernardo J., Gonzalez A., Kunzel E., Rix U., Brana A.F., Bearden D.W., Mendez C., Salas J.A., Rohr J. J. Am. Chem. Soc. 124:1606-1614(2002) [PubMed: 11853433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[12]	"Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin by Streptomyces argillaceus." Lozano M.J., Remsing L.L., Quiros L.M., Brana A.F., Fernandez E., Sanchez C., Mendez C., Rohr J., Salas J.A. J. Biol. Chem. 275:3065-3074(2000) [PubMed: 10652287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[13]	"Insertional inactivation of mtrX and mtrY genes from the mithramycin gene cluster affects production and growth of the producer organism Streptomyces argillaceus." Garcia-Bernardo J., Brana A.F., Mendez C., Salas J.A. FEMS Microbiol. Lett. 186:61-65(2000) [PubMed: 10779713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[14]	"Rationally designed glycosylated premithramycins: hybrid aromatic polyketides using genes from three different biosynthetic pathways." Trefzer A., Blanco G., Remsing L., Kunzel E., Rix U., Lipata F., Brana A.F., Mendez C., Rohr J., Bechthold A., Salas J.A. J. Am. Chem. Soc. 124:6056-6062(2002) [PubMed: 12022840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[15]	"Purification and characterization of a monooxygenase involved in the biosynthetic pathway of the antitumor drug mithramycin." Rodriguez D., Quiros L.M., Brana A.F., Salas J.A. J. Bacteriol. 185:3962-3965(2003) [PubMed: 12813091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[16]	"Deciphering the biosynthetic origin of the aglycone of the aureolic acid group of anti-tumor agents." Blanco G., Fu H., Mendez C., Khosla C., Salas J.A. Chem. Biol. 3:193-196(1996) [PubMed: 8807845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[17]	"MtmMII-mediated C-methylation during biosynthesis of the antitumor drug mithramycin is essential for biological activity and DNA-drug interaction." Rodriguez D., Quiros L.M., Salas J.A. J. Biol. Chem. 279:8149-8158(2004) [PubMed: 14660589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[18]	"Folding of the polyketide chain is not dictated by minimal polyketide synthase in the biosynthesis of mithramycin and anthracycline." Kantola J., Blanco G., Hautala A., Kunnari T., Hakala J., Mendez C., Ylihonko K., Mantsala P., Salas J. Chem. Biol. 4:751-755(1997) [PubMed: 9375253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[19]	"Elucidation of the glycosylation sequence of mithramycin biosynthesis: isolation of 3A-deolivosylpremithramycin B and its conversion to premithramycin B by glycosyltransferase MtmGII." Nur-e-Alam M., Mendez C., Salas J.A., Rohr J. Chembiochem 6:632-636(2005) [PubMed: 15712316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[20]	"The Novel Hybrid Antitumor Compound Premithramycinone H Provides Indirect Evidence for a Tricyclic Intermediate of the Biosynthesis of the Aureolic Acid Antibiotic Mithramycin." Lombo F., Kunzel E., Prado L., Brana A.F., Bindseil K.U., Frevert J., Bearden D., Mendez C., Salas J.A., Rohr J. Angew. Chem. Int. Ed. 39:796-799(2000) [PubMed: 10760873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
[21]	Lombo F. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 12956 EMBL CAA61990.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89899 Genomic DNA. Translation: CAA61990.1.
PIR	S58174.
3D structure databases
HSSP	HSSP built from PDB template 1KAS based on UniProtKB P39435.
ProteinModelPortal	Q53672.
SMR	Q53672. Positions 8-406.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000794. Beta-ketoacyl_synthase. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00109. ketoacyl-synt. 1 hit. PF02801. Ketoacyl-synt_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

Q53672_STRAA

Accession

Primary (citable) accession number: Q53672

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	April 5, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)