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Q53654 (CNA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Collagen adhesin
Gene names
Name:cna
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus

Protein attributes

Sequence length1183 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates attachment of staphylococcal cells to collagen-containing substrata.

Subcellular location

Secreted › cell wall; Peptidoglycan-anchor Potential.

Ontologies

Keywords
   Cellular componentCell wall
Secreted
   DomainRepeat
Signal
   PTMIsopeptide bond
Peptidoglycan-anchor
   Technical term3D-structure
Gene Ontology (GO)
   Cellular_componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 11541125Collagen adhesin
PRO_0000005599
Propeptide1155 – 118329Removed by sortase Potential
PRO_0000005600

Regions

Repeat533 – 719187B1
Repeat720 – 906187B2
Repeat907 – 1093187B3
Region151 – 318168Collagen-binding
Region533 – 10935613 X 187 AA approximate tandem repeats
Motif1151 – 11555LPXTG sorting signal Potential
Compositional bias1093 – 115765Lys/Pro-rich

Sites

Site2091Autocatalyzes isopeptide 176-293 formation Potential
Site6011Autocatalyzes isopeptide 541-618 formation Potential
Site6921Autocatalyzes isopeptide 631-715 formation Potential
Site7881Autocatalyzes isopeptide 728-805 formation Potential
Site8791Autocatalyzes isopeptide 818-902 formation Potential
Site9751Autocatalyzes isopeptide 915-992 formation Potential
Site10661Autocatalyzes isopeptide 1005-1089 formation Potential

Amino acid modifications

Modified residue11541Pentaglycyl murein peptidoglycan amidated threonine Potential
Cross-link176 ↔ 293Isoaspartyl lysine isopeptide (Lys-Asn) Potential
Cross-link541 ↔ 618Isoaspartyl lysine isopeptide (Lys-Asn) Potential
Cross-link631 ↔ 715Isoaspartyl lysine isopeptide (Lys-Asn) Potential
Cross-link728 ↔ 805Isoaspartyl lysine isopeptide (Lys-Asn) Potential
Cross-link818 ↔ 902Isoaspartyl lysine isopeptide (Lys-Asn) Potential
Cross-link915 ↔ 992Isoaspartyl lysine isopeptide (Lys-Asn) Potential
Cross-link1005 ↔ 1089Isoaspartyl lysine isopeptide (Lys-Asn) Potential

Secondary structure

1 .............................................................................................................................. 1183
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q53654 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B6A1CC072E575D76

FASTA1,183133,067
        10         20         30         40         50         60 
MNKNVLKFMV FIMLLNIITP LFNKNEAFAA RDISSTNVTD LTVSPSKIED GGKTTVKMTF 

        70         80         90        100        110        120 
DDKNGKIQNG DMIKVAWPTS GTVKIEGYSK TVPLTVKGEQ VGQAVITPDG ATITFNDKVE 

       130        140        150        160        170        180 
KLSDVSGFAE FEVQGRNLTQ TNTSDDKVAT ITSGNKSTNV TVHKSEAGTS SVFYYKTGDM 

       190        200        210        220        230        240 
LPEDTTHVRW FLNINNEKSY VSKDITIKDQ IQGGQQLDLS TLNINVTGTH SNYYSGQSAI 

       250        260        270        280        290        300 
TDFEKAFPGS KITVDNTKNT IDVTIPQGYG SYNSFSINYK TKITNEQQKE FVNNSQAWYQ 

       310        320        330        340        350        360 
EHGKEEVNGK SFNHTVHNIN ANAGIEGTVK GELKVLKQDK DTKAPIANVK FKLSKKDGSV 

       370        380        390        400        410        420 
VKDNQKEIEI ITDANGIANI KALPSGDYIL KEIEAPRPYT FDKDKEYPFT MKDTDNQGYF 

       430        440        450        460        470        480 
TTIENAKAIE KTKDVSAQKV WEGTQKVKPT IYFKLYKQDD NQNTTPVDKA EIKKLEDGTT 

       490        500        510        520        530        540 
KVTWSNLPEN DKNGKAIKYL VKEVNAQGED TTPEGYTKKE NGLVVTNTEK PIETTSISGE 

       550        560        570        580        590        600 
KVWDDKDNQD GKRPEKVSVN LLANGEKVKT LDVTSETNWK YEFKDLPKYD EGKKIEYTVT 

       610        620        630        640        650        660 
EDHVKDYTTD INGTTITNKY TPGETSATVT KNWDDNNNQD GKRPTEIKVE LYQDGKATGK 

       670        680        690        700        710        720 
TAILNESNNW THTWTGLDEK AKGQQVKYTV EELTKVKGYT THVDNNDMGN LIVTNKYTPE 

       730        740        750        760        770        780 
TTSISGEKVW DDKDNQDGKR PEKVSVNLLA DGEKVKTLDV TSETNWKYEF KDLPKYDEGK 

       790        800        810        820        830        840 
KIEYTVTEDH VKDYTTDING TTITNKYTPG ETSATVTKNW DDNNNQDGKR PTEIKVELYQ 

       850        860        870        880        890        900 
DGKATGKTAI LNESNNWTHT WTGLDEKAKG QQVKYTVEEL TKVKGYTTHV DNNDMGNLIV 

       910        920        930        940        950        960 
TNKYTPETTS ISGEKVWDDK DNQDGKRPEK VSVNLLANGE KVKTLDVTSE TNWKYEFKDL 

       970        980        990       1000       1010       1020 
PKYDEGKKIE YTVTEDHVKD YTTDINGTTI TNKYTPGETS ATVTKNWDDN NNQDGKRPTE 

      1030       1040       1050       1060       1070       1080 
IKVELYQDGK ATGKTAILNE SNNWTHTWTG LDEKAKGQQV KYTVDELTKV NGYTTHVDNN 

      1090       1100       1110       1120       1130       1140 
DMGNLIVTNK YTPKKPNKPI YPEKPKDKTP PTKPDHSNKV KPTPPDKPSK VDKDDQPKDN 

      1150       1160       1170       1180 
KTKPENPLKE LPKTGMKIIT SWITWVFIGI LGLYLILRKR FNS

« Hide

References

[1]"Molecular characterization and expression of a gene encoding a Staphylococcus aureus collagen adhesin."
Patti J.M., Jonsson H., Guss B., Switalski L.M., Wiberg K., Lindberg M., Hoeoek M.
J. Biol. Chem. 267:4766-4772(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: FDA 574.
[2]Erratum
Patti J.M., Jonsson H., Guss B., Switalski L.M., Wiberg K., Lindberg M., Hoeoek M.
J. Biol. Chem. 269:11672-11672(1994)
[3]"Identification and biochemical characterization of the ligand binding domain of the collagen adhesin from Staphylococcus aureus."
Patti J.M., Boles J.O., Hoeoek M.
Biochemistry 32:11428-11435(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN COLLAGEN-BINDING.
Strain: FDA 574.
[4]"Stabilizing isopeptide bonds revealed in Gram-positive bacterial pilus structure."
Kang H.J., Coulibaly F., Clow F., Proft T., Baker E.N.
Science 318:1625-1628(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CROSS-LINKS.
[5]"Structure of the collagen-binding domain from a Staphylococcus aureus adhesin."
Symersky J., Patti J.M., Carson M., House-Pompeo K., Teale M., Moore D., Jin L., Schneider A., DeLucas L.J., Hoeoek M., Narayana S.V.L.
Nat. Struct. Biol. 4:833-838(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 169-318.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M81736 Genomic DNA. Translation: AAA20874.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AMXX-ray2.00A151-318[»]
1D2OX-ray2.00A/B533-719[»]
1D2PX-ray2.50A533-905[»]
2F68X-ray1.95X30-334[»]
2F6AX-ray3.29A/B/C/D30-330[»]
DisProtDP00098.
ProteinModelPortalQ53654.
SMRQ53654. Positions 31-329, 483-1094.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.1140. 7 hits.
2.60.40.740. 1 hit.
InterProIPR008966. Adhesion_dom.
IPR008454. Collagen-bd_Cna-like_B-typ_dom.
IPR008970. Collagen-bd_Cna_B-typ_dom.
IPR008456. Collagen-bd_dom.
IPR019931. LPXTG-motif_cell_wall_anchor.
[Graphical view]
PfamPF05738. Cna_B. 7 hits.
PF05737. Collagen_bind. 1 hit.
[Graphical view]
SUPFAMSSF49401. Adhes_bact. 2 hits.
SSF49478. Cna_B_unit. 7 hits.
TIGRFAMsTIGR01167. LPXTG_anchor. 1 hit.
PROSITEPS50847. GRAM_POS_ANCHORING. False negative.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ53654.

Entry information

Entry nameCNA_STAAU
AccessionPrimary (citable) accession number: Q53654
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents