Q53641 (Q53641_9CREN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Name:

treZ EMBL BAA11863.1

Organism

Sulfolobus acidocaldarius EMBL BAA11011.1

Taxonomic identifier

2285 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	253	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	284	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	376	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	Q53641 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4215B45E6C8ED4E7	FASTA	556	64,374
	10 20 30 40 50 60 MFSFGGNIEK NKGIFKLWAP YVNSVKLKLS KKLIPMEKND EGFFEVEIDD IEENLTYSYI 70 80 90 100 110 120 IEDKREIPDP ASRYQPLGVH DKSQLIRTDY QILDLGKVKI EDLIIYELHV GTFSQEGNFK 130 140 150 160 170 180 GVIEKLDYLK DLGITGIELM PVAQFPGNRD WGYDGVFLYA VQNTYGGPWE LAKLVNEAHK 190 200 210 220 230 240 RGIAVILDVV YNHIGPEGNY LLGLGPYFSD RYKTPWGLTF NFDDRGCDQV RKFILENVEY 250 260 270 280 290 300 WFKTFKIDGL RLDAVHAIFD NSPKHILQEI AEKAHQLGKF VIAESDLNDP KIVKDDCGYK 310 320 330 340 350 360 IDAQWVDDFH HAVHAFITKE KDYYYQDFGR IEDIEKTFKD VFVYDGKYSR YRGRTHGAPV 370 380 390 400 410 420 GDLPPRKFVV FIQNHDQVGN RGNGERLSIL TDKTTYLMAA TLYILSPYIP LIFMGEEYYE 430 440 450 460 470 480 TNPFFFFSDF SDPVLIKGVR EGRLKENNQM IDPQSEEAFL KSKLSWKIDE EVLDYYKQLI 490 500 510 520 530 540 NIRKRYNNCK RVKEVRREGN CITLIMEKIG IIASFDDIVI NSKITGNLLI GIGFPKKLKK 550 DELIKVNRGV GVYQLE

References

[1]	"Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldarius." Maruta K., Mitsuzumi H., Nakada T., Kubota M., Chaen H., Fukuda S., Sugimoto T., Kurimoto M. Biochim. Biophys. Acta 1291:177-181(1996) [PubMed: 8980629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC33909 EMBL BAA11863.1.
[2]	"Gene analysis of trehalose-producing enzymes from hyperthermophilic archaea in Sulfolobales." Kobayashi K., Kato M., Miura Y., Kettoku M., Komeda T., Iwamatsu A. Biosci. Biotechnol. Biochem. 60:1720-1723(1996) [PubMed: 8987674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Cloning and sequencing of a cluster of trehalose biosynthesis genes from thermophilic archaebacterium Sulfolobus acidocaldarius." Maruta K., Mitsuzumi H., Nakada T., Kubota M., Chaen H., Fukuda S., Sugimoto T., Kurimoto M. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC33909 EMBL BAA11863.1.
[4]	"The gene analysis of the new amylases from the hyper thermophilic archae Sulfolobus." Kato M., Kettoku M., Miura Y., Komeda T., Konishi Y., Shindo K., Kobayashi K., Iwamatsu A. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC33909 EMBL BAA11011.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D64131 Genomic DNA. Translation: BAA11011.1. D83245 Genomic DNA. Translation: BAA11863.1.
PIR	JC5132.
3D structure databases
HSSP	HSSP built from PDB template 1EH9 based on UniProtKB Q55088.
ProteinModelPortal	Q53641.
SMR	Q53641. Positions 3-554.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
NMPDR	fig|330779.3.peg.1987.
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK285578.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1440-MONOMER.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR015156. Maltooligo_trehalose_arc_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF09071. Alpha-amyl_C. 1 hit. PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q53641_9CREN

Accession

Primary (citable) accession number: Q53641
Secondary accession number(s): O08064, O08279, Q4J8W4

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)