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Q53609 (MTS1_STRAL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase SalI

Short name=M.SalI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase SalI
Gene names
Name:salIM
OrganismStreptomyces albus G
Taxonomic identifier1962 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the SalI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587Modification methylase SalI
PRO_0000087979

Sequences

Sequence LengthMass (Da)Tools
Q53609 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B482F374397FF999

FASTA58764,975
        10         20         30         40         50         60 
MHSEAREAEA RRLLLQETLD AERTFLERNQ WGQFATPPSL AGEIMRYTID LHEETRINFL 

        70         80         90        100        110        120 
EPSCGSGSFF SALLRNLGDK KIEHAVGVEL DPRFSKAASD LWTGQGLRVI EGDFTSPSLV 

       130        140        150        160        170        180 
SGPVASLLVA NPPYVRHHHL GIDQKRDLVA RCADQLGIKP SGLSGLYLYF VLLSHRLLRA 

       190        200        210        220        230        240 
DAVSTWLIPS EFMDVNYGTA LKEYLATRVQ LVRIHQYDAA EVQFDDALVT SSVVVFRNSP 

       250        260        270        280        290        300 
PRPGHTAEFS FGGTLSEPKV THQIPSAALT PEAKWSRYVT GVMPADINLK QTGPKLSDFF 

       310        320        330        340        350        360 
KIRRGLATGS NAFFIIPRSE AERLGIKRNF LRPILPSPRK LKGDAITADA SGWPDIPEQL 

       370        380        390        400        410        420 
ALLDCPLPIE DLLLENPALA AYLSTADEKI RGGYLVSKRS PWYKQEQREP APILLTYMGR 

       430        440        450        460        470        480 
GKDDQHPLRF IRNDSDAVAT NMYLMLYPTA LLQRYLAGDP ERIKQVHKAL LAITAADLRG 

       490        500        510        520        530        540 
GGRVYGGGLH KMEPKELAAL PADGIATLDP VLREDISMVS VPPRKRTGRP QMPGPSASEV 

       550        560        570        580 
RAWARANGVC VPDRGRLRPE VWDAWRQAHA GEASPLNIDA GDQVALW 

« Hide

References

[1]"Organization and sequence of the SalI restriction-modification system."
Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G.
Gene 151:167-172(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U01232 Genomic DNA. Translation: AAA81887.1.

3D structure databases

ProteinModelPortalQ53609.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3491. M.SalI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR024412. Lsr2.
IPR002296. N12N6_MeTrfase.
[Graphical view]
PfamPF11774. Lsr2. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
ProDomPD026347. PD026347. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTS1_STRAL
AccessionPrimary (citable) accession number: Q53609
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries