Q53609 (MTS1_STRAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase SalI
Short name=M.SalI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase SalI

Gene names

Name:

salIM

Organism

Streptomyces albus G

Taxonomic identifier

1962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces albus

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the SalI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 587	587	Modification methylase SalI		PRO_0000087979

Sequences

	Sequence		Length	Mass (Da)
	Q53609 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B482F374397FF999	FASTA	587	64,975
	10 20 30 40 50 60 MHSEAREAEA RRLLLQETLD AERTFLERNQ WGQFATPPSL AGEIMRYTID LHEETRINFL 70 80 90 100 110 120 EPSCGSGSFF SALLRNLGDK KIEHAVGVEL DPRFSKAASD LWTGQGLRVI EGDFTSPSLV 130 140 150 160 170 180 SGPVASLLVA NPPYVRHHHL GIDQKRDLVA RCADQLGIKP SGLSGLYLYF VLLSHRLLRA 190 200 210 220 230 240 DAVSTWLIPS EFMDVNYGTA LKEYLATRVQ LVRIHQYDAA EVQFDDALVT SSVVVFRNSP 250 260 270 280 290 300 PRPGHTAEFS FGGTLSEPKV THQIPSAALT PEAKWSRYVT GVMPADINLK QTGPKLSDFF 310 320 330 340 350 360 KIRRGLATGS NAFFIIPRSE AERLGIKRNF LRPILPSPRK LKGDAITADA SGWPDIPEQL 370 380 390 400 410 420 ALLDCPLPIE DLLLENPALA AYLSTADEKI RGGYLVSKRS PWYKQEQREP APILLTYMGR 430 440 450 460 470 480 GKDDQHPLRF IRNDSDAVAT NMYLMLYPTA LLQRYLAGDP ERIKQVHKAL LAITAADLRG 490 500 510 520 530 540 GGRVYGGGLH KMEPKELAAL PADGIATLDP VLREDISMVS VPPRKRTGRP QMPGPSASEV 550 560 570 580 RAWARANGVC VPDRGRLRPE VWDAWRQAHA GEASPLNIDA GDQVALW

References

[1]	"Organization and sequence of the SalI restriction-modification system." Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G. Gene 151:167-172(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01232 Genomic DNA. Translation: AAA81887.1.
3D structure databases
ProteinModelPortal	Q53609.
ModBase	Search...
Protein family/group databases
REBASE	3491. M.SalI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR024412. Lsr2. IPR002296. N12N6_MeTrfase. [Graphical view]
Pfam	PF11774. Lsr2. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
ProDom	PD026347. PD026347. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTS1_STRAL

Accession

Primary (citable) accession number: Q53609

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program