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Protein

Modification methylase SalI

Gene

salIM

Organism
Streptomyces albus G
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the SalI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3491. M.SalI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SalI (EC:2.1.1.72)
Short name:
M.SalI
Alternative name(s):
Adenine-specific methyltransferase SalI
Gene namesi
Name:salIM
OrganismiStreptomyces albus G
Taxonomic identifieri1962 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587Modification methylase SalIPRO_0000087979Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ53609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR024412. Lsr2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF11774. Lsr2. 1 hit.
[Graphical view]
ProDomiPD026347. PD026347. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q53609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSEAREAEA RRLLLQETLD AERTFLERNQ WGQFATPPSL AGEIMRYTID
60 70 80 90 100
LHEETRINFL EPSCGSGSFF SALLRNLGDK KIEHAVGVEL DPRFSKAASD
110 120 130 140 150
LWTGQGLRVI EGDFTSPSLV SGPVASLLVA NPPYVRHHHL GIDQKRDLVA
160 170 180 190 200
RCADQLGIKP SGLSGLYLYF VLLSHRLLRA DAVSTWLIPS EFMDVNYGTA
210 220 230 240 250
LKEYLATRVQ LVRIHQYDAA EVQFDDALVT SSVVVFRNSP PRPGHTAEFS
260 270 280 290 300
FGGTLSEPKV THQIPSAALT PEAKWSRYVT GVMPADINLK QTGPKLSDFF
310 320 330 340 350
KIRRGLATGS NAFFIIPRSE AERLGIKRNF LRPILPSPRK LKGDAITADA
360 370 380 390 400
SGWPDIPEQL ALLDCPLPIE DLLLENPALA AYLSTADEKI RGGYLVSKRS
410 420 430 440 450
PWYKQEQREP APILLTYMGR GKDDQHPLRF IRNDSDAVAT NMYLMLYPTA
460 470 480 490 500
LLQRYLAGDP ERIKQVHKAL LAITAADLRG GGRVYGGGLH KMEPKELAAL
510 520 530 540 550
PADGIATLDP VLREDISMVS VPPRKRTGRP QMPGPSASEV RAWARANGVC
560 570 580
VPDRGRLRPE VWDAWRQAHA GEASPLNIDA GDQVALW
Length:587
Mass (Da):64,975
Last modified:November 1, 1996 - v1
Checksum:iB482F374397FF999
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01232 Genomic DNA. Translation: AAA81887.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01232 Genomic DNA. Translation: AAA81887.1.

3D structure databases

ProteinModelPortaliQ53609.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3491. M.SalI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR024412. Lsr2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF11774. Lsr2. 1 hit.
[Graphical view]
ProDomiPD026347. PD026347. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Organization and sequence of the SalI restriction-modification system."
    Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G.
    Gene 151:167-172(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTS1_STRAL
AccessioniPrimary (citable) accession number: Q53609
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.