Q53608 (T2S1_STRAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme SalI
Short name=R.SalI
EC=3.1.21.4

Alternative name(s):
Endonuclease SalI
Type II restriction enzyme SalI

Gene names

Name:

salIR

Organism

Streptomyces albus G

Taxonomic identifier

1962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces albus

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GTCGAC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Type-2 restriction enzyme SalI		PRO_0000077365

Sequences

	Sequence		Length	Mass (Da)
	Q53608 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DA7C3CA88E924F07	FASTA	315	35,337
	10 20 30 40 50 60 MINADKPHRW NDDVQASVRL YNQWFLDAAP KAYRDTRQLT IDEVEQAFQR TANMTSITPE 70 80 90 100 110 120 VLKAHPKTLA TLRMSTAPPI ARDRLVGLSH GSKSLLDTME KGKLPPRMKG DVLDTHLAKM 130 140 150 160 170 180 CAVLTDLLDL DLFHWYPTGE PAEPRQRELA ATVVADRLCG AIADPIVRNA QERRQLALIE 190 200 210 220 230 240 EWLLARGYTK KTHSASLPLN TMQPGTFSFR QNVVVGSDLP VNIPVDAVIQ PHTPHSHKLP 250 260 270 280 290 300 ILIEAKSAGD FTNTNKRRKE EATKIHQLQL KYGNEISLTL FLCGYFNTGY LGYSAAEGLD 310 WVWEHRIDDL EAAGA

References

[1]	"Organization and sequence of the SalI restriction-modification system." Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G. Gene 151:167-172(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01232 Genomic DNA. Translation: AAA81886.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1588. SalI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019072. Restrct_endonuc_II_XamI. [Graphical view]
Pfam	PF09572. RE_XamI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S1_STRAL

Accession

Primary (citable) accession number: Q53608

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program