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Q53608 (T2S1_STRAL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme SalI

Short name=R.SalI
EC=3.1.21.4
Alternative name(s):
Endonuclease SalI
Type II restriction enzyme SalI
Gene names
Name:salIR
OrganismStreptomyces albus G
Taxonomic identifier1962 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GTCGAC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Type-2 restriction enzyme SalI
PRO_0000077365

Sequences

Sequence LengthMass (Da)Tools
Q53608 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DA7C3CA88E924F07

FASTA31535,337
        10         20         30         40         50         60 
MINADKPHRW NDDVQASVRL YNQWFLDAAP KAYRDTRQLT IDEVEQAFQR TANMTSITPE 

        70         80         90        100        110        120 
VLKAHPKTLA TLRMSTAPPI ARDRLVGLSH GSKSLLDTME KGKLPPRMKG DVLDTHLAKM 

       130        140        150        160        170        180 
CAVLTDLLDL DLFHWYPTGE PAEPRQRELA ATVVADRLCG AIADPIVRNA QERRQLALIE 

       190        200        210        220        230        240 
EWLLARGYTK KTHSASLPLN TMQPGTFSFR QNVVVGSDLP VNIPVDAVIQ PHTPHSHKLP 

       250        260        270        280        290        300 
ILIEAKSAGD FTNTNKRRKE EATKIHQLQL KYGNEISLTL FLCGYFNTGY LGYSAAEGLD 

       310 
WVWEHRIDDL EAAGA 

« Hide

References

[1]"Organization and sequence of the SalI restriction-modification system."
Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G.
Gene 151:167-172(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U01232 Genomic DNA. Translation: AAA81886.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1588. SalI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019072. Restrct_endonuc_II_XamI.
[Graphical view]
PfamPF09572. RE_XamI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2S1_STRAL
AccessionPrimary (citable) accession number: Q53608
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries