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Protein

Type-2 restriction enzyme SalI

Gene

salIR

Organism
Streptomyces albus G
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence GTCGAC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1588. SalI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SalI (EC:3.1.21.4)
Short name:
R.SalI
Alternative name(s):
Endonuclease SalI
Type II restriction enzyme SalI
Gene namesi
Name:salIR
OrganismiStreptomyces albus G
Taxonomic identifieri1962 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Type-2 restriction enzyme SalIPRO_0000077365Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019072. Restrct_endonuc_II_XamI.
[Graphical view]
PfamiPF09572. RE_XamI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q53608-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINADKPHRW NDDVQASVRL YNQWFLDAAP KAYRDTRQLT IDEVEQAFQR
60 70 80 90 100
TANMTSITPE VLKAHPKTLA TLRMSTAPPI ARDRLVGLSH GSKSLLDTME
110 120 130 140 150
KGKLPPRMKG DVLDTHLAKM CAVLTDLLDL DLFHWYPTGE PAEPRQRELA
160 170 180 190 200
ATVVADRLCG AIADPIVRNA QERRQLALIE EWLLARGYTK KTHSASLPLN
210 220 230 240 250
TMQPGTFSFR QNVVVGSDLP VNIPVDAVIQ PHTPHSHKLP ILIEAKSAGD
260 270 280 290 300
FTNTNKRRKE EATKIHQLQL KYGNEISLTL FLCGYFNTGY LGYSAAEGLD
310
WVWEHRIDDL EAAGA
Length:315
Mass (Da):35,337
Last modified:November 1, 1996 - v1
Checksum:iDA7C3CA88E924F07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01232 Genomic DNA. Translation: AAA81886.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01232 Genomic DNA. Translation: AAA81886.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1588. SalI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019072. Restrct_endonuc_II_XamI.
[Graphical view]
PfamiPF09572. RE_XamI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Organization and sequence of the SalI restriction-modification system."
    Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G.
    Gene 151:167-172(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2S1_STRAL
AccessioniPrimary (citable) accession number: Q53608
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.