Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Streptomyces antibioticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi514MagnesiumUniRule annotation1
Metal bindingi520MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BRENDAi2.7.6.5. 5974.
2.7.7.8. 5974.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
OrganismiStreptomyces antibioticus
Taxonomic identifieri1890 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003298871 – 740Polyribonucleotide nucleotidyltransferaseAdd BLAST740

Interactioni

Subunit structurei

Homotrimer.1 Publication

Structurei

Secondary structure

1740
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 13Combined sources8
Helixi15 – 17Combined sources3
Beta strandi19 – 29Combined sources11
Beta strandi33 – 41Combined sources9
Turni42 – 44Combined sources3
Beta strandi45 – 57Combined sources13
Beta strandi66 – 72Combined sources7
Helixi74 – 77Combined sources4
Helixi93 – 106Combined sources14
Helixi107 – 109Combined sources3
Beta strandi116 – 126Combined sources11
Helixi134 – 147Combined sources14
Beta strandi150 – 152Combined sources3
Beta strandi157 – 164Combined sources8
Beta strandi167 – 171Combined sources5
Helixi174 – 177Combined sources4
Beta strandi180 – 190Combined sources11
Beta strandi196 – 205Combined sources10
Helixi209 – 214Combined sources6
Helixi222 – 250Combined sources29
Helixi265 – 282Combined sources18
Helixi288 – 303Combined sources16
Turni307 – 310Combined sources4
Helixi314 – 335Combined sources22
Beta strandi351 – 355Combined sources5
Beta strandi358 – 369Combined sources12
Beta strandi372 – 382Combined sources11
Helixi383 – 385Combined sources3
Beta strandi386 – 388Combined sources3
Beta strandi391 – 394Combined sources4
Beta strandi397 – 405Combined sources9
Helixi408 – 411Combined sources4
Helixi422 – 435Combined sources14
Helixi436 – 438Combined sources3
Turni442 – 444Combined sources3
Beta strandi447 – 457Combined sources11
Helixi462 – 477Combined sources16
Beta strandi486 – 499Combined sources14
Beta strandi501 – 508Combined sources8
Helixi511 – 516Combined sources6
Beta strandi518 – 525Combined sources8
Beta strandi527 – 537Combined sources11
Helixi544 – 568Combined sources25
Helixi607 – 611Combined sources5
Beta strandi626 – 628Combined sources3
Helixi629 – 632Combined sources4
Beta strandi657 – 660Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3HX-ray2.60A1-740[»]
1E3PX-ray2.50A1-665[»]
ProteinModelPortaliQ53597.
SMRiQ53597.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53597.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini580 – 639KHUniRule annotationAdd BLAST60
Domaini651 – 723S1 motifUniRule annotationAdd BLAST73

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q53597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENETHYAEA VIDNGAFGTR TIRFETGRLA KQAAGSAVAY LDDDTMVLSA
60 70 80 90 100
TTASKNPKDQ LDFFPLTVDV EERMYAAGKI PGSFFRREGR PSEDAILTCR
110 120 130 140 150
LIDRPLRPSF KKGLRNEIQV VATIMALNPD HLYDVVAINA ASASTQLAGL
160 170 180 190 200
PFSGPYGGVR VALIRGQWVA FPTHTELEDA VFDMVVAGRV LEDGDVAIMM
210 220 230 240 250
VEAEATEKTV QLVKDGAEAP TEEVVAAGLD AAKPFIKVLC KAQADLAAKA
260 270 280 290 300
AKPTGEFPVP SSTTRTTSEA LSAAVRPELS AALTIAGKQD REAELDRVKA
310 320 330 340 350
LAAEKLLPEF EGREKEISAA YRPWPSSSSA ERVIKEKKRI DGRGVTDIRT
360 370 380 390 400
LAAEVEAIPR VHGSALFERG ETQILGVTTL NMLRMEQQLD TLSPVTRKPY
410 420 430 440 450
MHNYNFPPIS VGETGRVGSP KRREIGHGAL AERAIVPVLP TREEFPYAIR
460 470 480 490 500
QVSEALGSNG STSMGSVCAS TMSLLNAGVP LKAPVAGIAM GLISQEINGE
510 520 530 540 550
THYVALTDIL GAEDAFGDMD FKVAGTKEFV TALQLDTKLD GIPASVLAAA
560 570 580 590 600
LKQARDARLH ILDVMMEAID TPDEMSPNAP RIITVKIPVD KIGEVIGPKR
610 620 630 640 650
QMINQIQEDT GAEITIEDDG TIYIGAADGP AAEAARATIN GIANPTSPEV
660 670 680 690 700
GERILGSVVK TTTFGAFVSL LPGKDGLLHI SQIRKLAGGK RVENVEDVLG
710 720 730 740
VGQKVQVEIA EIDSRGKLSL IPVIEGEEAA SDEKKDDAEQ
Length:740
Mass (Da):79,160
Last modified:November 1, 1996 - v1
Checksum:iF07224C64018BA54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19858 Genomic DNA. Translation: AAB17498.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19858 Genomic DNA. Translation: AAB17498.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3HX-ray2.60A1-740[»]
1E3PX-ray2.50A1-665[»]
ProteinModelPortaliQ53597.
SMRiQ53597.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.7.6.5. 5974.
2.7.7.8. 5974.

Miscellaneous databases

EvolutionaryTraceiQ53597.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_STRAT
AccessioniPrimary (citable) accession number: Q53597
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.