SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q53591

- HYSA_STRA3

UniProt

Q53591 - HYSA_STRA3

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hyaluronate lyase

Gene
hylB, gbs1270
Organism
Streptococcus agalactiae serotype III (strain NEM316)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Cleaves hyaluronate chains at a beta-D-GalNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei429 – 4291
Active sitei479 – 4791
Active sitei488 – 4881

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. hyaluronate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Enzyme and pathway databases

BRENDAi4.2.2.1. 5917.

Protein family/group databases

CAZyiPL8. Polysaccharide Lyase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronate lyase (EC:4.2.2.1)
Alternative name(s):
Hyaluronidase
Short name:
HYase
Gene namesi
Name:hylB
Ordered Locus Names:gbs1270
OrganismiStreptococcus agalactiae serotype III (strain NEM316)
Taxonomic identifieri211110 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000823: Chromosome

Organism-specific databases

GenoListigbs1270.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040 Reviewed predictionAdd
BLAST
Chaini41 – 984944Hyaluronate lyasePRO_0000024932Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi211110.gbs1270.

Structurei

Secondary structure

1
984
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi185 – 1884
Beta strandi196 – 1994
Beta strandi200 – 2067
Helixi207 – 2093
Beta strandi210 – 2134
Beta strandi216 – 2194
Beta strandi223 – 2308
Beta strandi236 – 24510
Helixi251 – 26313
Helixi266 – 2683
Helixi274 – 29320
Helixi305 – 3073
Helixi313 – 33018
Turni336 – 3394
Helixi341 – 35717
Beta strandi367 – 3693
Helixi371 – 3755
Helixi377 – 38812
Helixi390 – 3923
Helixi395 – 40814
Beta strandi414 – 4163
Turni417 – 4204
Helixi427 – 44317
Helixi447 – 45711
Helixi458 – 4603
Beta strandi464 – 4718
Beta strandi476 – 4783
Turni479 – 4813
Turni485 – 4873
Helixi488 – 50316
Helixi512 – 52413
Helixi527 – 5293
Helixi537 – 5393
Helixi541 – 5455
Helixi551 – 56616
Helixi572 – 58716
Beta strandi589 – 5913
Helixi593 – 5964
Helixi600 – 61112
Beta strandi623 – 6275
Helixi628 – 6303
Beta strandi632 – 6376
Turni638 – 6414
Beta strandi642 – 6476
Beta strandi653 – 6553
Beta strandi660 – 6623
Turni667 – 6704
Beta strandi671 – 6777
Turni681 – 6844
Beta strandi685 – 6873
Helixi688 – 6914
Beta strandi694 – 6963
Beta strandi701 – 7033
Helixi712 – 7165
Turni717 – 7204
Helixi722 – 7265
Beta strandi736 – 75318
Beta strandi757 – 76812
Beta strandi771 – 78111
Beta strandi782 – 7843
Beta strandi788 – 7969
Beta strandi799 – 8013
Beta strandi804 – 8074
Beta strandi816 – 83116
Beta strandi839 – 85820
Helixi860 – 8634
Beta strandi873 – 88311
Beta strandi886 – 8883
Beta strandi891 – 8988
Helixi901 – 9099
Beta strandi912 – 9187
Beta strandi920 – 9278
Turni928 – 9314
Beta strandi932 – 9376
Beta strandi943 – 9453
Turni946 – 9483
Beta strandi949 – 9513
Beta strandi955 – 9628
Beta strandi965 – 9728
Turni973 – 9764
Beta strandi977 – 9815

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F1SX-ray2.10A171-984[»]
1I8QX-ray2.20A171-984[»]
1LXMX-ray2.20A171-984[»]
ProteinModelPortaliQ53591.
SMRiQ53591. Positions 171-984.

Miscellaneous databases

EvolutionaryTraceiQ53591.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG305085.
HOGENOMiHOG000008667.
KOiK01727.
OrthoDBiEOG6GBM67.

Family and domain databases

Gene3Di1.50.10.100. 1 hit.
2.60.220.10. 1 hit.
2.60.40.1380. 1 hit.
2.70.98.10. 1 hit.
InterProiIPR008929. Chondroitin_lyas.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR023295. Hyaluronate_lyase_beta_dom.
IPR014756. Ig_E-set.
IPR011071. Lyase_8-like_C.
IPR012970. Lyase_8_alpha_N.
IPR004103. Lyase_8_C.
IPR003159. Lyase_8_central_dom.
IPR012329. Lyase_8_N.
[Graphical view]
PfamiPF02278. Lyase_8. 1 hit.
PF02884. Lyase_8_C. 1 hit.
PF08124. Lyase_8_N. 1 hit.
[Graphical view]
SUPFAMiSSF48230. SSF48230. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF49863. SSF49863. 1 hit.
SSF74650. SSF74650. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53591-1 [UniParc]FASTAAdd to Basket

« Hide

MKQVVDNQTQ NKELVKNGDF NQTNPVSGSW SHTSAREWSA WIDKENTADK    50
SPIIQRTEQG QVSLSSDKGF RGAVTQKVNI DPTKKYEVKF DIETSNKAGQ 100
AFLRIMEKKD NNTRLWLSEM TSGTTNKHTL TKIYNPKLNV SEVTLELYYE 150
KGTGSATFDN ISMKAKGPKD SEHPQPVTTQ IEESVNTALN KNYVFNKADY 200
QYTLTNPSLG KIVGGILYPN ATGSTTVKIS DKSGKIIKEV PLSVTASTED 250
KFTKLLDKWN DVTIGNHVYD TNDSNMQKIN QKLDETNAKN IKTIKLDSNH 300
TFLWKDLDNL NNSAQLTATY RRLEDLAKQI TNPHSTIYKN EKAIRTVKES 350
LAWLHQNFYN VNKDIEGSAN WWDFEIGVPR SITATLALMN NYFTDAEIKT 400
YTDPIEHFVP DAGYFRKTLD NPFKALGGNL VDMGRVKIIE GLLRKDNTII 450
EKTSHSLKNL FTTATKAEGF YADGSYIDHT NVAYTGAYGN VLIDGLTQLL 500
PIIQETDYKI SNQELDMVYK WINQSFLPLI VKGELMDMSR GRSISREAAS 550
SHAAAVEVLR GFLRLANMSN EERNLDLKST IKTIITSNKF YNVFNNLKSY 600
SDIANMNKML NDSTVATKPL KSNLSTFNSM DRLAYYNAEK DFGFALSLHS 650
KRTLNYEGMN DENTRDWYTG DGMFYLYNSD QSHYSNHFWP TVNPYKMAGT 700
TEKDAKREDT TKEFMSKHSK DAKEKTGQVT GTSDFVGSVK LNDHFALAAM 750
DFTNWDRTLT AQKGWVILND KIVFLGSNIK NTNGIGNVST TIDQRKDDSK 800
TPYTTYVNGK TIDLKQASSQ QFTDTKSVFL ESKEPGRNIG YIFFKNSTID 850
IERKEQTGTW NSINRTSKNT SIVSNPFITI SQKHDNKGDS YGYMMVPNID 900
RTSFDKLANS KEVELLENSS KQQVIYDKNS QTWAVIKHDN QESLINNQFK 950
MNKAGLYLVQ KVGNDYQNVY YQPQTMTKTD QLAI 984
Length:984
Mass (Da):111,581
Last modified:January 17, 2003 - v2
Checksum:iAC7215ED6D5C2592
GO

Sequence cautioni

The sequence CAD46929.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451E → G in AAA56749. 1 Publication
Sequence conflicti155 – 1562SA → FV in AAA56749. 1 Publication
Sequence conflicti183 – 1831E → K in AAA56749. 1 Publication
Sequence conflicti246 – 2516ASTEDK → GSPEDN in AAA56749. 1 Publication
Sequence conflicti267 – 2671H → Y in AAA56749. 1 Publication
Sequence conflicti279 – 2802IN → LT in AAA56749. 1 Publication
Sequence conflicti288 – 2881A → G in AAA56749. 1 Publication
Sequence conflicti292 – 2932KT → EA in AAA56749. 1 Publication
Sequence conflicti300 – 3001H → R in AAA56749. 1 Publication
Sequence conflicti384 – 3841A → G in AAA56749. 1 Publication
Sequence conflicti387 – 3871A → S in AAA56749. 1 Publication
Sequence conflicti413 – 4131G → E in AAA56749. 1 Publication
Sequence conflicti420 – 4201D → V in AAA56749. 1 Publication
Sequence conflicti583 – 5831T → A in AAA56749. 1 Publication
Sequence conflicti609 – 6091M → L in AAA56749. 1 Publication
Sequence conflicti639 – 6391E → K in AAA56749. 1 Publication
Sequence conflicti666 – 6661D → G in AAA56749. 1 Publication
Sequence conflicti676 – 6761L → I in AAA56749. 1 Publication
Sequence conflicti688 – 6892FW → LG in AAA56749. 1 Publication
Sequence conflicti882 – 8821Q → L in AAA56749. 1 Publication
Sequence conflicti894 – 8941M → L in AAA56749. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15050 Genomic DNA. Translation: AAA56749.1.
AL766849 Genomic DNA. Translation: CAD46929.1. Different initiation.
PIRiA55137.
RefSeqiNP_735714.1. NC_004368.1.

Genome annotation databases

EnsemblBacteriaiCAD46929; CAD46929; CAD46929.
GeneIDi1030294.
KEGGisan:gbs1270.
PATRICi19638423. VBIStrAga3577_1300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15050 Genomic DNA. Translation: AAA56749.1 .
AL766849 Genomic DNA. Translation: CAD46929.1 . Different initiation.
PIRi A55137.
RefSeqi NP_735714.1. NC_004368.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F1S X-ray 2.10 A 171-984 [» ]
1I8Q X-ray 2.20 A 171-984 [» ]
1LXM X-ray 2.20 A 171-984 [» ]
ProteinModelPortali Q53591.
SMRi Q53591. Positions 171-984.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 211110.gbs1270.

Protein family/group databases

CAZyi PL8. Polysaccharide Lyase Family 8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD46929 ; CAD46929 ; CAD46929 .
GeneIDi 1030294.
KEGGi san:gbs1270.
PATRICi 19638423. VBIStrAga3577_1300.

Organism-specific databases

GenoListi gbs1270.

Phylogenomic databases

eggNOGi NOG305085.
HOGENOMi HOG000008667.
KOi K01727.
OrthoDBi EOG6GBM67.

Enzyme and pathway databases

BRENDAi 4.2.2.1. 5917.

Miscellaneous databases

EvolutionaryTracei Q53591.

Family and domain databases

Gene3Di 1.50.10.100. 1 hit.
2.60.220.10. 1 hit.
2.60.40.1380. 1 hit.
2.70.98.10. 1 hit.
InterProi IPR008929. Chondroitin_lyas.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR023295. Hyaluronate_lyase_beta_dom.
IPR014756. Ig_E-set.
IPR011071. Lyase_8-like_C.
IPR012970. Lyase_8_alpha_N.
IPR004103. Lyase_8_C.
IPR003159. Lyase_8_central_dom.
IPR012329. Lyase_8_N.
[Graphical view ]
Pfami PF02278. Lyase_8. 1 hit.
PF02884. Lyase_8_C. 1 hit.
PF08124. Lyase_8_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48230. SSF48230. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF49863. SSF49863. 1 hit.
SSF74650. SSF74650. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the gene for group B streptococcal hyaluronate lyase."
    Lin B., Hollingshead S.K., Coligan J.E., Egan M.L., Baker J.R., Pritchard D.G.
    J. Biol. Chem. 269:30113-30116(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 3502 / Serotype III.
  2. "Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease."
    Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.
    Mol. Microbiol. 45:1499-1513(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NEM316.
  3. "Hyaluronan binding and degradation by Streptococcus agalactiae hyaluronate lyase."
    Li S., Jedrzejas M.J.
    J. Biol. Chem. 276:41407-41416(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 171-984.

Entry informationi

Entry nameiHYSA_STRA3
AccessioniPrimary (citable) accession number: Q53591
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 17, 2003
Last modified: May 14, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi