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Protein

4-hydroxyphenylpyruvate dioxygenase

Gene

hpd

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.

Cofactori

Fe cationNote: Binds 1 Fe cation per subunit.

Pathwayi: L-phenylalanine degradation

This protein is involved in step 3 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 4-hydroxyphenylpyruvate dioxygenase (hpd)
  4. Homogentisate 1,2-dioxygenase (hmgA)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi187 – 1871Iron
Metal bindingi270 – 2701Iron
Metal bindingi349 – 3491Iron

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-5215-MONOMER.
BRENDAi1.13.11.27. 5980.
SABIO-RKQ53586.
UniPathwayiUPA00139; UER00362.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyphenylpyruvate dioxygenase (EC:1.13.11.27)
Short name:
4HPPD
Short name:
HPD
Short name:
HPPDase
Gene namesi
Name:hpd
Ordered Locus Names:SAV_5149
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3813814-hydroxyphenylpyruvate dioxygenasePRO_0000088409Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi227882.SAV_5149.

Structurei

Secondary structure

1
381
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 298Combined sources
Helixi33 – 4210Combined sources
Beta strandi47 – 537Combined sources
Helixi54 – 563Combined sources
Beta strandi60 – 689Combined sources
Beta strandi71 – 8010Combined sources
Helixi84 – 9613Combined sources
Beta strandi98 – 1069Combined sources
Helixi110 – 11910Combined sources
Beta strandi124 – 13310Combined sources
Beta strandi136 – 1449Combined sources
Beta strandi150 – 15910Combined sources
Beta strandi161 – 1644Combined sources
Beta strandi184 – 1918Combined sources
Helixi197 – 20812Combined sources
Helixi219 – 2224Combined sources
Turni223 – 2264Combined sources
Beta strandi227 – 2348Combined sources
Beta strandi240 – 2478Combined sources
Beta strandi250 – 2523Combined sources
Helixi255 – 2639Combined sources
Beta strandi267 – 2748Combined sources
Helixi278 – 28710Combined sources
Helixi297 – 2993Combined sources
Helixi303 – 3075Combined sources
Helixi314 – 3207Combined sources
Beta strandi323 – 3264Combined sources
Beta strandi331 – 3377Combined sources
Beta strandi340 – 3445Combined sources
Beta strandi347 – 3559Combined sources
Helixi363 – 37614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T47X-ray2.50A/B1-381[»]
ProteinModelPortaliQ53586.
SMRiQ53586. Positions 16-377.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53586.

Family & Domainsi

Sequence similaritiesi

Belongs to the 4HPPD family.Curated

Phylogenomic databases

eggNOGiENOG4105E6K. Bacteria.
COG3185. LUCA.
HOGENOMiHOG000188687.
KOiK00457.
OMAiAKQAAHY.
OrthoDBiEOG68SVVG.

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR005956. 4OHPhenylPyrv_dOase.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
[Graphical view]
PANTHERiPTHR11959. PTHR11959. 1 hit.
PfamiPF00903. Glyoxalase. 2 hits.
[Graphical view]
PIRSFiPIRSF009283. HPP_dOase. 1 hit.
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR01263. 4HPPD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTTHHTPD TARQADPFPV KGMDAVVFAV GNAKQAAHYY STAFGMQLVA
60 70 80 90 100
YSGPENGSRE TASYVLTNGS ARFVLTSVIK PATPWGHFLA DHVAEHGDGV
110 120 130 140 150
VDLAIEVPDA RAAHAYAIEH GARSVAEPYE LKDEHGTVVL AAIATYGKTR
160 170 180 190 200
HTLVDRTGYD GPYLPGYVAA APIVEPPAHR TFQAIDHCVG NVELGRMNEW
210 220 230 240 250
VGFYNKVMGF TNMKEFVGDD IATEYSALMS KVVADGTLKV KFPINEPALA
260 270 280 290 300
KKKSQIDEYL EFYGGAGVQH IALNTGDIVE TVRTMRAAGV QFLDTPDSYY
310 320 330 340 350
DTLGEWVGDT RVPVDTLREL KILADRDEDG YLLQIFTKPV QDRPTVFFEI
360 370 380
IERHGSMGFG KGNFKALFEA IEREQEKRGN L
Length:381
Mass (Da):41,863
Last modified:May 9, 2003 - v2
Checksum:i4A3B3E7B646165D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401Missing in AAA50231 (PubMed:8071207).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11864 Genomic DNA. Translation: AAA50231.1.
AB070935 Genomic DNA. Translation: BAB69150.1.
BA000030 Genomic DNA. Translation: BAC72861.1.
RefSeqiWP_010986553.1. NZ_JZJK01000072.1.

Genome annotation databases

EnsemblBacteriaiBAC72861; BAC72861; SAV_5149.
KEGGisma:SAV_5149.
PATRICi23724242. VBIStrAve112782_5488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11864 Genomic DNA. Translation: AAA50231.1.
AB070935 Genomic DNA. Translation: BAB69150.1.
BA000030 Genomic DNA. Translation: BAC72861.1.
RefSeqiWP_010986553.1. NZ_JZJK01000072.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T47X-ray2.50A/B1-381[»]
ProteinModelPortaliQ53586.
SMRiQ53586. Positions 16-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_5149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC72861; BAC72861; SAV_5149.
KEGGisma:SAV_5149.
PATRICi23724242. VBIStrAve112782_5488.

Phylogenomic databases

eggNOGiENOG4105E6K. Bacteria.
COG3185. LUCA.
HOGENOMiHOG000188687.
KOiK00457.
OMAiAKQAAHY.
OrthoDBiEOG68SVVG.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00362.
BioCyciSAVE227882:GJU1-5215-MONOMER.
BRENDAi1.13.11.27. 5980.
SABIO-RKQ53586.

Miscellaneous databases

EvolutionaryTraceiQ53586.

Family and domain databases

Gene3Di3.10.180.10. 2 hits.
InterProiIPR005956. 4OHPhenylPyrv_dOase.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
[Graphical view]
PANTHERiPTHR11959. PTHR11959. 1 hit.
PfamiPF00903. Glyoxalase. 2 hits.
[Graphical view]
PIRSFiPIRSF009283. HPP_dOase. 1 hit.
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR01263. 4HPPD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A Streptomyces avermitilis gene encoding a 4-hydroxyphenylpyruvic acid dioxygenase-like protein that directs the production of homogentisic acid and an ochronotic pigment in Escherichia coli."
    Denoya C.D., Skinner D.D., Morgenstern M.R.
    J. Bacteriol. 176:5312-5319(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 31272 / MA-4848.
  2. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
    Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680.
  3. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
    Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
    Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680.
  4. "Structure of the ferrous form of (4-hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis in complex with the therapeutic herbicide, NTBC."
    Brownlee J.M., Johnson-Winters K., Harrison D.H.T., Moran G.R.
    Biochemistry 43:6370-6377(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), METAL-BINDING SITES, SUBUNIT.

Entry informationi

Entry nameiHPPD_STRAW
AccessioniPrimary (citable) accession number: Q53586
Secondary accession number(s): Q93HN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 9, 2003
Last modified: November 11, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.