Reviewed, UniProtKB/Swiss-Prot Q53586 (HPPD_STRAW)

Names and origin

Protein names

Recommended name:
    4-hydroxyphenylpyruvate dioxygenase
      Short name=HPPDase
      Short name=4HPPD
      Short name=HPD
    EC=1.13.11.27

Gene names

Name:	hpd
Ordered Locus Names:	SAV_5149

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 3/6.
Subunit structure	Homodimer. Ref.4
Sequence similarities	Belongs to the 4HPPD family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 381

381

4-hydroxyphenylpyruvate dioxygenase

PRO_0000088409

Sites

Metal binding

187

1

Iron

Metal binding

270

1

Iron

Metal binding

349

1

Iron

Experimental info

Sequence conflict

40

1

Missing in AAA50231. Ref.1

Secondary structure

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381

Helix Strand Turn

Details...

Beta strand

22 – 29

8

Helix

33 – 42

10

Beta strand

47 – 53

7

Helix

54 – 56

3

Beta strand

60 – 68

9

Beta strand

71 – 80

10

Helix

84 – 96

13

Beta strand

98 – 106

9

Helix

110 – 119

10

Beta strand

124 – 133

10

Beta strand

136 – 144

9

Beta strand

150 – 159

10

Beta strand

161 – 164

4

Beta strand

184 – 191

8

Helix

197 – 208

12

Helix

219 – 222

4

Turn

223 – 226

4

Beta strand

227 – 234

8

Beta strand

240 – 247

8

Beta strand

250 – 252

3

Helix

255 – 263

9

Beta strand

267 – 274

8

Helix

278 – 287

10

Helix

297 – 299

3

Helix

303 – 307

5

Helix

314 – 320

7

Beta strand

323 – 326

4

Beta strand

331 – 337

7

Beta strand

340 – 344

5

Beta strand

347 – 355

9

Helix

363 – 376

14

Sequences

	Sequence		Length	Mass (Da)
	Q53586-1 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 4A3B3E7B646165D7	FASTA	381	41,863
	10 20 30 40 50 60 MTQTTHHTPD TARQADPFPV KGMDAVVFAV GNAKQAAHYY STAFGMQLVA YSGPENGSRE 70 80 90 100 110 120 TASYVLTNGS ARFVLTSVIK PATPWGHFLA DHVAEHGDGV VDLAIEVPDA RAAHAYAIEH 130 140 150 160 170 180 GARSVAEPYE LKDEHGTVVL AAIATYGKTR HTLVDRTGYD GPYLPGYVAA APIVEPPAHR 190 200 210 220 230 240 TFQAIDHCVG NVELGRMNEW VGFYNKVMGF TNMKEFVGDD IATEYSALMS KVVADGTLKV 250 260 270 280 290 300 KFPINEPALA KKKSQIDEYL EFYGGAGVQH IALNTGDIVE TVRTMRAAGV QFLDTPDSYY 310 320 330 340 350 360 DTLGEWVGDT RVPVDTLREL KILADRDEDG YLLQIFTKPV QDRPTVFFEI IERHGSMGFG 370 380 KGNFKALFEA IEREQEKRGN L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Streptomyces avermitilis gene encoding a 4-hydroxyphenylpyruvic acid dioxygenase-like protein that directs the production of homogentisic acid and an ochronotic pigment in Escherichia coli." Denoya C.D., Skinner D.D., Morgenstern M.R. J. Bacteriol. 176:5312-5319(1994) [PubMed: 8071207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31272 / MA-4848.
[2]	"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.
[3]	"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.
[4]	"Structure of the ferrous form of (4-hydroxyphenyl)pyruvate dioxygenase from Streptomyces avermitilis in complex with the therapeutic herbicide, NTBC." Brownlee J.M., Johnson-Winters K., Harrison D.H.T., Moran G.R. Biochemistry 43:6370-6377(2004) [PubMed: 15157070] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), METAL-BINDING SITES, SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U11864 Genomic DNA. Translation: AAA50231.1.
AB070935 Genomic DNA. Translation: BAB69150.1.
BA000030 Genomic DNA. Translation: BAC72861.1.

RefSeq

NP_826326.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T47	X-ray	2.50	A/B	1-381	[»]

ModBase

Search...

Genome annotation databases

GeneID

1211215.

GenomeReviews

Gene locus SAV_5149 in contig BA000030_GR.

NMPDR

fig|227882.1.peg.5152.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q53586.

OMA

Q53586. VQDRPTV.

Enzyme and pathway databases

BioCyc

SAVE227882:SAV5149-MON.

BRENDA

1.13.11.27. 140873.

Family and domain databases

InterPro

IPR005956. 4OHPhenylPyrv_dOase.
IPR004360. Glyas_bleo-R_dOase.
[Graphical view]

PANTHER

PTHR11959. HPP_dOase. 1 hit.

Pfam

PF00903. Glyoxalase. 2 hits.
[Graphical view]

PIRSF

PIRSF009283. HPP_dOase. 1 hit.

TIGRFAMs

TIGR01263. 4HPPD. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HPPD_STRAW

Accession

Primary (citable) accession number: Q53586
Secondary accession number(s): Q93HN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 9, 2003
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)