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Q53563

- MMOC_METTR

UniProt

Q53563 - MMOC_METTR

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Protein
Methane monooxygenase component C
Gene
mmoC
Organism
Methylosinus trichosporium
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds. The component C is the iron-sulfur flavoprotein of sMMO.

Catalytic activityi

Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

Cofactori

Binds 1 2Fe-2S cluster.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Iron-sulfur (2Fe-2S)
Metal bindingi41 – 411Iron-sulfur (2Fe-2S)
Metal bindingi44 – 441Iron-sulfur (2Fe-2S)
Metal bindingi76 – 761Iron-sulfur (2Fe-2S)

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi215 – 22915FAD By similarity
Add
BLAST

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. methane monooxygenase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. one-carbon metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Electron transport, One-carbon metabolism, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3870.

Names & Taxonomyi

Protein namesi
Recommended name:
Methane monooxygenase component C (EC:1.14.13.25)
Alternative name(s):
Methane hydroxylase
Methane monooxygenase reductase
Short name:
MMOR
Gene namesi
Name:mmoC
OrganismiMethylosinus trichosporium
Taxonomic identifieri426 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylocystaceaeMethylosinus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Methane monooxygenase component C
PRO_0000189409Add
BLAST

Interactioni

Subunit structurei

The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD).

Structurei

3D structure databases

ProteinModelPortaliQ53563.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 92922Fe-2S ferredoxin-type
Add
BLAST
Domaini101 – 205105FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR001221. Phe_hydroxylase.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00371. FPNCR.
PR00410. PHEHYDRXLASE.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q53563-1 [UniParc]FASTAAdd to Basket

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MYQIVIETED GETCRRMRPS EDWISRAEAE RNLLASCRAG CATCKADCTD    50
GDYELIDVKV QAVPPDEEED GKVLLCRTFP RSDLHLLVPY TYDRISFEAI 100
QTNWLAEILA CDRVSSNVVR LVLQRSRPMA ARISLNFVPG QFVDIEIPGT 150
HTRRSYSMAS VAEDGQLEFI IRLLPDGAFS KFLQTEAKVG MRVDLRGPAG 200
SFFLHDHGGR SRVFVAGGTG LSPVLSMIRQ LGKASDPSPA TLLFGVTNRE 250
ELFYVDELKT LAQSMPTLGV RIAVVNDDGG NGVDKGTVID LLRAELEIDL 300
LLGHARRRRR RETARSCRED HRDRCPAWRS DFLEKFLASG 340
Length:340
Mass (Da):37,991
Last modified:November 1, 1996 - v1
Checksum:i79AE21DA8079E6D8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S81887 Genomic DNA. Translation: AAB21393.1.
PIRiC48360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S81887 Genomic DNA. Translation: AAB21393.1 .
PIRi C48360.

3D structure databases

ProteinModelPortali Q53563.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-3870.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR001221. Phe_hydroxylase.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PRINTSi PR00371. FPNCR.
PR00410. PHEHYDRXLASE.
SUPFAMi SSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The methane monooxygenase gene cluster of Methylosinus trichosporium: cloning and sequencing of the mmoC gene."
    Cardy D.L.N., Laidler V., Salmond G.P.C., Murrell J.C.
    Arch. Microbiol. 156:477-483(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OB3b.
  2. "Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b. Identification of sites of component interaction."
    Fox B.G., Liu Y., Dege J.E., Lipscomb J.D.
    J. Biol. Chem. 266:540-550(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-13, CHARACTERIZATION, COMPLEX FORMATION.
    Strain: OB3b.

Entry informationi

Entry nameiMMOC_METTR
AccessioniPrimary (citable) accession number: Q53563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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