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Q53560 (DHLE_BACLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Leucine dehydrogenase
Short name=LeuDH
EC=1.4.1.9
Gene names
Name:ldh
OrganismBacillus licheniformis
Taxonomic identifier1402 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase By similarity.

Catalytic activity

L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH.

Pathway

Amino-acid degradation; L-leucine degradation; 4-methyl-2-oxopentanoate from L-leucine (dehydrogenase route): step 1/1.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   Biological processBranched-chain amino acid catabolism
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processleucine catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionleucine dehydrogenase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 364364Leucine dehydrogenase
PRO_0000182802

Regions

Nucleotide binding180 – 1867NAD Potential

Sites

Active site801 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q53560 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A13A967F6E84F873

FASTA36440,040
        10         20         30         40         50         60 
MELFRYMEQY DYEQLVFCQD KQSGLKAIIA IHDTTLGPAL GGTRMWTYES EEAAIEDALR 

        70         80         90        100        110        120 
LARGMTYKNA AAGLNLGGGK TVIIGDPRKD KNEEMFRAFG RYIQGLNGRY ITAEDVGTTV 

       130        140        150        160        170        180 
EDMDIIHDET DFVTGISPAF GSSGNPSPVT AYGVYKGMKA AAKAAFGTDS LEGKTVAVQG 

       190        200        210        220        230        240 
VGNVAYNLCR HLHEEGAKLI VTDINKEAVE RAVAEFGARA VDPDDIYSQE CDIYAPCALG 

       250        260        270        280        290        300 
ATINDDTIPQ LKAKVIAGAA NNQLKETRHG DQIHDMGIVY APDYVINAGG VINVADELYG 

       310        320        330        340        350        360 
YNSERALKKV EGIYGNIERV LEISKRDRIP TYLAADRLAE ERIERMRQSR SQFLQNGHHI 


LSRR

« Hide

References

[1]"Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9."
Nagata S., Bakthavatsalam S., Galkin A.G., Asada H., Sakai S., Esaki N., Soda K., Ohshima T., Nagasaki S., Misono H.
Appl. Microbiol. Biotechnol. 44:432-438(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: TSN9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S81735 Genomic DNA. Translation: AAB36205.1.

3D structure databases

ProteinModelPortalQ53560.
SMRQ53560. Positions 1-364.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00363; UER00858.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016211. Glu/Phe/Leu/Val_DH_bac/arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11606:SF3. PTHR11606:SF3. 1 hit.
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000188. Phe_leu_dh. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHLE_BACLI
AccessionPrimary (citable) accession number: Q53560
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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