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Protein

Leucine dehydrogenase

Gene

ldh

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible deamination of L-leucine to 4-methyl-2-oxopentanoate. Can also use other substrates such as L-isoleucine, L-valine and L-2-aminobutyrate. The 2-oxo analogs of branched-chain and straight-chain amino acids serve as good substrates for the reverse reaction.1 Publication

Catalytic activityi

L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH.1 Publication

Kineticsi

  1. KM=2.1 mM for L-leucine1 Publication
  2. KM=3.3 mM for L-isoleucine1 Publication
  3. KM=12.5 mM for L-valine1 Publication
  4. KM=0.31 mM for NAD+1 Publication
  5. KM=1.0 mM for 4-methyl-2-oxopentanoate1 Publication
  6. KM=3.8 mM for 2-oxoisovalerate1 Publication
  7. KM=2.4 mM for 2-oxovalerate1 Publication
  8. KM=0.25 mM for NADH1 Publication
  9. KM=330 mM for NH31 Publication

pH dependencei

Optimum pH is 10.3 for the oxidative deamination of L-leucine.1 Publication

Temperature dependencei

Thermostable. Retains full activity on heating at 65 degrees Celsius for 1 hour, but loses the activity completely on heating at 70 degrees Celsius for 1 hour.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi180 – 1867NADSequence Analysis

GO - Molecular functioni

  1. leucine dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. leucine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Branched-chain amino acid catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00363; UER00858.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine dehydrogenase (EC:1.4.1.9)
Short name:
LeuDH
Gene namesi
Name:ldh
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Leucine dehydrogenasePRO_0000182802Add
BLAST

Interactioni

Subunit structurei

Homooctamer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ53560.
SMRiQ53560. Positions 1-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016211. Glu/Phe/Leu/Val_DH_bac/arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11606:SF3. PTHR11606:SF3. 1 hit.
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000188. Phe_leu_dh. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q53560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELFRYMEQY DYEQLVFCQD KQSGLKAIIA IHDTTLGPAL GGTRMWTYES
60 70 80 90 100
EEAAIEDALR LARGMTYKNA AAGLNLGGGK TVIIGDPRKD KNEEMFRAFG
110 120 130 140 150
RYIQGLNGRY ITAEDVGTTV EDMDIIHDET DFVTGISPAF GSSGNPSPVT
160 170 180 190 200
AYGVYKGMKA AAKAAFGTDS LEGKTVAVQG VGNVAYNLCR HLHEEGAKLI
210 220 230 240 250
VTDINKEAVE RAVAEFGARA VDPDDIYSQE CDIYAPCALG ATINDDTIPQ
260 270 280 290 300
LKAKVIAGAA NNQLKETRHG DQIHDMGIVY APDYVINAGG VINVADELYG
310 320 330 340 350
YNSERALKKV EGIYGNIERV LEISKRDRIP TYLAADRLAE ERIERMRQSR
360
SQFLQNGHHI LSRR
Length:364
Mass (Da):40,040
Last modified:November 1, 1996 - v1
Checksum:iA13A967F6E84F873
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81735 Genomic DNA. Translation: AAB36205.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81735 Genomic DNA. Translation: AAB36205.1.

3D structure databases

ProteinModelPortaliQ53560.
SMRiQ53560. Positions 1-364.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00363; UER00858.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016211. Glu/Phe/Leu/Val_DH_bac/arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11606:SF3. PTHR11606:SF3. 1 hit.
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000188. Phe_leu_dh. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9."
    Nagata S., Bakthavatsalam S., Galkin A.G., Asada H., Sakai S., Esaki N., Soda K., Ohshima T., Nagasaki S., Misono H.
    Appl. Microbiol. Biotechnol. 44:432-438(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, SUBUNIT.
    Strain: TSN9.

Entry informationi

Entry nameiDHLE_BACLI
AccessioniPrimary (citable) accession number: Q53560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.