Reviewed, UniProtKB/Swiss-Prot Q53560 (DHLE_BACLI)

Names and origin

Protein names

Recommended name:
    Leucine dehydrogenase
      Short name=LeuDH
    EC=1.4.1.9

Gene names

Name:

ldh

Organism

Bacillus licheniformis

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase By similarity.
Catalytic activity	L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH.
Pathway	Amino-acid degradation; L-leucine degradation; 4-methyl-2-oxopentanoate from L-leucine (dehydrogenase route): step 1/1.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Biological process	Branched-chain amino acid catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	branched chain family amino acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro leucine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Leucine dehydrogenase		PRO_0000182802
Regions
	Nucleotide binding	180 – 186	7	NAD Potential
Sites
	Active site	80	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q53560-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A13A967F6E84F873	FASTA	364	40,040
	10 20 30 40 50 60 MELFRYMEQY DYEQLVFCQD KQSGLKAIIA IHDTTLGPAL GGTRMWTYES EEAAIEDALR 70 80 90 100 110 120 LARGMTYKNA AAGLNLGGGK TVIIGDPRKD KNEEMFRAFG RYIQGLNGRY ITAEDVGTTV 130 140 150 160 170 180 EDMDIIHDET DFVTGISPAF GSSGNPSPVT AYGVYKGMKA AAKAAFGTDS LEGKTVAVQG 190 200 210 220 230 240 VGNVAYNLCR HLHEEGAKLI VTDINKEAVE RAVAEFGARA VDPDDIYSQE CDIYAPCALG 250 260 270 280 290 300 ATINDDTIPQ LKAKVIAGAA NNQLKETRHG DQIHDMGIVY APDYVINAGG VINVADELYG 310 320 330 340 350 360 YNSERALKKV EGIYGNIERV LEISKRDRIP TYLAADRLAE ERIERMRQSR SQFLQNGHHI LSRR

References

[1]

"Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9." Nagata S., Bakthavatsalam S., Galkin A.G., Asada H., Sakai S., Esaki N., Soda K., Ohshima T., Nagasaki S., Misono H. Appl. Microbiol. Biotechnol. 44:432-438(1995) [PubMed: 8597545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TSN9.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S81735 Genomic DNA. Translation: AAB36205.1.
3D structure databases
HSSP	HSSP built from PDB template 1C1D based on UniProtKB Q59771.
SMR	Q53560. Positions 1-364.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.9. 1017.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR016211. Glu/Phe/Leu/Val_DH_bac/arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000188. Phe_leu_dh. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHLE_BACLI

Accession

Primary (citable) accession number: Q53560

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)