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Protein

Leucine dehydrogenase

Gene

ldh

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible deamination of L-leucine to 4-methyl-2-oxopentanoate. Can also use other substrates such as L-isoleucine, L-valine and L-2-aminobutyrate. The 2-oxo analogs of branched-chain and straight-chain amino acids serve as good substrates for the reverse reaction.1 Publication

Catalytic activityi

L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH.1 Publication

Kineticsi

  1. KM=2.1 mM for L-leucine1 Publication
  2. KM=3.3 mM for L-isoleucine1 Publication
  3. KM=12.5 mM for L-valine1 Publication
  4. KM=0.31 mM for NAD+1 Publication
  5. KM=1.0 mM for 4-methyl-2-oxopentanoate1 Publication
  6. KM=3.8 mM for 2-oxoisovalerate1 Publication
  7. KM=2.4 mM for 2-oxovalerate1 Publication
  8. KM=0.25 mM for NADH1 Publication
  9. KM=330 mM for NH31 Publication

    pH dependencei

    Optimum pH is 10.3 for the oxidative deamination of L-leucine.1 Publication

    Temperature dependencei

    Thermostable. Retains full activity on heating at 65 degrees Celsius for 1 hour, but loses the activity completely on heating at 70 degrees Celsius for 1 hour.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei80 – 801PROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi180 – 1867NADSequence Analysis

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Branched-chain amino acid catabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00363; UER00858.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine dehydrogenase (EC:1.4.1.9)
    Short name:
    LeuDH
    Gene namesi
    Name:ldh
    OrganismiBacillus licheniformis
    Taxonomic identifieri1402 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364Leucine dehydrogenasePRO_0000182802Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ53560.
    SMRiQ53560. Positions 1-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR016211. Glu/Phe/Leu/Val_DH_bac/arc.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11606:SF3. PTHR11606:SF3. 1 hit.
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000188. Phe_leu_dh. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q53560-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MELFRYMEQY DYEQLVFCQD KQSGLKAIIA IHDTTLGPAL GGTRMWTYES
    60 70 80 90 100
    EEAAIEDALR LARGMTYKNA AAGLNLGGGK TVIIGDPRKD KNEEMFRAFG
    110 120 130 140 150
    RYIQGLNGRY ITAEDVGTTV EDMDIIHDET DFVTGISPAF GSSGNPSPVT
    160 170 180 190 200
    AYGVYKGMKA AAKAAFGTDS LEGKTVAVQG VGNVAYNLCR HLHEEGAKLI
    210 220 230 240 250
    VTDINKEAVE RAVAEFGARA VDPDDIYSQE CDIYAPCALG ATINDDTIPQ
    260 270 280 290 300
    LKAKVIAGAA NNQLKETRHG DQIHDMGIVY APDYVINAGG VINVADELYG
    310 320 330 340 350
    YNSERALKKV EGIYGNIERV LEISKRDRIP TYLAADRLAE ERIERMRQSR
    360
    SQFLQNGHHI LSRR
    Length:364
    Mass (Da):40,040
    Last modified:November 1, 1996 - v1
    Checksum:iA13A967F6E84F873
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    S81735 Genomic DNA. Translation: AAB36205.1.
    RefSeqiWP_003183340.1. NZ_JFYM01000001.1.

    Genome annotation databases

    GeneIDi3027388.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    S81735 Genomic DNA. Translation: AAB36205.1.
    RefSeqiWP_003183340.1. NZ_JFYM01000001.1.

    3D structure databases

    ProteinModelPortaliQ53560.
    SMRiQ53560. Positions 1-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi3027388.

    Enzyme and pathway databases

    UniPathwayiUPA00363; UER00858.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR016211. Glu/Phe/Leu/Val_DH_bac/arc.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11606:SF3. PTHR11606:SF3. 1 hit.
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000188. Phe_leu_dh. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9."
      Nagata S., Bakthavatsalam S., Galkin A.G., Asada H., Sakai S., Esaki N., Soda K., Ohshima T., Nagasaki S., Misono H.
      Appl. Microbiol. Biotechnol. 44:432-438(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, SUBUNIT.
      Strain: TSN9.

    Entry informationi

    Entry nameiDHLE_BACLI
    AccessioniPrimary (citable) accession number: Q53560
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: May 27, 2015
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.