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Protein

Citrate synthase

Gene

gltA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. no protein annotated in this organism
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei263PROSITE-ProRule annotation1
Active sitei313PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BRENDAi2.3.3.16. 5243.
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:PF0203
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001699762 – 377Citrate synthaseAdd BLAST376

Proteomic databases

PRIDEiQ53554.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0203.

Structurei

Secondary structure

1377
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi15 – 25Combined sources11
Turni26 – 29Combined sources4
Beta strandi30 – 33Combined sources4
Helixi38 – 44Combined sources7
Helixi47 – 56Combined sources10
Helixi62 – 73Combined sources12
Helixi80 – 88Combined sources9
Helixi95 – 109Combined sources15
Turni111 – 114Combined sources4
Helixi119 – 144Combined sources26
Helixi158 – 167Combined sources10
Helixi173 – 186Combined sources14
Helixi193 – 202Combined sources10
Turni203 – 205Combined sources3
Helixi208 – 220Combined sources13
Turni222 – 226Combined sources5
Helixi227 – 238Combined sources12
Helixi241 – 243Combined sources3
Helixi244 – 254Combined sources11
Helixi271 – 283Combined sources13
Helixi286 – 302Combined sources17
Turni303 – 307Combined sources5
Turni312 – 315Combined sources4
Helixi316 – 321Combined sources6
Turni322 – 324Combined sources3
Helixi327 – 329Combined sources3
Helixi330 – 350Combined sources21
Beta strandi359 – 362Combined sources4
Helixi373 – 375Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJ8X-ray1.90A/B7-377[»]
ProteinModelPortaliQ53554.
SMRiQ53554.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53554.

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiarCOG04237. Archaea.
COG0372. LUCA.
HOGENOMiHOG000021225.
KOiK01647.
OMAiEDPRATH.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTEKYLAKG LEDVYIDQTN ICYIDGKEGK LYYRGYSVEE LAELSTFEEV
60 70 80 90 100
VYLLWWGKLP SLSELENFKK ELAKSRGLPK EVIEIMEALP KNTHPMGALR
110 120 130 140 150
TIISYLGNID DSGDIPVTPE EVYRIGISVT AKIPTIVANW YRIKNGLEYV
160 170 180 190 200
PPKEKLSHAA NFLYMLHGEE PPKEWEKAMD VALILYAEHE INASTLAVMT
210 220 230 240 250
VGSTLSDYYS AILAGIGALK GPIHGGAVEE AIKQFMEIGS PEKVEEWFFK
260 270 280 290 300
ALQQKRKIMG AGHRVYKTYD PRARIFKKYA SKLGDKKLFE IAERLERLVE
310 320 330 340 350
EYLSKKGISI NVDYWSGLVF YGMKIPIELY TTIFAMGRIA GWTAHLAEYV
360 370
SHNRIIRPRL QYVGEIGKKY LPIELRR
Length:377
Mass (Da):43,050
Last modified:January 23, 2007 - v2
Checksum:iA73E70EF123BE44B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81109 Genomic DNA. Translation: AAB35835.2.
AE009950 Genomic DNA. Translation: AAL80327.1.
RefSeqiWP_011011316.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80327; AAL80327; PF0203.
GeneIDi1468035.
KEGGipfu:PF0203.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81109 Genomic DNA. Translation: AAB35835.2.
AE009950 Genomic DNA. Translation: AAL80327.1.
RefSeqiWP_011011316.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJ8X-ray1.90A/B7-377[»]
ProteinModelPortaliQ53554.
SMRiQ53554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0203.

Proteomic databases

PRIDEiQ53554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80327; AAL80327; PF0203.
GeneIDi1468035.
KEGGipfu:PF0203.

Phylogenomic databases

eggNOGiarCOG04237. Archaea.
COG0372. LUCA.
HOGENOMiHOG000021225.
KOiK01647.
OMAiEDPRATH.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
BRENDAi2.3.3.16. 5243.

Miscellaneous databases

EvolutionaryTraceiQ53554.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCISY_PYRFU
AccessioniPrimary (citable) accession number: Q53554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.