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Q53554

- CISY_PYRFU

UniProt

Q53554 - CISY_PYRFU

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Protein
Citrate synthase
Gene
gltA, PF0203
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei263 – 2631 By similarity
Active sitei313 – 3131 By similarity

GO - Molecular functioni

  1. transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer Source: InterPro

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:PF0203
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 377376Citrate synthase
PRO_0000169976Add
BLAST

Proteomic databases

PRIDEiQ53554.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0203.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113
Beta strandi15 – 2511
Turni26 – 294
Beta strandi30 – 334
Helixi38 – 447
Helixi47 – 5610
Helixi62 – 7312
Helixi80 – 889
Helixi95 – 10915
Turni111 – 1144
Helixi119 – 14426
Helixi158 – 16710
Helixi173 – 18614
Helixi193 – 20210
Turni203 – 2053
Helixi208 – 22013
Turni222 – 2265
Helixi227 – 23812
Helixi241 – 2433
Helixi244 – 25411
Helixi271 – 28313
Helixi286 – 30217
Turni303 – 3075
Turni312 – 3154
Helixi316 – 3216
Turni322 – 3243
Helixi327 – 3293
Helixi330 – 35021
Beta strandi359 – 3624
Helixi373 – 3753

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AJ8X-ray1.90A/B7-377[»]
ProteinModelPortaliQ53554.
SMRiQ53554. Positions 7-377.

Miscellaneous databases

EvolutionaryTraceiQ53554.

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021225.
KOiK01647.
OMAiKVANPYP.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53554-1 [UniParc]FASTAAdd to Basket

« Hide

MNTEKYLAKG LEDVYIDQTN ICYIDGKEGK LYYRGYSVEE LAELSTFEEV    50
VYLLWWGKLP SLSELENFKK ELAKSRGLPK EVIEIMEALP KNTHPMGALR 100
TIISYLGNID DSGDIPVTPE EVYRIGISVT AKIPTIVANW YRIKNGLEYV 150
PPKEKLSHAA NFLYMLHGEE PPKEWEKAMD VALILYAEHE INASTLAVMT 200
VGSTLSDYYS AILAGIGALK GPIHGGAVEE AIKQFMEIGS PEKVEEWFFK 250
ALQQKRKIMG AGHRVYKTYD PRARIFKKYA SKLGDKKLFE IAERLERLVE 300
EYLSKKGISI NVDYWSGLVF YGMKIPIELY TTIFAMGRIA GWTAHLAEYV 350
SHNRIIRPRL QYVGEIGKKY LPIELRR 377
Length:377
Mass (Da):43,050
Last modified:January 23, 2007 - v2
Checksum:iA73E70EF123BE44B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S81109 Genomic DNA. Translation: AAB35835.2.
AE009950 Genomic DNA. Translation: AAL80327.1.
RefSeqiNP_577932.1. NC_003413.1.
WP_011011316.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80327; AAL80327; PF0203.
GeneIDi1468035.
KEGGipfu:PF0203.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S81109 Genomic DNA. Translation: AAB35835.2 .
AE009950 Genomic DNA. Translation: AAL80327.1 .
RefSeqi NP_577932.1. NC_003413.1.
WP_011011316.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AJ8 X-ray 1.90 A/B 7-377 [» ]
ProteinModelPortali Q53554.
SMRi Q53554. Positions 7-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF0203.

Proteomic databases

PRIDEi Q53554.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL80327 ; AAL80327 ; PF0203 .
GeneIDi 1468035.
KEGGi pfu:PF0203.

Phylogenomic databases

eggNOGi COG0372.
HOGENOMi HOG000021225.
KOi K01647.
OMAi KVANPYP.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER00717 .

Miscellaneous databases

EvolutionaryTracei Q53554.

Family and domain databases

Gene3Di 1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProi IPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view ]
PANTHERi PTHR11739. PTHR11739. 1 hit.
Pfami PF00285. Citrate_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF001369. Citrate_synth. 1 hit.
PRINTSi PR00143. CITRTSNTHASE.
SUPFAMi SSF48256. SSF48256. 1 hit.
TIGRFAMsi TIGR01800. cit_synth_II. 1 hit.
PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Citrate synthase from the hyperthermophilic Archaeon, Pyrococcus furiosus."
    Muir J.M., Russell R.J., Hough D.W., Danson M.J.
    Protein Eng. 8:583-592(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "The crystal structure of citrate synthase from the hyperthermophilic archaeon Pyrococcus furiosus at 1.9-A resolution."
    Russell R.J., Ferguson J.M., Hough D.W., Danson M.J., Taylor G.L.
    Biochemistry 36:9983-9994(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiCISY_PYRFU
AccessioniPrimary (citable) accession number: Q53554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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