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Q53554 (CISY_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Citrate synthase
EC=2.3.3.1
Gene names
Name:gltA
Ordered Locus Names:PF0203
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus ›

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the citrate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 377376Citrate synthase
PRO_0000169976

Sites

Active site2631 By similarity
Active site3131 By similarity

Secondary structure

1 .................................................... 377
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q53554 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: A73E70EF123BE44B

FASTA37743,050
        10         20         30         40         50         60 
MNTEKYLAKG LEDVYIDQTN ICYIDGKEGK LYYRGYSVEE LAELSTFEEV VYLLWWGKLP 

        70         80         90        100        110        120 
SLSELENFKK ELAKSRGLPK EVIEIMEALP KNTHPMGALR TIISYLGNID DSGDIPVTPE 

       130        140        150        160        170        180 
EVYRIGISVT AKIPTIVANW YRIKNGLEYV PPKEKLSHAA NFLYMLHGEE PPKEWEKAMD 

       190        200        210        220        230        240 
VALILYAEHE INASTLAVMT VGSTLSDYYS AILAGIGALK GPIHGGAVEE AIKQFMEIGS 

       250        260        270        280        290        300 
PEKVEEWFFK ALQQKRKIMG AGHRVYKTYD PRARIFKKYA SKLGDKKLFE IAERLERLVE 

       310        320        330        340        350        360 
EYLSKKGISI NVDYWSGLVF YGMKIPIELY TTIFAMGRIA GWTAHLAEYV SHNRIIRPRL 

       370 
QYVGEIGKKY LPIELRR

« Hide

References

« Hide 'large scale' references
[1]"Citrate synthase from the hyperthermophilic Archaeon, Pyrococcus furiosus."
Muir J.M., Russell R.J., Hough D.W., Danson M.J.
Protein Eng. 8:583-592(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]"The crystal structure of citrate synthase from the hyperthermophilic archaeon Pyrococcus furiosus at 1.9-A resolution."
Russell R.J., Ferguson J.M., Hough D.W., Danson M.J., Taylor G.L.
Biochemistry 36:9983-9994(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S81109 Genomic DNA. Translation: AAB35835.2.
AE009950 Genomic DNA. Translation: AAL80327.1.
RefSeqNP_577932.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AJ8X-ray1.90A/B7-377[»]
ProteinModelPortalQ53554.
SMRQ53554. Positions 7-377.
ModBaseSearch...

Protein-protein interaction databases

STRING186497.PF0203.

Proteomic databases

PRIDEQ53554.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL80327; AAL80327; PF0203.
GeneID1468035.
KEGGpfu:PF0203.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021225.
KOK01647.
OMAPGFMATG.
ProtClustDBCLSK803973.

Enzyme and pathway databases

UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR011278. 2-MeCitrate/Citrate_synth_I.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. Citrate_synthase_core. 1 hit.
TIGRFAMsTIGR01800. cit_synth_II. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ53554.

Entry information

Entry nameCISY_PYRFU
AccessionPrimary (citable) accession number: Q53554
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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