Q53552 (NHG2_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Salicylate hydroxylase
EC=1.14.13.1

Alternative name(s):
Salicylate 1-monooxygenase

Gene names

Name:

nahG

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Salicylate + NADH + O2 = catechol + NAD+ + H2O + CO2.
Cofactor	FAD.
Pathway	Aromatic compound metabolism; naphthalene degradation.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein NAD
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	salicylate 1-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Salicylate hydroxylase		PRO_0000096804
Regions
	Nucleotide binding	12 – 41	30	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q53552 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7E8017520726DD8C	FASTA	435	47,914
	10 20 30 40 50 60 MNDMNAKKPA LRVAIVGGGI SGLALALSLC KHSHLNVQLF EAAPAFGEVG AGVSFGPNAV 70 80 90 100 110 120 RAIVGLGLGQ AYFQVADRTP QPWEDIWFEW RRGSDASYLG ATIAGVGQSS VHRADFLDAL 130 140 150 160 170 180 VKHLPEGIAQ FRKRATQIEQ QGDELQVLFR DGTEYRCDLL IGRDGIKSAL RSYVLEGQGQ 190 200 210 220 230 240 DHLEPRFSGT CAYRGMVDSL QLREAYRING IDEHLVDVPQ MYLGLYGHIL TFPVRKGRIV 250 260 270 280 290 300 NVVAFTSDRS QPEPTWPADA PWVREASQRE MLDAFAGWGD ARALLECIPA PTLWALHDLP 310 320 330 340 350 360 ELPGYVHGRV ALIGDAAHAM LPHQGAGAGQ GLEDAYFLAR LLGDSRTETG NLPELLGAYD 370 380 390 400 410 420 DLRRPHACRV QRTTVETGEL YELRDPIVGA DEQLVGEILA TRFDWLWNHD LDADVAEARL 430 RMGWEAHEQI ALRQG

References

[1]	"Nucleotide sequence of salicylate hydroxylase gene and its 5'-flanking region of Pseudomonas putida KF715." Lee J., Oh J., Min K.R., Kim Y. Biochem. Biophys. Res. Commun. 218:544-548(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KF715.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S80995 Genomic DNA. Translation: AAB35960.1.
PIR	JC4590.
3D structure databases
ProteinModelPortal	Q53552.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00082.
Family and domain databases
InterPro	IPR002938. mOase_FAD-bd. IPR003042. Rng_hydrolase-like. IPR017631. Salicylate_mOase. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
TIGRFAMs	TIGR03219. salicylate_mono. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NHG2_PSEPU

Accession

Primary (citable) accession number: Q53552

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program