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Protein

Carboxylesterase 2

Gene

estB

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity.

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114Charge relay system1 Publication1
Active sitei168Charge relay system1 Publication1
Active sitei199Charge relay system1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

ESTHERipsefl-cxest. LYsophospholipase_carboxylesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxylesterase 2 (EC:3.1.1.1)
Alternative name(s):
Esterase II
Gene namesi
Name:estB
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022751 – 218Carboxylesterase 2Add BLAST218

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1218
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Beta strandi14 – 20Combined sources7
Turni27 – 30Combined sources4
Helixi31 – 38Combined sources8
Beta strandi44 – 48Combined sources5
Beta strandi53 – 55Combined sources3
Helixi57 – 59Combined sources3
Beta strandi63 – 65Combined sources3
Beta strandi70 – 72Combined sources3
Beta strandi74 – 76Combined sources3
Helixi81 – 100Combined sources20
Helixi105 – 107Combined sources3
Beta strandi108 – 113Combined sources6
Helixi115 – 125Combined sources11
Beta strandi134 – 139Combined sources6
Helixi153 – 156Combined sources4
Beta strandi160 – 165Combined sources6
Beta strandi169 – 171Combined sources3
Helixi173 – 184Combined sources12
Turni185 – 187Combined sources3
Beta strandi190 – 196Combined sources7
Beta strandi198 – 200Combined sources3
Helixi203 – 217Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUOX-ray1.80A/B1-218[»]
1AURX-ray2.50A/B1-218[»]
ProteinModelPortaliQ53547.
SMRiQ53547.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53547.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106CJB. Bacteria.
COG0400. LUCA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEPLILQPA KPADACVIWL HGLGADRYDF MPVAEALQES LLTTRFVLPQ
60 70 80 90 100
APTRPVTING GYEMPSWYDI KAMSPARSIS LEELEVSAKM VTDLIEAQKR
110 120 130 140 150
TGIDASRIFL AGFSQGGAVV FHTAFINWQG PLGGVIALST YAPTFGDELE
160 170 180 190 200
LSASQQRIPA LCLHGQYDDV VQNAMGRSAF EHLKSRGVTV TWQEYPMGHE
210
VLPQEIHDIG AWLAARLG
Length:218
Mass (Da):23,880
Last modified:November 1, 1996 - v1
Checksum:i6F1D4557410E4CB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79600 Genomic DNA. Translation: AAC60403.1.
PIRiJU0277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79600 Genomic DNA. Translation: AAC60403.1.
PIRiJU0277.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUOX-ray1.80A/B1-218[»]
1AURX-ray2.50A/B1-218[»]
ProteinModelPortaliQ53547.
SMRiQ53547.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERipsefl-cxest. LYsophospholipase_carboxylesterase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4106CJB. Bacteria.
COG0400. LUCA.

Miscellaneous databases

EvolutionaryTraceiQ53547.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEST2_PSEFL
AccessioniPrimary (citable) accession number: Q53547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.