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Protein

Carboxylesterase 2

Gene

estB

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity.

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141Charge relay system1 Publication
Active sitei168 – 1681Charge relay system1 Publication
Active sitei199 – 1991Charge relay system1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

ESTHERipsefl-cxest. LYsophospholipase_carboxylesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxylesterase 2 (EC:3.1.1.1)
Alternative name(s):
Esterase II
Gene namesi
Name:estB
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Carboxylesterase 2PRO_0000102275Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
218
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 73Combined sources
Beta strandi14 – 207Combined sources
Turni27 – 304Combined sources
Helixi31 – 388Combined sources
Beta strandi44 – 485Combined sources
Beta strandi53 – 553Combined sources
Helixi57 – 593Combined sources
Beta strandi63 – 653Combined sources
Beta strandi70 – 723Combined sources
Beta strandi74 – 763Combined sources
Helixi81 – 10020Combined sources
Helixi105 – 1073Combined sources
Beta strandi108 – 1136Combined sources
Helixi115 – 12511Combined sources
Beta strandi134 – 1396Combined sources
Helixi153 – 1564Combined sources
Beta strandi160 – 1656Combined sources
Beta strandi169 – 1713Combined sources
Helixi173 – 18412Combined sources
Turni185 – 1873Combined sources
Beta strandi190 – 1967Combined sources
Beta strandi198 – 2003Combined sources
Helixi203 – 21715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUOX-ray1.80A/B1-218[»]
1AURX-ray2.50A/B1-218[»]
ProteinModelPortaliQ53547.
SMRiQ53547. Positions 1-218.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53547.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106CJB. Bacteria.
COG0400. LUCA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEPLILQPA KPADACVIWL HGLGADRYDF MPVAEALQES LLTTRFVLPQ
60 70 80 90 100
APTRPVTING GYEMPSWYDI KAMSPARSIS LEELEVSAKM VTDLIEAQKR
110 120 130 140 150
TGIDASRIFL AGFSQGGAVV FHTAFINWQG PLGGVIALST YAPTFGDELE
160 170 180 190 200
LSASQQRIPA LCLHGQYDDV VQNAMGRSAF EHLKSRGVTV TWQEYPMGHE
210
VLPQEIHDIG AWLAARLG
Length:218
Mass (Da):23,880
Last modified:November 1, 1996 - v1
Checksum:i6F1D4557410E4CB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79600 Genomic DNA. Translation: AAC60403.1.
PIRiJU0277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79600 Genomic DNA. Translation: AAC60403.1.
PIRiJU0277.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUOX-ray1.80A/B1-218[»]
1AURX-ray2.50A/B1-218[»]
ProteinModelPortaliQ53547.
SMRiQ53547. Positions 1-218.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERipsefl-cxest. LYsophospholipase_carboxylesterase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4106CJB. Bacteria.
COG0400. LUCA.

Miscellaneous databases

EvolutionaryTraceiQ53547.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEST2_PSEFL
AccessioniPrimary (citable) accession number: Q53547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.