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Protein

30S ribosomal protein S12

Gene

rpsL

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

With S4 and S5 plays an important role in translational accuracy.By similarity
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S12
Gene namesi
Name:rpsL
Ordered Locus Names:Mb0701
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001462572 – 12430S ribosomal protein S12Add BLAST123

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ53538.
SMRiQ53538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S12P family.Curated

Phylogenomic databases

HOGENOMiHOG000040063.
KOiK02950.
OMAiTRYHIIR.

Family and domain databases

CDDicd03368. Ribosomal_S12. 1 hit.
HAMAPiMF_00403_B. Ribosomal_S12_B. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view]
PANTHERiPTHR11652. PTHR11652. 1 hit.
PfamiPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSiPR01034. RIBOSOMALS12.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTIQQLVRK GRRDKISKVK TAALKGSPQR RGVCTRVYTT TPKKPNSALR
60 70 80 90 100
KVARVKLTSQ VEVTAYIPGE GHNLQEHSMV LVRGGRVKDL PGVRYKIIRG
110 120
SLDTQGVKNR KQARSRYGAK KEKG
Length:124
Mass (Da):13,849
Last modified:January 23, 2007 - v4
Checksum:i2B441B040CEF20CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24L → P in AAB35201 (PubMed:7663416).Curated1
Sequence conflicti32G → R in AAB35201 (PubMed:7663416).Curated1
Sequence conflicti35T → N in AAB35201 (PubMed:7663416).Curated1
Sequence conflicti94R → Q in AAB35201 (PubMed:7663416).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79283 Genomic DNA. Translation: AAB35201.2.
BX248333 Genomic DNA. Translation: CDO41945.1.
PIRiS36888.
RefSeqiNP_854359.1. NC_002945.3.
WP_003403453.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO41945; CDO41945; Mb0701.
GeneIDi1091822.
KEGGimbo:Mb0701.
PATRICi18003200. VBIMycBov88188_0764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79283 Genomic DNA. Translation: AAB35201.2.
BX248333 Genomic DNA. Translation: CDO41945.1.
PIRiS36888.
RefSeqiNP_854359.1. NC_002945.3.
WP_003403453.1. NC_002945.3.

3D structure databases

ProteinModelPortaliQ53538.
SMRiQ53538.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO41945; CDO41945; Mb0701.
GeneIDi1091822.
KEGGimbo:Mb0701.
PATRICi18003200. VBIMycBov88188_0764.

Phylogenomic databases

HOGENOMiHOG000040063.
KOiK02950.
OMAiTRYHIIR.

Family and domain databases

CDDicd03368. Ribosomal_S12. 1 hit.
HAMAPiMF_00403_B. Ribosomal_S12_B. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view]
PANTHERiPTHR11652. PTHR11652. 1 hit.
PfamiPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSiPR01034. RIBOSOMALS12.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS12_MYCBO
AccessioniPrimary (citable) accession number: Q53538
Secondary accession number(s): Q9R547, X2BFV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 113 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Because the enzyme that would modify Asp-89 to 3-methylthioaspartic acid has not been found in the proteome of this organism, that modification is not predicted.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.