Reviewed, UniProtKB/Swiss-Prot Q53479 (IDSA_METTM)

Names and origin

Protein names

Recommended name:
    Bifunctional short chain isoprenyl diphosphate synthase
Including the following 2 domains:
    1- Recommended name:
            Farnesyl pyrophosphate synthetase
                Short name=FPP synthetase
              EC=2.5.1.1
        Alternative name(s):
            Dimethylallyltranstransferase
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10

Gene names

Name:

idsA

Organism

Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133)

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate.
Cofactor	Magnesium.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Transferase
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC geranyltranstransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 325	324	Bifunctional short chain isoprenyl diphosphate synthase		PRO_0000123972

Sequences

	Sequence		Length	Mass (Da)
	Q53479-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D28CAFF56EBE27E9	FASTA	325	35,636
	10 20 30 40 50 60 MTEVLDILRK YSEVADKRIM ECISDITPDT LLKASEHLIT AGGKKIRPSL ALLSCEAVGG 70 80 90 100 110 120 NPEDAAGVAA AIELIHTFSL IHDDIMDDDE MRRGEPSVHV IWGEPMAILA GDVLFSKAFE 130 140 150 160 170 180 AVIRNGDSER VKDALAVVVD SCVKICEGQA LDMGFEERLD VTEDEYMEMI YKKTAALIAA 190 200 210 220 230 240 ATKAGAIMGG ASEREVEALE DYGKFIGLAF QIHDDYLDVV SDEESLGKPV GSDIAEGKMT 250 260 270 280 290 300 LMVVKALEEA SEEDRERLIS ILGSGDEGSV AEAIEIFERY GATQYAHEVA LDYVRMAKER 310 320 LEILEDSDAR DALMRIADFV LEREH

References

[1]

"Isolation and characterization of idsA: the gene for the short chain isoprenyl diphosphate synthase from Methanobacterium thermoautotrophicum." Chen A., Poulter C.D. Arch. Biochem. Biophys. 314:399-404(1994) [PubMed: 7979381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S75695 Genomic DNA. Translation: AAB32421.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. [Graphical view]
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDSA_METTM

Accession

Primary (citable) accession number: Q53479

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 43 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)