Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

(S)-2-haloacid dehalogenase

Gene
N/A
Organism
Pseudomonas sp. (strain YL)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids. Acts on acids of short chain lengths, C2 to C4, with inversion of configuration at C-2.

Catalytic activityi

(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10Nucleophile1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-2-haloacid dehalogenase (EC:3.8.1.2)
Alternative name(s):
2-haloalkanoic acid dehalogenase
Halocarboxylic acid halidohydrolase
L-2-haloacid dehalogenase
L-DEX
OrganismiPseudomonas sp. (strain YL)
Taxonomic identifieri66693 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10D → A, E, G, N or S: Loss of activity. 1 Publication1
Mutagenesisi180D → E, G, N or S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000791661 – 232(S)-2-haloacid dehalogenaseAdd BLAST232

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Turni13 – 15Combined sources3
Helixi19 – 28Combined sources10
Turni30 – 32Combined sources3
Helixi33 – 54Combined sources22
Helixi60 – 75Combined sources16
Helixi81 – 89Combined sources9
Helixi90 – 93Combined sources4
Helixi100 – 109Combined sources10
Beta strandi113 – 120Combined sources8
Helixi122 – 131Combined sources10
Helixi135 – 137Combined sources3
Beta strandi139 – 144Combined sources6
Helixi145 – 147Combined sources3
Helixi154 – 164Combined sources11
Helixi168 – 170Combined sources3
Beta strandi171 – 176Combined sources6
Helixi178 – 187Combined sources10
Beta strandi191 – 194Combined sources4
Beta strandi203 – 205Combined sources3
Beta strandi209 – 214Combined sources6
Helixi215 – 219Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JUDX-ray2.50A1-232[»]
1QH9X-ray2.50A1-232[»]
1ZRMX-ray2.00A1-232[»]
1ZRNX-ray1.83A1-232[»]
ProteinModelPortaliQ53464.
SMRiQ53464.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53464.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR006328. HAD_II.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
PRINTSiPR00413. HADHALOGNASE.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01493. HAD-SF-IA-v2. 1 hit.
TIGR01428. HAD_type_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q53464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYIKGIAFD LYGTLFDVHS VVGRCDEAFP GRGREISALW RQKQLEYTWL
60 70 80 90 100
RSLMNRYVNF QQATEDALRF TCRHLGLDLD ARTRSTLCDA YLRLAPFSEV
110 120 130 140 150
PDSLRELKRR GLKLAILSNG SPQSIDAVVS HAGLRDGFDH LLSVDPVQVY
160 170 180 190 200
KPDNRVYELA EQALGLDRSA ILFVSSNAWD ATGARYFGFP TCWINRTGNV
210 220 230
FEEMGQTPDW EVTSLRAVVE LFETAAGKAE KG
Length:232
Mass (Da):26,177
Last modified:November 1, 1996 - v1
Checksum:iD276B5164B02E2C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S74078 Genomic DNA. Translation: AAB32245.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S74078 Genomic DNA. Translation: AAB32245.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JUDX-ray2.50A1-232[»]
1QH9X-ray2.50A1-232[»]
1ZRMX-ray2.00A1-232[»]
1ZRNX-ray1.83A1-232[»]
ProteinModelPortaliQ53464.
SMRiQ53464.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ53464.

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR006328. HAD_II.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
PRINTSiPR00413. HADHALOGNASE.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01493. HAD-SF-IA-v2. 1 hit.
TIGR01428. HAD_type_II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHAD_PSEUY
AccessioniPrimary (citable) accession number: Q53464
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.