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Protein

Xylanase/beta-glucanase

Gene

xynD

Organism
Ruminococcus flavefaciens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Contains two catalytic domains with xylanase and endo-beta-1,3-1,4 glucanase activities.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Pathwayi: xylan degradation

This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124NucleophileBy similarity1
Active sitei226Proton donorPROSITE-ProRule annotation1
Active sitei684NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiCBM22. Carbohydrate-Binding Module Family 22.
GH11. Glycoside Hydrolase Family 11.
GH16. Glycoside Hydrolase Family 16.
mycoCLAPiZXG11D_RUMFL.

Names & Taxonomyi

Protein namesi
Recommended name:
Xylanase/beta-glucanase
Including the following 2 domains:
Endo-1,4-beta-xylanase (EC:3.2.1.8)
Short name:
Xylanase
Endo-beta-1,3-1,4 glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Lichenase
Gene namesi
Name:xynD
OrganismiRuminococcus flavefaciens
Taxonomic identifieri1265 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000000800932 – 802Xylanase/beta-glucanaseAdd BLAST771

Proteomic databases

PRIDEiQ53317.

Structurei

3D structure databases

ProteinModelPortaliQ53317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 239GH11PROSITE-ProRule annotationAdd BLAST208
Domaini258 – 404CBM-cenCAdd BLAST147
Domaini434 – 513DockerinPROSITE-ProRule annotationAdd BLAST80
Domaini556 – 792GH16PROSITE-ProRule annotationAdd BLAST237

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni245 – 523BAdd BLAST279
Regioni524 – 555LinkerAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi524 – 529Poly-Thr6
Compositional biasi532 – 543Poly-ThrAdd BLAST12
Compositional biasi546 – 553Poly-Thr8

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 11 (cellulase G) family.Curated
In the C-terminal section; belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 dockerin domain.PROSITE-ProRule annotation
Contains 1 GH11 (glycosyl hydrolase family 11) domain.PROSITE-ProRule annotation
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.180. 1 hit.
2.60.120.200. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR003305. CenC_carb-bd.
IPR013320. ConA-like_dom.
IPR016134. Dockerin_dom.
IPR008979. Galactose-bd-like.
IPR013319. GH11/12.
IPR018208. GH11_AS_1.
IPR033119. GH11_AS_2.
IPR033123. GH11_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF02018. CBM_4_9. 1 hit.
PF00457. Glyco_hydro_11. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 2 hits.
SSF63446. SSF63446. 1 hit.
PROSITEiPS51766. DOCKERIN. 1 hit.
PS00776. GH11_1. 1 hit.
PS00777. GH11_2. 1 hit.
PS51761. GH11_3. 1 hit.
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q53317-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSIFKRYA AAVGLMASVL MFTAVPTTSN AADDQKTGKV GGFDWEMWNQ
60 70 80 90 100
NYTGTVSMNP GAGSFTCSWS GIENFLARMG KNYDDQKKNY KAFGDIVLTY
110 120 130 140 150
DVEYTPRGNS YMCIYGWTRN PLMEYYIVEG WGDWEPPGND GVDNFGTTTI
160 170 180 190 200
DGKTYKIRKS MRYNQPSIEG TKTFPQYWSV RTTSGSRNNT TNYMKDQVSV
210 220 230 240 250
TKHFDAWSKA GLDMSGTLYE VSLNIEGYRS NGSANVKSIS FDGGIDIPDP
260 270 280 290 300
EPIKPDENGY YLKENFESGE GNWSGRGSAK VKSSSGYDGT KGIFVSGRED
310 320 330 340 350
TWNGASINLD ELTFKAGETY SLGTAVMQDF ESSVDFKLTL QYTDADGKEN
360 370 380 390 400
YDEVKTVTAA KGQWVDLSNS SYTIPSGATG LVLYVEVPES KTDFYMDGAY
410 420 430 440 450
AGVKGTKPLI SISSQSVDPP VTEPTNPTNP TGPSVTKWGD ANCDGGVDLS
460 470 480 490 500
DAIFIMQFLA NPNKYGLTGT ETNHMTNQGK VNGDVCEHGS GLTEDDAVSI
510 520 530 540 550
QKYLIRAISE LPESYLEGHD PSKTTTTTTR ITTTTTTTTT TTTSKTTTTT
560 570 580 590 600
TTTSPAMHGG YRDLGTPMNT SATMISDFRT GKAGDFFASD GWTNGKPFDC
610 620 630 640 650
WWYKRNAVIN DGCLQLSIDQ KWTNDKNPDW DPRYSGGEFR TNNFYHYGYY
660 670 680 690 700
ECSMQAMKND GVVSSFFTYT GPSDDNPWDE IDIEILGKNT TQVQFNYYTN
710 720 730 740 750
GQGKHEKLYD LGFDSSEAYH TYGFDWQPNY IAWYVDGREV YRATQDIPKT
760 770 780 790 800
PGKIMMNAWP GLTVDDWLKA FNGRTPLTAH YQWVTYNKNG VQHSSQGQNP

WG
Length:802
Mass (Da):89,091
Last modified:November 1, 1997 - v2
Checksum:i2880A689647284AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S61204 Genomic DNA. Translation: AAB26620.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S61204 Genomic DNA. Translation: AAB26620.1.

3D structure databases

ProteinModelPortaliQ53317.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM22. Carbohydrate-Binding Module Family 22.
GH11. Glycoside Hydrolase Family 11.
GH16. Glycoside Hydrolase Family 16.
mycoCLAPiZXG11D_RUMFL.

Proteomic databases

PRIDEiQ53317.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00114.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.180. 1 hit.
2.60.120.200. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR003305. CenC_carb-bd.
IPR013320. ConA-like_dom.
IPR016134. Dockerin_dom.
IPR008979. Galactose-bd-like.
IPR013319. GH11/12.
IPR018208. GH11_AS_1.
IPR033119. GH11_AS_2.
IPR033123. GH11_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF02018. CBM_4_9. 1 hit.
PF00457. Glyco_hydro_11. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 2 hits.
SSF63446. SSF63446. 1 hit.
PROSITEiPS51766. DOCKERIN. 1 hit.
PS00776. GH11_1. 1 hit.
PS00777. GH11_2. 1 hit.
PS51761. GH11_3. 1 hit.
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXYND_RUMFL
AccessioniPrimary (citable) accession number: Q53317
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 5, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.