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Q53317

- XYND_RUMFL

UniProt

Q53317 - XYND_RUMFL

Protein

Xylanase/beta-glucanase

Gene

xynD

Organism
Ruminococcus flavefaciens
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Contains two catalytic domains with xylanase and endo-beta-1,3-1,4 glucanase activities.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
    Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei124 – 1241NucleophileBy similarity
    Active sitei226 – 2261Proton donorPROSITE-ProRule annotation
    Active sitei684 – 6841NucleophileBy similarity

    GO - Molecular functioni

    1. endo-1,4-beta-xylanase activity Source: UniProtKB-EC
    2. licheninase activity Source: UniProtKB-EC

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiCBM22. Carbohydrate-Binding Module Family 22.
    GH11. Glycoside Hydrolase Family 11.
    GH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xylanase/beta-glucanase
    Including the following 2 domains:
    Endo-1,4-beta-xylanase (EC:3.2.1.8)
    Short name:
    Xylanase
    Endo-beta-1,3-1,4 glucanase (EC:3.2.1.73)
    Alternative name(s):
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Lichenase
    Gene namesi
    Name:xynD
    OrganismiRuminococcus flavefaciens
    Taxonomic identifieri1265 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 802771Xylanase/beta-glucanasePRO_0000008009Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ53317.
    SMRiQ53317. Positions 575-790.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini258 – 404147CBM-cenCAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni32 – 244213A (xylanase)Add
    BLAST
    Regioni245 – 523279BAdd
    BLAST
    Regioni524 – 55532LinkerAdd
    BLAST
    Regioni556 – 802247C (beta-glucanase)Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi524 – 5296Poly-Thr
    Compositional biasi532 – 54312Poly-ThrAdd
    BLAST
    Compositional biasi546 – 5538Poly-Thr

    Sequence similaritiesi

    In the N-terminal section; belongs to the glycosyl hydrolase 11 (cellulase G) family.Curated
    In the C-terminal section; belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    2.60.120.180. 1 hit.
    2.60.120.200. 1 hit.
    2.60.120.260. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR003305. CenC_carb-bd.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR008979. Galactose-bd-like.
    IPR001137. Glyco_hydro_11.
    IPR013319. Glyco_hydro_11/12.
    IPR018208. Glyco_hydro_11_AS.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view]
    PfamiPF02018. CBM_4_9. 1 hit.
    PF00457. Glyco_hydro_11. 1 hit.
    PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF49899. SSF49899. 2 hits.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
    PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
    PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q53317-1 [UniParc]FASTAAdd to Basket

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    MKKSIFKRYA AAVGLMASVL MFTAVPTTSN AADDQKTGKV GGFDWEMWNQ    50
    NYTGTVSMNP GAGSFTCSWS GIENFLARMG KNYDDQKKNY KAFGDIVLTY 100
    DVEYTPRGNS YMCIYGWTRN PLMEYYIVEG WGDWEPPGND GVDNFGTTTI 150
    DGKTYKIRKS MRYNQPSIEG TKTFPQYWSV RTTSGSRNNT TNYMKDQVSV 200
    TKHFDAWSKA GLDMSGTLYE VSLNIEGYRS NGSANVKSIS FDGGIDIPDP 250
    EPIKPDENGY YLKENFESGE GNWSGRGSAK VKSSSGYDGT KGIFVSGRED 300
    TWNGASINLD ELTFKAGETY SLGTAVMQDF ESSVDFKLTL QYTDADGKEN 350
    YDEVKTVTAA KGQWVDLSNS SYTIPSGATG LVLYVEVPES KTDFYMDGAY 400
    AGVKGTKPLI SISSQSVDPP VTEPTNPTNP TGPSVTKWGD ANCDGGVDLS 450
    DAIFIMQFLA NPNKYGLTGT ETNHMTNQGK VNGDVCEHGS GLTEDDAVSI 500
    QKYLIRAISE LPESYLEGHD PSKTTTTTTR ITTTTTTTTT TTTSKTTTTT 550
    TTTSPAMHGG YRDLGTPMNT SATMISDFRT GKAGDFFASD GWTNGKPFDC 600
    WWYKRNAVIN DGCLQLSIDQ KWTNDKNPDW DPRYSGGEFR TNNFYHYGYY 650
    ECSMQAMKND GVVSSFFTYT GPSDDNPWDE IDIEILGKNT TQVQFNYYTN 700
    GQGKHEKLYD LGFDSSEAYH TYGFDWQPNY IAWYVDGREV YRATQDIPKT 750
    PGKIMMNAWP GLTVDDWLKA FNGRTPLTAH YQWVTYNKNG VQHSSQGQNP 800
    WG 802
    Length:802
    Mass (Da):89,091
    Last modified:November 1, 1997 - v2
    Checksum:i2880A689647284AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S61204 Genomic DNA. Translation: AAB26620.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S61204 Genomic DNA. Translation: AAB26620.1 .

    3D structure databases

    ProteinModelPortali Q53317.
    SMRi Q53317. Positions 575-790.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM22. Carbohydrate-Binding Module Family 22.
    GH11. Glycoside Hydrolase Family 11.
    GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    2.60.120.180. 1 hit.
    2.60.120.200. 1 hit.
    2.60.120.260. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR003305. CenC_carb-bd.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR008979. Galactose-bd-like.
    IPR001137. Glyco_hydro_11.
    IPR013319. Glyco_hydro_11/12.
    IPR018208. Glyco_hydro_11_AS.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view ]
    Pfami PF02018. CBM_4_9. 1 hit.
    PF00457. Glyco_hydro_11. 1 hit.
    PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF49899. SSF49899. 2 hits.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
    PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
    PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A bifunctional enzyme, with separate xylanase and beta(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens."
      Flint H.J., Martin J., McPherson C.A., Daniel A.S., Zhang J.-X.
      J. Bacteriol. 175:2943-2951(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 17.

    Entry informationi

    Entry nameiXYND_RUMFL
    AccessioniPrimary (citable) accession number: Q53317
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3