Reviewed,
UniProtKB/Swiss-Prot Q53317 (XYND_RUMFL)
Last modified
June 16, 2009.
Version 60.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Xylanase/beta-glucanase Including the following 2 domains: 1- Recommended name: Endo-1,4-beta-xylanase Short name=Xylanase EC=3.2.1.8 2- Recommended name: Endo-beta-1,3-1,4 glucanase EC=3.2.1.73 Alternative name(s): 1,3-1,4-beta-D-glucan 4-glucanohydrolase Lichenase | ||
| Gene names |
| ||
| Organism | Ruminococcus flavefaciens | ||
| Taxonomic identifier | 1265 [NCBI] | ||
| Taxonomic lineage | Bacteria › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › Ruminococcus |
Protein attributes
| Sequence length | 802 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Contains two catalytic domains with xylanase and endo-beta-1,3-1,4 glucanase activities. |
| Catalytic activity | Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans. Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds. |
| Pathway | |
| Sequence similarities | In the N-terminal section; belongs to the glycosyl hydrolase 11 (cellulase G) family. In the C-terminal section; belongs to the glycosyl hydrolase 16 family. Contains 1 CBM-cenC (cenC-type cellulose-binding) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Xylan degradation |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC licheninase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 31 | 31 | Potential | ||||||
| Chain | 32 – 802 | 771 | Xylanase/beta-glucanase | PRO_0000008009 | |||||
Regions | |||||||||
| Domain | 258 – 404 | 147 | CBM-cenC | ||||||
| Region | 32 – 244 | 213 | A (xylanase) | ||||||
| Region | 245 – 523 | 279 | B | ||||||
| Region | 524 – 555 | 32 | Linker | ||||||
| Region | 556 – 802 | 247 | C (beta-glucanase) | ||||||
| Compositional bias | 524 – 529 | 6 | Poly-Thr | ||||||
| Compositional bias | 532 – 543 | 12 | Poly-Thr | ||||||
| Compositional bias | 546 – 553 | 8 | Poly-Thr | ||||||
Sites | |||||||||
| Active site | 124 | 1 | Nucleophile By similarity | ||||||
| Active site | 226 | 1 | Proton donor By similarity | ||||||
| Active site | 684 | 1 | Nucleophile By similarity | ||||||
Sequences
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References
| [1] | "A bifunctional enzyme, with separate xylanase and beta(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens." Flint H.J., Martin J., McPherson C.A., Daniel A.S., Zhang J.-X. J. Bacteriol. 175:2943-2951(1993) [PubMed: 8491715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 17. |
Cross-references
Sequence databases | |
|---|---|
| S61204 Genomic DNA. Translation: AAB26620.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1AJK based on UniProtKB P23904. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | CBM22. Carbohydrate-Binding Module Family 22. GH11. Glycoside Hydrolase Family 11. GH16. Glycoside Hydrolase Family 16. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.73. 97801. 3.2.1.8. 97801. |
Family and domain databases | |
| InterPro | IPR008264. Beta_glucanase. IPR003305. CenC_carb_bd. IPR013320. ConA-like_subgrp. IPR001137. Glyco_hydro_11. IPR013319. Glyco_hydro_11/12_cat. IPR018208. Glyco_hydro_11_AS. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view] |
| Gene3D | G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit. G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit. |
| Pfam | PF02018. CBM_4_9. 1 hit. PF00457. Glyco_hydro_11. 1 hit. PF00722. Glyco_hydro_16. 1 hit. [Graphical view] |
| PRINTS | PR00737. GLHYDRLASE16. |
| PROSITE | PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit. PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit. PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | XYND_RUMFL | ||||||||
| Accession | Primary (citable) accession number: Q53317 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


