Nif-specific regulatory protein - Rhizobium sp. (strain NGR234)

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Q53206 (NIFA_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nif-specific regulatory protein

Gene names

Name:	nifA
Ordered Locus Names:	NGR_a01260
ORF Names:	y4uN

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium ›

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Required for activation of most nif operons, which are directly involved in nitrogen fixation.
Subunit structure	Interacts with sigma-54.
Sequence similarities	Contains 1 GAF domain. Contains 1 sigma-54 factor interaction domain.
Sequence caution	The sequence CAA92413.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nitrogen fixation Transcription Transcription regulation Two-component regulatory system
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding
Molecular function	Activator
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay signal transduction system Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 594

594

Nif-specific regulatory protein

PRO_0000081314

Regions

Domain

69 – 210

142

GAF

Domain

252 – 480

229

Sigma-54 factor interaction

Nucleotide binding

280 – 287

ATP Potential

Nucleotide binding

343 – 352

ATP Potential

DNA binding

566 – 585

H-T-H motif By similarity

Region

481 – 551

Inter-domain linker

Region

552 – 594

C-terminal DNA-binding domain

Sites

Metal binding

494

By similarity

Metal binding

499

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q53206 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 576E209DD9130381
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FASTA

594

64,886

        10         20         30         40         50         60 
MQAQALDNVS KAAFSRREPR AVRTTLKVFP RKNWLDECMT ELSPKTMHKR DLGCSGLYRI 

        70         80         90        100        110        120 
SKVLITSASL EIKLANVINT LPALLPMRRG AIVVVGAEGE PETTATFGVE PPSSGARHIA 

       130        140        150        160        170        180 
AKAAIDRIVA KGAPLVVPDT CKSELFQDEL QSIVSGTGQV TFIGVPMKAD QETLGTLWID 

       190        200        210        220        230        240 
RAKDGAATRT QFEEEVRFLS MVANLAARAV RLNGHESRDS RPIGEEGDRK ISGGDKELPE 

       250        260        270        280        290        300 
PTRQRPTRID WIVGESPALR QVVESVKVVA QTNSAVLLRG ESGTGKEFFA KAIHELSPRR 

       310        320        330        340        350        360 
KKPFVKLNCA ALSAGVLESE LFGHEKGAFT GAISQRAGRF ELADGGTLLL DEIGEISPAF 

       370        380        390        400        410        420 
QAKLLRVLQE GELERVGGTK TLKVDVRLIC ATNKDLETAV AEGEFRADLY YRINVVPLFL 

       430        440        450        460        470        480 
PPLRERNGDI PRLAKVFLDR FNRENNRNLE FTPAALEILS RCKFPGNVRE LENCVRRTAT 

       490        500        510        520        530        540 
LARSATIVPS DFSCQNDQCF SSMLGKTADR PLGSHSLNGL AMSKRLPVES PASLGYPAGP 

       550        560        570        580        590 
GGLTVAPHLS DRELLISAME KAGWVQAKAA RILGLTPRQV GYALRRHHIQ VKKI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable 'sequenase': a beginning." Freiberg C., Perret X., Broughton W.J., Rosenthal A. Genome Res. 6:590-600(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[3]	"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68203 Genomic DNA. Translation: CAA92413.1. Different initiation. U00090 Genomic DNA. Translation: AAB91886.1.
RefSeq	NP_444099.1. NC_000914.2.
3D structure databases
ProteinModelPortal	Q53206.
ModBase	Search...
Protein-protein interaction databases
STRING	394.NGR_a01260.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB91886; AAB91886; NGR_a01260.
GeneID	962486.
KEGG	rhi:NGR_a01260.
PATRIC	32300949. VBIRhiSp122450_0110.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3604.
HOGENOM	HOG000058487.
KO	K02584.
OMA	LSMKRAT.
Family and domain databases
Gene3D	1.10.10.60. 2 hits.
InterPro	IPR003593. AAA+_ATPase. IPR003018. GAF. IPR009057. Homeodomain-like. IPR002197. HTH_Fis. IPR010113. Nif-specific_regulatory_prot. IPR002078. Sigma_54_int. IPR025662. Sigma_54_int_dom_ATP-bd_1. IPR025943. Sigma_54_int_dom_ATP-bd_2. IPR025944. Sigma_54_int_dom_CS. [Graphical view]
Pfam	PF01590. GAF. 1 hit. PF02954. HTH_8. 1 hit. PF00158. Sigma54_activat. 1 hit. [Graphical view]
PRINTS	PR01590. HTHFIS.
SMART	SM00382. AAA. 1 hit. SM00065. GAF. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
TIGRFAMs	TIGR01817. nifA. 1 hit.
PROSITE	PS00675. SIGMA54_INTERACT_1. 1 hit. PS00676. SIGMA54_INTERACT_2. 1 hit. PS00688. SIGMA54_INTERACT_3. 1 hit. PS50045. SIGMA54_INTERACT_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIFA_RHISN

Accession

Primary (citable) accession number: Q53206

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Rhizobium plasmid pNGR234a

Rhizobium plasmid pNGR234a: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents