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Protein

FeMo cofactor biosynthesis protein NifB

Gene

nifB

Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co or M-cluster) found in the dinitrogenase enzyme of the nitrogenase complex in nitrogen-fixing microorganisms. NifB catalyzes the crucial step of radical SAM-dependent carbide insertion that occurs concomitant with the insertion of a 9th sulfur and the rearrangement/coupling of two [4Fe-4S] clusters into a [8Fe-9S-C] cluster, the precursor to the M-cluster.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per monomer. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. The two others probably act as substrate.By similarity

Pathwayi: Fe-Mo cofactor biosynthesis

This protein is involved in the pathway Fe-Mo cofactor biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway Fe-Mo cofactor biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi76Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi80Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Binding sitei82S-adenosyl-L-methionineBy similarity1
Metal bindingi83Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Binding sitei130S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei182S-adenosyl-L-methionineBy similarity1
Binding sitei234S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi307Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi310Iron-sulfur 2 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processNitrogen fixation
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00782

Names & Taxonomyi

Protein namesi
Recommended name:
FeMo cofactor biosynthesis protein NifB (EC:4.-.-.-)
Alternative name(s):
Nitrogenase cofactor maturase NifB
Radical SAM assemblase NifB
Gene namesi
Name:nifB
Ordered Locus Names:NGR_a01270
ORF Names:y4uM
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001530461 – 493FeMo cofactor biosynthesis protein NifBAdd BLAST493

Structurei

3D structure databases

ProteinModelPortaliQ53205
SMRiQ53205
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. NifB family.Curated

Phylogenomic databases

HOGENOMiHOG000225000
KOiK02585
OMAiNHPCYSE
OrthoDBiPOG091H0IV4

Family and domain databases

CDDicd00852 NifB, 1 hit
Gene3Di3.20.20.70, 1 hit
3.30.420.130, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR003731 Di-Nase_FeMo-co_biosynth
IPR036105 DiNase_FeMo-co_biosyn_sf
IPR000385 MoaA_NifB_PqqE_Fe-S-bd_CS
IPR005980 Nase_CF_NifB
IPR034165 NifB_C
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PANTHERiPTHR43787:SF7 PTHR43787:SF7, 1 hit
PfamiView protein in Pfam
PF02579 Nitro_FeMo-Co, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDG01068 FeMo_cofactor_biosynthesis_pro, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SUPFAMiSSF53146 SSF53146, 1 hit
TIGRFAMsiTIGR01290 nifB, 1 hit
PROSITEiView protein in PROSITE
PS01305 MOAA_NIFB_PQQE, 1 hit

Sequencei

Sequence statusi: Complete.

Q53205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPIISLES LTNTTSSGQL LTTAKSGGCA SSSCGSSSRP TDMDSATWEK
60 70 80 90 100
IKDHPCFSEE AHHYFARMHV AVAPACNIQC NYCNRKYDCA NESRPGVVSE
110 120 130 140 150
KLTPDQALRK VVAVANEVPQ LSVLGIAGPG DACYDWNKTR ATFERVASEI
160 170 180 190 200
PDIKLCISTN GLALPEHVDK LAEMNVSHVT ITINMVDPAV GEKIYPWIFY
210 220 230 240 250
GHRRYTGVDA AKILHEQQML GLEMLTARGI LTKINSVMIP GVNDLHLIEV
260 270 280 290 300
NKWVKERGAF LHNVMPLISD PAHGTSFGLT GQRGPNALEL KALHDRLEGG
310 320 330 340 350
AKLMRHCRQC RADAVGLLGT DRGQEFTLDH VPLEPHYDGA KRQAYREVVA
360 370 380 390 400
RIRDDHLEAK EKAIATVASA NIRGSLQVAV ATKGGGRINE HFGHAKEFQV
410 420 430 440 450
YEASQTGIKF VGHRKVEPYC HGGWGEDAAL AGIIAALDGI DIVLCARIGD
460 470 480 490
CPKERLMEAG IRATDTYGYD YIEAAISALH ATEFGTAPSQ ATA
Length:493
Mass (Da):53,507
Last modified:November 1, 1996 - v1
Checksum:i6C8F9A8F87E39AC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68203 Genomic DNA Translation: CAA92412.1
U00090 Genomic DNA Translation: AAB91885.1
RefSeqiNP_444098.1, NC_000914.2
WP_010875165.1, NC_000914.2

Genome annotation databases

EnsemblBacteriaiAAB91885; AAB91885; NGR_a01270
GeneIDi962467
KEGGirhi:NGR_a01270
PATRICifig|394.7.peg.111

Similar proteinsi

Entry informationi

Entry nameiNIFB_SINFN
AccessioniPrimary (citable) accession number: Q53205
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

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