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Q53205 (NIFB_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
FeMo cofactor biosynthesis protein NifB
Gene names
Name:nifB
Ordered Locus Names:NGR_a01270
ORF Names:y4uM
Encoded onPlasmid sym pNGR234a
OrganismRhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]
Taxonomic identifier394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Probably involved in the synthesis of the Fe-Mo cofactor.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sequence similarities

Belongs to the MoaA/NifB/PqqE family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493FeMo cofactor biosynthesis protein NifB
PRO_0000153046

Sites

Metal binding761Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding801Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding831Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q53205 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6C8F9A8F87E39AC8

FASTA49353,507
        10         20         30         40         50         60 
MSAPIISLES LTNTTSSGQL LTTAKSGGCA SSSCGSSSRP TDMDSATWEK IKDHPCFSEE 

        70         80         90        100        110        120 
AHHYFARMHV AVAPACNIQC NYCNRKYDCA NESRPGVVSE KLTPDQALRK VVAVANEVPQ 

       130        140        150        160        170        180 
LSVLGIAGPG DACYDWNKTR ATFERVASEI PDIKLCISTN GLALPEHVDK LAEMNVSHVT 

       190        200        210        220        230        240 
ITINMVDPAV GEKIYPWIFY GHRRYTGVDA AKILHEQQML GLEMLTARGI LTKINSVMIP 

       250        260        270        280        290        300 
GVNDLHLIEV NKWVKERGAF LHNVMPLISD PAHGTSFGLT GQRGPNALEL KALHDRLEGG 

       310        320        330        340        350        360 
AKLMRHCRQC RADAVGLLGT DRGQEFTLDH VPLEPHYDGA KRQAYREVVA RIRDDHLEAK 

       370        380        390        400        410        420 
EKAIATVASA NIRGSLQVAV ATKGGGRINE HFGHAKEFQV YEASQTGIKF VGHRKVEPYC 

       430        440        450        460        470        480 
HGGWGEDAAL AGIIAALDGI DIVLCARIGD CPKERLMEAG IRATDTYGYD YIEAAISALH 

       490 
ATEFGTAPSQ ATA 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable 'sequenase': a beginning."
Freiberg C., Perret X., Broughton W.J., Rosenthal A.
Genome Res. 6:590-600(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular basis of symbiosis between Rhizobium and legumes."
Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.
[3]"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems."
Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.
Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z68203 Genomic DNA. Translation: CAA92412.1.
U00090 Genomic DNA. Translation: AAB91885.1.
RefSeqNP_444098.1. NC_000914.2.

3D structure databases

ProteinModelPortalQ53205.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394.NGR_a01270.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91885; AAB91885; NGR_a01270.
GeneID962467.
KEGGrhi:NGR_a01270.
PATRIC32300951. VBIRhiSp122450_0111.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0535.
HOGENOMHOG000225000.
KOK02585.
OMAHHYFARM.
OrthoDBEOG64R611.

Enzyme and pathway databases

BioCycSFRE394:GBYN-6178-MONOMER.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
3.30.420.130. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR003731. Di-Nase_FeMo-co_biosynth.
IPR006638. Elp3/MiaB/NifB.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR005980. Nase_CF_NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF02579. Nitro_FeMo-Co. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
SUPFAMSSF53146. SSF53146. 1 hit.
TIGRFAMsTIGR01290. nifB. 1 hit.
PROSITEPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIFB_RHISN
AccessionPrimary (citable) accession number: Q53205
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rhizobium plasmid pNGR234a

Rhizobium plasmid pNGR234a: entries and gene names