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Q53205

- NIFB_RHISN

UniProt

Q53205 - NIFB_RHISN

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Protein

FeMo cofactor biosynthesis protein NifB

Gene

nifB

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Probably involved in the synthesis of the Fe-Mo cofactor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi80 – 801Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. catalytic activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. coenzyme biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSFRE394:GBYN-6178-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FeMo cofactor biosynthesis protein NifB
Gene namesi
Name:nifB
Ordered Locus Names:NGR_a01270
ORF Names:y4uM
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001054: Plasmid sym pNGR234a

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493FeMo cofactor biosynthesis protein NifBPRO_0000153046Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi394.NGR_a01270.

Structurei

3D structure databases

ProteinModelPortaliQ53205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MoaA/NifB/PqqE family.Curated

Phylogenomic databases

eggNOGiCOG0535.
HOGENOMiHOG000225000.
KOiK02585.
OMAiHHYFARM.
OrthoDBiEOG64R611.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.420.130. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR003731. Di-Nase_FeMo-co_biosynth.
IPR006638. Elp3/MiaB/NifB.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR005980. Nase_CF_NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF02579. Nitro_FeMo-Co. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
SUPFAMiSSF53146. SSF53146. 1 hit.
TIGRFAMsiTIGR01290. nifB. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q53205-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAPIISLES LTNTTSSGQL LTTAKSGGCA SSSCGSSSRP TDMDSATWEK
60 70 80 90 100
IKDHPCFSEE AHHYFARMHV AVAPACNIQC NYCNRKYDCA NESRPGVVSE
110 120 130 140 150
KLTPDQALRK VVAVANEVPQ LSVLGIAGPG DACYDWNKTR ATFERVASEI
160 170 180 190 200
PDIKLCISTN GLALPEHVDK LAEMNVSHVT ITINMVDPAV GEKIYPWIFY
210 220 230 240 250
GHRRYTGVDA AKILHEQQML GLEMLTARGI LTKINSVMIP GVNDLHLIEV
260 270 280 290 300
NKWVKERGAF LHNVMPLISD PAHGTSFGLT GQRGPNALEL KALHDRLEGG
310 320 330 340 350
AKLMRHCRQC RADAVGLLGT DRGQEFTLDH VPLEPHYDGA KRQAYREVVA
360 370 380 390 400
RIRDDHLEAK EKAIATVASA NIRGSLQVAV ATKGGGRINE HFGHAKEFQV
410 420 430 440 450
YEASQTGIKF VGHRKVEPYC HGGWGEDAAL AGIIAALDGI DIVLCARIGD
460 470 480 490
CPKERLMEAG IRATDTYGYD YIEAAISALH ATEFGTAPSQ ATA
Length:493
Mass (Da):53,507
Last modified:November 1, 1996 - v1
Checksum:i6C8F9A8F87E39AC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68203 Genomic DNA. Translation: CAA92412.1.
U00090 Genomic DNA. Translation: AAB91885.1.
RefSeqiNP_444098.1. NC_000914.2.

Genome annotation databases

EnsemblBacteriaiAAB91885; AAB91885; NGR_a01270.
GeneIDi962467.
KEGGirhi:NGR_a01270.
PATRICi32300951. VBIRhiSp122450_0111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68203 Genomic DNA. Translation: CAA92412.1 .
U00090 Genomic DNA. Translation: AAB91885.1 .
RefSeqi NP_444098.1. NC_000914.2.

3D structure databases

ProteinModelPortali Q53205.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 394.NGR_a01270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB91885 ; AAB91885 ; NGR_a01270 .
GeneIDi 962467.
KEGGi rhi:NGR_a01270.
PATRICi 32300951. VBIRhiSp122450_0111.

Phylogenomic databases

eggNOGi COG0535.
HOGENOMi HOG000225000.
KOi K02585.
OMAi HHYFARM.
OrthoDBi EOG64R611.

Enzyme and pathway databases

BioCyci SFRE394:GBYN-6178-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.30.420.130. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR003731. Di-Nase_FeMo-co_biosynth.
IPR006638. Elp3/MiaB/NifB.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR005980. Nase_CF_NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF02579. Nitro_FeMo-Co. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
SUPFAMi SSF53146. SSF53146. 1 hit.
TIGRFAMsi TIGR01290. nifB. 1 hit.
PROSITEi PS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable 'sequenase': a beginning."
    Freiberg C., Perret X., Broughton W.J., Rosenthal A.
    Genome Res. 6:590-600(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular basis of symbiosis between Rhizobium and legumes."
    Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
    Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiNIFB_RHISN
AccessioniPrimary (citable) accession number: Q53205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Rhizobium plasmid pNGR234a
    Rhizobium plasmid pNGR234a: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3