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Q53205

- NIFB_RHISN

UniProt

Q53205 - NIFB_RHISN

Protein

FeMo cofactor biosynthesis protein NifB

Gene

nifB

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Probably involved in the synthesis of the Fe-Mo cofactor.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi76 – 761Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi80 – 801Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. catalytic activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. coenzyme biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSFRE394:GBYN-6178-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FeMo cofactor biosynthesis protein NifB
    Gene namesi
    Name:nifB
    Ordered Locus Names:NGR_a01270
    ORF Names:y4uM
    Encoded oniPlasmid sym pNGR234a0 Publication
    OrganismiRhizobium sp. (strain NGR234)
    Taxonomic identifieri394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001054: Plasmid sym pNGR234a

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493FeMo cofactor biosynthesis protein NifBPRO_0000153046Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi394.NGR_a01270.

    Structurei

    3D structure databases

    ProteinModelPortaliQ53205.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MoaA/NifB/PqqE family.Curated

    Phylogenomic databases

    eggNOGiCOG0535.
    HOGENOMiHOG000225000.
    KOiK02585.
    OMAiHHYFARM.
    OrthoDBiEOG64R611.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.30.420.130. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR003731. Di-Nase_FeMo-co_biosynth.
    IPR006638. Elp3/MiaB/NifB.
    IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
    IPR005980. Nase_CF_NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF02579. Nitro_FeMo-Co. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53146. SSF53146. 1 hit.
    TIGRFAMsiTIGR01290. nifB. 1 hit.
    PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q53205-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAPIISLES LTNTTSSGQL LTTAKSGGCA SSSCGSSSRP TDMDSATWEK    50
    IKDHPCFSEE AHHYFARMHV AVAPACNIQC NYCNRKYDCA NESRPGVVSE 100
    KLTPDQALRK VVAVANEVPQ LSVLGIAGPG DACYDWNKTR ATFERVASEI 150
    PDIKLCISTN GLALPEHVDK LAEMNVSHVT ITINMVDPAV GEKIYPWIFY 200
    GHRRYTGVDA AKILHEQQML GLEMLTARGI LTKINSVMIP GVNDLHLIEV 250
    NKWVKERGAF LHNVMPLISD PAHGTSFGLT GQRGPNALEL KALHDRLEGG 300
    AKLMRHCRQC RADAVGLLGT DRGQEFTLDH VPLEPHYDGA KRQAYREVVA 350
    RIRDDHLEAK EKAIATVASA NIRGSLQVAV ATKGGGRINE HFGHAKEFQV 400
    YEASQTGIKF VGHRKVEPYC HGGWGEDAAL AGIIAALDGI DIVLCARIGD 450
    CPKERLMEAG IRATDTYGYD YIEAAISALH ATEFGTAPSQ ATA 493
    Length:493
    Mass (Da):53,507
    Last modified:November 1, 1996 - v1
    Checksum:i6C8F9A8F87E39AC8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68203 Genomic DNA. Translation: CAA92412.1.
    U00090 Genomic DNA. Translation: AAB91885.1.
    RefSeqiNP_444098.1. NC_000914.2.
    WP_010875165.1. NC_000914.2.

    Genome annotation databases

    EnsemblBacteriaiAAB91885; AAB91885; NGR_a01270.
    GeneIDi962467.
    KEGGirhi:NGR_a01270.
    PATRICi32300951. VBIRhiSp122450_0111.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68203 Genomic DNA. Translation: CAA92412.1 .
    U00090 Genomic DNA. Translation: AAB91885.1 .
    RefSeqi NP_444098.1. NC_000914.2.
    WP_010875165.1. NC_000914.2.

    3D structure databases

    ProteinModelPortali Q53205.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 394.NGR_a01270.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB91885 ; AAB91885 ; NGR_a01270 .
    GeneIDi 962467.
    KEGGi rhi:NGR_a01270.
    PATRICi 32300951. VBIRhiSp122450_0111.

    Phylogenomic databases

    eggNOGi COG0535.
    HOGENOMi HOG000225000.
    KOi K02585.
    OMAi HHYFARM.
    OrthoDBi EOG64R611.

    Enzyme and pathway databases

    BioCyci SFRE394:GBYN-6178-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.30.420.130. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR003731. Di-Nase_FeMo-co_biosynth.
    IPR006638. Elp3/MiaB/NifB.
    IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
    IPR005980. Nase_CF_NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF02579. Nitro_FeMo-Co. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53146. SSF53146. 1 hit.
    TIGRFAMsi TIGR01290. nifB. 1 hit.
    PROSITEi PS01305. MOAA_NIFB_PQQE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable 'sequenase': a beginning."
      Freiberg C., Perret X., Broughton W.J., Rosenthal A.
      Genome Res. 6:590-600(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular basis of symbiosis between Rhizobium and legumes."
      Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
      Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NGR234.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NGR234.

    Entry informationi

    Entry nameiNIFB_RHISN
    AccessioniPrimary (citable) accession number: Q53205
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Rhizobium plasmid pNGR234a
      Rhizobium plasmid pNGR234a: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3