Q53199 (DHE3_RHISN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamate dehydrogenase
Short name=GDH
EC=1.4.1.3

Gene names

Ordered Locus Names:	NGR_a01340
ORF Names:	y4uF

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Probable glutamate dehydrogenase		PRO_0000182753
Sites
	Active site	86	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q53199 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3D61C9122A9523BE	FASTA	443	48,845
	10 20 30 40 50 60 MSFLDLPEGL PERIIQCNSP YTVRFGVRLR GRMYSFIGWR SVREHCEPVK GDIRYASNAD 70 80 90 100 110 120 AEEVEALAAL MTLKCSLVDV PFGGSKGALK IDPRGWTPQE LEHITRRFTQ EMNKRPDRAR 130 140 150 160 170 180 RQCVGSDIGT GEREMAWMMD EFRRANPTDV VTSGACVTGK PLSKGGIAGR AESTGRGVQF 190 200 210 220 230 240 AIQSSLRDTR TPGLDGRRNL KGASTVIQGF GNVGYHAARF LSEEDDARVT VLAERDGYVA 250 260 270 280 290 300 NPEGLSIEAL KQHQIRTGSI LGFDGAKSIA GDMCGVEQPC DVLIPAAMEN AIHAENAERM 310 320 330 340 350 360 KAHLVVEAAN GPVTFEADEI LRSRGVTILP DLYVNAGGVV VSYFERVKNL THIPFGLMER 370 380 390 400 410 420 RRRERGNHTI ATALERMTGK ESPADMRDEF LEGGAEIDLV RSGLEDVMRS TWTRIADLME 430 440 QQPELGDYRT AAYVASIRQV ADL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable 'sequenase': a beginning." Freiberg C., Perret X., Broughton W.J., Rosenthal A. Genome Res. 6:590-600(1996) [PubMed: 8796346] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed: 9163424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
[3]	"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed: 19376903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NGR234.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68203 Genomic DNA. Translation: CAA92406.1. U00090 Genomic DNA. Translation: AAB91878.1.
RefSeq	NP_444091.1. NC_000914.2.
3D structure databases
ProteinModelPortal	Q53199.
ModBase	Search...
Protein-protein interaction databases
STRING	Q53199.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	962463.
GenomeReviews	Gene locus NGR_a01340 in contig U00090_GR.
KEGG	rhi:NGR_a01340.
PATRIC	32300963. VBIRhiSp122450_0117.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG590661.
OMA	MTYKCAL.
ProtClustDB	CLSK2461523.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00261.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_RHISN

Accession

Primary (citable) accession number: Q53199

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program