Reviewed, UniProtKB/Swiss-Prot Q53199 (DHE3_RHISN)

Names and origin

Protein names

Recommended name:
    Probable glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3

Gene names

Ordered Locus Names:	NGR_a01340
ORF Names:	y4uF

Encoded on

Plasmid sym pNGR234a

Organism

Rhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]

Taxonomic identifier

394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Probable glutamate dehydrogenase		PRO_0000182753
Sites
	Active site	86	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q53199-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3D61C9122A9523BE	FASTA	443	48,845
	10 20 30 40 50 60 MSFLDLPEGL PERIIQCNSP YTVRFGVRLR GRMYSFIGWR SVREHCEPVK GDIRYASNAD 70 80 90 100 110 120 AEEVEALAAL MTLKCSLVDV PFGGSKGALK IDPRGWTPQE LEHITRRFTQ EMNKRPDRAR 130 140 150 160 170 180 RQCVGSDIGT GEREMAWMMD EFRRANPTDV VTSGACVTGK PLSKGGIAGR AESTGRGVQF 190 200 210 220 230 240 AIQSSLRDTR TPGLDGRRNL KGASTVIQGF GNVGYHAARF LSEEDDARVT VLAERDGYVA 250 260 270 280 290 300 NPEGLSIEAL KQHQIRTGSI LGFDGAKSIA GDMCGVEQPC DVLIPAAMEN AIHAENAERM 310 320 330 340 350 360 KAHLVVEAAN GPVTFEADEI LRSRGVTILP DLYVNAGGVV VSYFERVKNL THIPFGLMER 370 380 390 400 410 420 RRRERGNHTI ATALERMTGK ESPADMRDEF LEGGAEIDLV RSGLEDVMRS TWTRIADLME 430 440 QQPELGDYRT AAYVASIRQV ADL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable 'sequenase': a beginning." Freiberg C., Perret X., Broughton W.J., Rosenthal A. Genome Res. 6:590-600(1996) [PubMed: 8796346] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular basis of symbiosis between Rhizobium and legumes." Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Nature 387:394-401(1997) [PubMed: 9163424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems." Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R. Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed: 19376903] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68203 Genomic DNA. Translation: CAA92406.1. U00090 Genomic DNA. Translation: AAB91878.1.
RefSeq	NP_444091.1.
3D structure databases
SMR	Q53199. Positions 4-442.
ModBase	Search...
Genome annotation databases
GeneID	962463.
GenomeReviews	Gene locus NGR_a01340 in contig U00090_GR.
KEGG	rhi:NGR_a01340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG590661.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_RHISN

Accession

Primary (citable) accession number: Q53199

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)