Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q53199 (DHE3_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamate dehydrogenase

Short name=GDH
EC=1.4.1.3
Gene names
Ordered Locus Names:NGR_a01340
ORF Names:y4uF
Encoded onPlasmid sym pNGR234a
OrganismRhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]
Taxonomic identifier394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Biological_processcellular amino acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutamate dehydrogenase [NAD(P)+] activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Probable glutamate dehydrogenase
PRO_0000182753

Sites

Active site861 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q53199 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3D61C9122A9523BE

FASTA44348,845
        10         20         30         40         50         60 
MSFLDLPEGL PERIIQCNSP YTVRFGVRLR GRMYSFIGWR SVREHCEPVK GDIRYASNAD 

        70         80         90        100        110        120 
AEEVEALAAL MTLKCSLVDV PFGGSKGALK IDPRGWTPQE LEHITRRFTQ EMNKRPDRAR 

       130        140        150        160        170        180 
RQCVGSDIGT GEREMAWMMD EFRRANPTDV VTSGACVTGK PLSKGGIAGR AESTGRGVQF 

       190        200        210        220        230        240 
AIQSSLRDTR TPGLDGRRNL KGASTVIQGF GNVGYHAARF LSEEDDARVT VLAERDGYVA 

       250        260        270        280        290        300 
NPEGLSIEAL KQHQIRTGSI LGFDGAKSIA GDMCGVEQPC DVLIPAAMEN AIHAENAERM 

       310        320        330        340        350        360 
KAHLVVEAAN GPVTFEADEI LRSRGVTILP DLYVNAGGVV VSYFERVKNL THIPFGLMER 

       370        380        390        400        410        420 
RRRERGNHTI ATALERMTGK ESPADMRDEF LEGGAEIDLV RSGLEDVMRS TWTRIADLME 

       430        440 
QQPELGDYRT AAYVASIRQV ADL 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234 using dye terminators and a thermostable 'sequenase': a beginning."
Freiberg C., Perret X., Broughton W.J., Rosenthal A.
Genome Res. 6:590-600(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular basis of symbiosis between Rhizobium and legumes."
Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.
[3]"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems."
Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.
Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z68203 Genomic DNA. Translation: CAA92406.1.
U00090 Genomic DNA. Translation: AAB91878.1.
RefSeqNP_444091.1. NC_000914.2.

3D structure databases

ProteinModelPortalQ53199.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394.NGR_a01340.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91878; AAB91878; NGR_a01340.
GeneID962463.
KEGGrhi:NGR_a01340.
PATRIC32300963. VBIRhiSp122450_0117.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0334.
HOGENOMHOG000243801.
KOK00261.
OMAELEHITR.
OrthoDBEOG65XN4D.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-5511-MONOMER.
RETL1328306-WGS:GSTH-6401-MONOMER.
RETL1328306-WGS:GSTH-6459-MONOMER.
SFRE394:GBYN-6185-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE3_RHISN
AccessionPrimary (citable) accession number: Q53199
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rhizobium plasmid pNGR234a

Rhizobium plasmid pNGR234a: entries and gene names