Reviewed, UniProtKB/Swiss-Prot Q53139 (COBK_RHOER)

Names and origin

Protein names

Recommended name:
    Precorrin-6A reductase
    EC=1.3.1.54
Alternative name(s):
    Precorrin-6X reductase

Gene names

Name:

cobK

Organism

Rhodococcus erythropolis

Taxonomic identifier

1833 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the macrocycle of precorrin-6X into precorrin-6Y.
Catalytic activity	Precorrin-6B + NADP+ = precorrin-6A + NADPH.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinic acid a,c-diamide from precorrin-2 (aerobic route): step 6/10.
Sequence similarities	Belongs to the precorrin-6x reductase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	precorrin-6A reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Precorrin-6A reductase		PRO_0000135922

Sequences

	Sequence		Length	Mass (Da)
	Q53139-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2BA2B2D6810C4623	FASTA	248	26,142
	10 20 30 40 50 60 MTRILILGGT GEARALAAAL ADVPGVEAVS SLAGRVRDPR LPVGDVRIGG FGGADGLAEC 70 80 90 100 110 120 VRAHPVDAIV DATHPFAAQI TRNAADAAHR RGIPLVVLRR PEWSPRPGEH WHGAADLADA 130 140 150 160 170 180 AELLPDLGTR IFLTIGRQGV DAFADLQALW FLIRAIDPPD VAMPPHSTLL LARGPFAVAD 190 200 210 220 230 240 ETALMREHRI DVLVTKNSGG GQTDAKLDAA RALGIPVLMI RRPPLPPATE TVDDVAGAIA WVGTLTRR

References

[1]	"Sequences of the cobalamin biosynthetic genes cobK, cobL and cobM from Rhodococcus sp. NI86/21." de Mot R., Nagy I., Schoofs G., Vanderleyden J. Gene 143:91-93(1994) [PubMed: 8200543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NI86/21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L21196 Genomic DNA. Translation: AAC37131.1.
PIR	T46837.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.54. 1430.
Family and domain databases
InterPro	IPR003723. Cbl_synth_CobK/CbiJ. [Graphical view]
Pfam	PF02571. CbiJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00715. precor6x_red. 1 hit.
PROSITE	PS51014. COBK_CBIJ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COBK_RHOER

Accession

Primary (citable) accession number: Q53139

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)