Q53135 (CHEA_RHOSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis protein CheA
EC=2.7.13.3

Gene names

Name:

cheA

Organism

Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)

Taxonomic identifier

1063 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either CheB or CheY By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Contains 1 cheW-like domain. Contains 1 histidine kinase domain. Contains 1 HPt domain.

Ontologies

Keywords
Biological process	Chemotaxis Two-component regulatory system
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Chemotaxis protein CheA		PRO_0000074717
Regions
	Domain	1 – 106	106	HPt
	Domain	290 – 540	251	Histidine kinase
	Domain	542 – 678	137	CheW-like
Amino acid modifications
	Modified residue	49	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q53135 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 167B6B8BDF756E03	FASTA	686	74,291
	10 20 30 40 50 60 MDQSDIRSAF FVECEDLMEA LNEGLDRIED TLDDGHDDET VNAVFRAVHS IKGGAGAFKL 70 80 90 100 110 120 DALVRFAHQF ETTLDALRAG RVSADPPLLA LLHKAADRLS DLLQAARTGS ETATIDPDDL 130 140 150 160 170 180 VAQLAQAAGE EEAGEADAED LGFVPMRLDL DLPAAAPDEG QSGCYSIDFS PTRALYACGN 190 200 210 220 230 240 DTSILFRVLS DLGRMEVRLD RSRLPDFDAL DWQESWLDWH LTLETDEPEH QIDEVFEFVE 250 260 270 280 290 300 DLCRLEIRPM AMEAPRTDPD PEPDPEPDPP ASGPAAAKKS PAEDHRTSSP RATVRVELDR 310 320 330 340 350 360 VDRLINIVGE LVINQAMLSQ CVQDEGVPPR SPVRNRLDDF RNLAREIQES VMAIRAQAIK 370 380 390 400 410 420 PLFQRMSRIA REASEISQKQ IRLVTEGEST EVDKTVIERL ADPLTHMIRN AVDHGIEPAD 430 440 450 460 470 480 RRLHLGKPPV GLITLTAAHR SGRVLIEIKD DGAGINRPRV LEIAQGKGLV AQDAQLTEEE 490 500 510 520 530 540 IDGLLFMPGF STASVVSDLS GRGVGMDVVK SAIESLGGRI TIASDPGVGT TFTISLPLTL 550 560 570 580 590 600 AVLDGMVVDV GGQTMVVPVS AIVETLRPRP ADIHVLGSGD QVVAIRGSLV PIVDCGSIFG 610 620 630 640 650 660 FRAPVRSYEE SVLLLVETAR QKLCALVVDT IHDQRQVVIK GLENGYGRIP GVAAATILGD 670 680 GRIALIIAPE EAVDIGTSGG TFSMEF

References

[1]	"Identification of a chemotaxis operon with two cheY genes in Rhodobacter sphaeroides." Ward M.J., Bell A.W., Hamblin P.A., Packer H.L., Armitage J.P. Mol. Microbiol. 17:357-366(1995) [PubMed: 7494484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WS8N.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80027 Genomic DNA. Translation: CAA56330.1.
PIR	S70180.
3D structure databases
ProteinModelPortal	Q53135.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.13.3. 5383.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR002545. CheW. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_Hpt_dom. IPR004105. Sig_transdc_His_kin_subgr_dim. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:1.20.120.160. Sig_transdc_His_kin_Hpt_dom. 1 hit. G3DSA:1.10.287.560. Sig_transdc_His_kin_subgr_dim. 1 hit.
Pfam	PF01584. CheW. 1 hit. PF02895. H-kinase_dim. 1 hit. PF02518. HATPase_c. 1 hit. PF01627. Hpt. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00260. CheW. 1 hit. SM00387. HATPase_c. 1 hit. SM00073. HPT. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF50341. CheW. 1 hit. SSF47384. His_kin_homodim. 1 hit. SSF47226. Hpt. 1 hit.
PROSITE	PS50851. CHEW. 1 hit. PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEA_RHOSH

Accession

Primary (citable) accession number: Q53135

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program