Q53020 (OMPB_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer membrane protein B

Alternative name(s):
168 kDa surface-layer protein
Cell surface antigen 5
Short name=Sca5
Surface protein antigen
rOmp B
Short name=rOmpB

Cleaved into the following 2 chains:

1. 120 kDa surface-exposed protein
   Alternative name(s):
   120 kDa outer membrane protein OmpB
   Surface protein antigen
   p120
2. 32 kDa beta peptide

Gene names

Name:	ompB
Synonyms:	spa, spaP
Ordered Locus Names:	RP704

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	1643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The 120 kDa surface-exposed protein is a major structural protein which may play a role as a rickettsial virulence factor and/or immunogen during infection. The 32 kDa beta peptide may serve as a membrane anchor. It has been shown to adhere to biotinylated Vero cell proteins.
Subcellular location	Outer membrane protein B: Periplasm By similarity. 120 kDa surface-exposed protein: Secreted. Cell surface. Note: Surface exposed. This bacterium is covered by a S-layer with hexagonal symmetry. 32 kDa beta peptide: Cell outer membrane; Multi-pass membrane protein Potential. Note: The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane.
Sequence similarities	Belongs to the rickettsiae OmpA/OmpB family. Contains 1 autotransporter (TC 1.B.12) domain. [View classification]
Sequence caution	The sequence AAA26390.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cell membrane Cell outer membrane Membrane Periplasm Secreted
Domain	Transmembrane Transmembrane beta strand
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1643	1643	Outer membrane protein B		PRO_0000387581
	Chain	1 – 1328	1328	120 kDa surface-exposed protein		PRO_0000032654
	Propeptide	1329 – 1352	24	Potential		PRO_0000032655
	Chain	1353 – 1643	291	32 kDa beta peptide		PRO_0000032656
Regions
	Domain	1355 – 1643	289	Autotransporter
Natural variations
	Natural variant	257	1	V → A in strain: Breinl.
	Natural variant	1010	1	Y → D in strain: Breinl.
	Natural variant	1450	1	A → S in strain: Breinl.
Experimental info
	Sequence conflict	178 – 179	2	AA → VC in AAA26390. Ref.1
	Sequence conflict	191 – 201	11	TTQEAPLTLGA → INSRSSSYHLVS in AAA26390. Ref.1
	Sequence conflict	212	1	T → I in AAA26390. Ref.1
	Sequence conflict	313	1	Q → L in AAA26390. Ref.1
	Sequence conflict	1104	1	D → G in AAD42234. Ref.2
	Sequence conflict	1123	1	T → S in AAD42234. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q53020 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 735FDF392E6346CC	FASTA	1,643	169,855
	10 20 30 40 50 60 MAQKPNFLKK IISAGLVTAS TATIVAGFSG VAMGAAMQYN RTTNAAATTF DGIGFDQAAG 70 80 90 100 110 120 ANIPVAPNSV ITANANNPIT FNTPNGHLNS LFLDTANDLA VTINEDTTLG FITNIAQQAK 130 140 150 160 170 180 FFNFTVAAGK ILNITGQGIT VQEASNTINA QNALTKVHGG AAINANDLSG LGSITFAAAP 190 200 210 220 230 240 SVLEFNLINP TTQEAPLTLG ANSKIVNGGN GTLNITNGFI QVSDNTFAGI KTINIDDCQG 250 260 270 280 290 300 LMFNSTPDAA NTLNLQVGGN TINFNGIDGT GKLVLVSKNG AATEFNVTGT LGGNLKGIIE 310 320 330 340 350 360 LNTAAVAGKL ISQGGAANAV IGTDNGAGRA AGFIVSVDNG NAATISGQVY AKNMVIQSAN 370 380 390 400 410 420 AGGQVTFEHI VDVGLGGTTN FKTADSKVII TENSNFGSTN FGNLDTQIVV PDTKILKGNF 430 440 450 460 470 480 IGDVKNNGNT AGVITFNANG ALVSASTDPN IAVTNINAIE AEGAGVVELS GIHIAELRLG 490 500 510 520 530 540 NGGSIFKLAD GTVINGPVNQ NALMNNNALA AGSIQLDGSA IITGDIGNGG VNAALQHITL 550 560 570 580 590 600 ANDASKILAL DGANIIGANV GGAIHFQANG GTIKLTNTQN NIVVNFDLDI TTDKTGVVDA 610 620 630 640 650 660 SSLTNNQTLT INGSIGTVVA NTKTLAQLNI GSSKTILNAG DVAINELVIE NNGSVQLNHN 670 680 690 700 710 720 TYLITKTINA ANQGQIIVAA DPLNTNTTLA DGTNLGSAEN PLSTIHFATK AANADSILNV 730 740 750 760 770 780 GKGVNLYANN ITTNDANVGS LHFRSGGTSI VSGTVGGQQG HKLNNLILDN GTTVKFLGDT 790 800 810 820 830 840 TFNGGTKIEG KSILQISNNY TTDHVESADN TGTLEFVNTD PITVTLNKQG AYFGVLKQVI 850 860 870 880 890 900 ISGPGNIVFN EIGNVGIVHG IAANSISFEN ASLGTSLFLP SGTPLDVLTI KSTVGNGTVD 910 920 930 940 950 960 NFNAPIVVVS GIDSMINNGQ IIGDKKNIIA LSLGSDNSIT VNANTLYSGI RTTKNNQGTV 970 980 990 1000 1010 1020 TLSGGMPNNP GTIYGLGLEN GSPKLKQVTF TTDYNNLGSI IANNVTINDY VTLTTGGIAG 1030 1040 1050 1060 1070 1080 TDFDAKITLG SVNGNANVRF VDSTFSDPRS MIVATQANKG TVTYLGNALV SNIGSLDTPV 1090 1100 1110 1120 1130 1140 ASVRFTGNDS GAGLQGNIYS QNIDFGTYNL TILNSNVILG GGTTAINGEI DLLTNNLIFA 1150 1160 1170 1180 1190 1200 NGTSTWGDNT SISTTLNVSS GNIGQVVIAE DAQVNATTTG TTTIKIQDNA NANFSGTQAY 1210 1220 1230 1240 1250 1260 TLIQGGARFN GTLGAPNFAV TGSNIFVKYE LIRDSNQDYV LTRTNDVLNV VTTAVGNSAI 1270 1280 1290 1300 1310 1320 ANAPGVSQNI SRCLESTNTA AYNNMLLAKD PSDVATFVGA IATDTSAAVT TVNLNDTQKT 1330 1340 1350 1360 1370 1380 QDLLSNRLGT LRYLSNAETS DVAGSATGAV SSGDEAEVSY GVWAKPFYNI AEQDKKGGIA 1390 1400 1410 1420 1430 1440 GYKAKTTGVV VGLDTLASDN LMIGAAIGIT KTDIKHQDYK KGDKTDINGL SFSLYGSQQL 1450 1460 1470 1480 1490 1500 VKNFFAQGNA IFTLNKVKSK SQRYFFESNG KMSKQIAAGN YDNMTFGGNL IFGYDYNAMP 1510 1520 1530 1540 1550 1560 NVLVTPMAGL SYLKSSNENY KETGTTVANK RINSKFSDRV DLIVGAKVAG STVNITDIVI 1570 1580 1590 1600 1610 1620 YPEIHSFVVH KVNGKLSNSQ SMLDGQTAPF ISQPDRTAKT SYNIGLSANI KSDAKMEYGI 1630 1640 GYDFNSASKY TAHQGTLKVR VNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the gene encoding the protective paracrystalline-surface-layer protein of Rickettsia prowazekii: presence of a truncated identical homolog in Rickettsia typhi." Carl M., Dobson M.E., Ching W.M., Dasch G.A. Proc. Natl. Acad. Sci. U.S.A. 87:8237-8241(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC VR-142 / Breinl.
[2]	"Sequence analysis of ompB of Rickettsia prowazekii." Moron C.G., Yu X.J., Walker D.H. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC VR-142 / Breinl.
[3]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[4]	"Mapping of monoclonal antibody binding sites on CNBr fragments of the S-layer protein antigens of Rickettsia typhi and Rickettsia prowazekii." Ching W.M., Carl M., Dasch G.A. Mol. Immunol. 29:95-105(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: ATCC VR-142 / Breinl.
[5]	"Evidence for proteolytic cleavage of the 120-kilodalton outer membrane protein of rickettsiae: identification of an avirulent mutant deficient in processing." Hackstadt T., Messer R., Cieplak W. Jr., Peacock M.G. Infect. Immun. 60:159-165(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1353-1369, CLEAVAGE SITE. Strain: ATCC VR-142 / Breinl.
[6]	"Identification of two putative rickettsial adhesins by proteomic analysis." Renesto P., Samson L., Ogata H., Azza S., Fourquet P., Gorvel J.-P., Heinzen R.A., Raoult D. Res. Microbiol. 157:605-612(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ADHESION TO BIOTINYLATED EUKARYOTIC CELLS. Strain: ATCC VR-142 / Breinl.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37647 Genomic DNA. Translation: AAA26390.1. Different initiation. AF161079 Genomic DNA. Translation: AAD42234.1. AJ235273 Genomic DNA. Translation: CAA15140.1.
PIR	D71630.
RefSeq	NP_221064.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q53020.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA15140; CAA15140; CAA15140.
GeneID	883694.
KEGG	rpr:RP704.
PATRIC	17902241. VBIRicPro72556_0725.
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000010784.
OMA	TINAANQ.
ProtClustDB	CLSK871043.
Family and domain databases
InterPro	IPR005546. Auto_transptbeta. IPR006315. OM_autotransptr_brl. IPR022095. rOmpB. [Graphical view]
Pfam	PF03797. Autotransporter. 1 hit. PF12334. rOmpB. 1 hit. [Graphical view]
SMART	SM00869. Autotransporter. 1 hit. [Graphical view]
SUPFAM	SSF103515. Auto_transptbeta. 1 hit.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OMPB_RICPR

Accession

Primary (citable) accession number: Q53020
Secondary accession number(s): Q9ZCM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program