Reviewed, UniProtKB/Swiss-Prot Q52RG7 (SGPL_ORYSJ)

Names and origin

Protein names

Recommended name:
    Sphingosine-1-phosphate lyase
      Short name=SP-lyase
      Short name=SPL
    EC=4.1.2.27
Alternative name(s):
    Sphingosine-1-phosphate aldolase

Gene names

Name:	SPL
Ordered Locus Names:	Os01g0100900, LOC_Os01g01080
ORF Names:	P0402A09.4, P0672D08.8

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis By similarity.
Catalytic activity	Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.
Sequence similarities	Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.
Sequence caution	The sequence BAB62623.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD45497.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Apoptosis Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Signal-anchor Transmembrane
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW carboxylic acid metabolic process Inferred from electronic annotation. Source: InterPro lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: InterPro pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro sphinganine-1-phosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 46	46	Potential
	Chain	47 – 539	493	Sphingosine-1-phosphate lyase		PRO_0000247464
Regions
	Topological domain	47 – 54	8	Lumenal Potential
	Transmembrane	55 – 75	21	Potential
	Topological domain	76 – 539	464	Cytoplasmic Potential
Amino acid modifications
	Modified residue	344	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	365 – 369	5	PYFFS → HQFVA in AAX89367. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q52RG7-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: A690FABE1C24331D	FASTA	539	58,331
	10 20 30 40 50 60 MELAMDFALR LRDAANHHLS RYEPLVLLAA PLLALLAART LHAAAAAVAD RGLRTVLLAL 70 80 90 100 110 120 AMTAIKLLPG VSAYINAEKR KVVDQLQSGG TSTKSTLRTE LPTVGLSNQV INDLETLKAR 130 140 150 160 170 180 DVNWQGKCSG TVYIAGSESE GHFALINKAY SMFSHTNPLH QDVFKSVAQL EAEVVAMTAA 190 200 210 220 230 240 LLGIKEKSSG GQICGNMTSG GTESILLAVK TSRDYMRTKK GITKPEMIIA ESAHSAYDKA 250 260 270 280 290 300 AQYFNIKVRR VPVNKEFLAD VKGFKRCING NTIMMVGSAP GFPHGLIDPI EELGELASRY 310 320 330 340 350 360 DICLHVDLCL GGFVLPFARK LGYPIPPFDF CVKGVTSIST DVHKYGLAPK GTSIVLYKNH 370 380 390 400 410 420 EIRKPYFFSV TEWTGGLYVS PTIAGSRPGG LIAGAWAAMT SLGLNGYMEN TGHIMEVSKK 430 440 450 460 470 480 IQRGIEDIPG LFVIGKPDMT VVAFGSDSVD IFEVNDIMSS KGWHLNALQR PNSLHICVTL 490 500 510 520 530 QHTVIYEEFL KDLKDSVDTV KANPGPISGG RAPIYGAAGK MPDRGMVREL LVEFMDASC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and functional analysis of putative sphingosine-1-phosphate lyases from Arabidopsis thaliana and rice." Niu Y., Wang J., Liu K., Wang D. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY972084 mRNA. Translation: AAX89367.1. AP003610 Genomic DNA. Translation: BAB62623.1. Sequence problems. AP003727 Genomic DNA. Translation: BAD45497.1. Sequence problems.
3D structure databases
SMR	Q52RG7. Positions 94-498, 145-504.
ModBase	Search...
Organism-specific databases
Gramene	Q52RG7.
Family and domain databases
InterPro	IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SGPL_ORYSJ

Accession

Primary (citable) accession number: Q52RG7
Secondary accession number(s): Q655L2, Q7F2G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	January 19, 2010
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)