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Protein

Rho GTPase-activating protein 29

Gene

ARHGAP29

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.2 Publications

Caution

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri612 – 657Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST46

GO - Molecular functioni

  • GTPase activator activity Source: Reactome
  • metal ion binding Source: UniProtKB-KW
  • PDZ domain binding Source: MGI

GO - Biological processi

  • regulation of small GTPase mediated signal transduction Source: Reactome
  • Rho protein signal transduction Source: ProtInc

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 29
Alternative name(s):
PTPL1-associated RhoGAP protein 1
Rho-type GTPase-activating protein 29
Gene namesi
Name:ARHGAP29
Synonyms:PARG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000137962.12
HGNCiHGNC:30207 ARHGAP29
MIMi610496 gene
neXtProtiNX_Q52LW3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi9411
OpenTargetsiENSG00000137962
PharmGKBiPA128394548

Polymorphism and mutation databases

BioMutaiARHGAP29
DMDMi166977701

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003175821 – 1261Rho GTPase-activating protein 29Add BLAST1261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphoserineBy similarity1
Modified residuei176PhosphoserineCombined sources1
Modified residuei179PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Modified residuei499PhosphoserineCombined sources1
Modified residuei519PhosphoserineBy similarity1
Modified residuei552PhosphoserineBy similarity1
Modified residuei913PhosphoserineBy similarity1
Modified residuei949PhosphoserineCombined sources1
Modified residuei1019PhosphoserineCombined sources1
Modified residuei1144PhosphoserineBy similarity1
Modified residuei1146PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ52LW3
MaxQBiQ52LW3
PaxDbiQ52LW3
PeptideAtlasiQ52LW3
PRIDEiQ52LW3

PTM databases

iPTMnetiQ52LW3
PhosphoSitePlusiQ52LW3

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in skeletal muscle and heart. Expressed at intermediate level in placenta, liver and pancreas. Weakly expressed in brain, lung and kidney.1 Publication

Inductioni

Strongly down-regulated in mantle-cell lymphomas. Up-regulated in migrating glioma cells.2 Publications

Gene expression databases

BgeeiENSG00000137962
CleanExiHS_ARHGAP29
ExpressionAtlasiQ52LW3 baseline and differential
GenevisibleiQ52LW3 HS

Organism-specific databases

HPAiHPA026534

Interactioni

Subunit structurei

Interacts with PTPN13/PTPL1. Interacts with RAP2A via its coiled coil domain. Interacts with RASIP1 (By similarity).By similarity

GO - Molecular functioni

  • PDZ domain binding Source: MGI

Protein-protein interaction databases

BioGridi11480611 interactors.
IntActiQ52LW3 9 interactors.
MINTiQ52LW3
STRINGi9606.ENSP00000260526

Structurei

3D structure databases

ProteinModelPortaliQ52LW3
SMRiQ52LW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 462F-BARPROSITE-ProRule annotationAdd BLAST271
Domaini671 – 886Rho-GAPPROSITE-ProRule annotationAdd BLAST216

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1258 – 1261Interaction with PTPN13/PTPL11 Publication4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili296 – 418Sequence analysisAdd BLAST123

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri612 – 657Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST46

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1453 Eukaryota
ENOG410YM3I LUCA
GeneTreeiENSGT00910000144080
HOGENOMiHOG000043077
HOVERGENiHBG108406
InParanoidiQ52LW3
KOiK20644
OMAiDPGQEYS
OrthoDBiEOG091G00SA
PhylomeDBiQ52LW3
TreeFamiTF351450

Family and domain databases

CDDicd00029 C1, 1 hit
Gene3Di1.10.555.101 hit
1.20.1270.601 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR002219 PE/DAG-bd
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF48350 SSF48350, 2 hits
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50238 RHOGAP, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q52LW3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIAHKQKKTK KKRAWASGQL STDITTSEMG LKSLSSNSIF DPDYIKELVN
60 70 80 90 100
DIRKFSHMLL YLKEAIFSDC FKEVIHIRLE ELLRVLKSIM NKHQNLNSVD
110 120 130 140 150
LQNAAEMLTA KVKAVNFTEV NEENKNDLFQ EVFSSIETLA FTFGNILTNF
160 170 180 190 200
LMGDVGNDSL LRLPVSRETK SFENVSVESV DSSSEKGNFS PLELDNVLLK
210 220 230 240 250
NTDSIELALS YAKTWSKYTK NIVSWVEKKL NLELESTRNM VKLAEATRTN
260 270 280 290 300
IGIQEFMPLQ SLFTNALLND IESSHLLQQT IAALQANKFV QPLLGRKNEM
310 320 330 340 350
EKQRKEIKEL WKQEQNKMLE AENALKKAKL LCMQRQDEYE KAKSSMFRAE
360 370 380 390 400
EEHLSSSGGL AKNLNKQLEK KRRLEEEALQ KVEEANELYK VCVTNVEERR
410 420 430 440 450
NDLENTKREI LAQLRTLVFQ CDLTLKAVTV NLFHMQHLQA ASLADSLQSL
460 470 480 490 500
CDSAKLYDPG QEYSEFVKAT NSTEEEKVDG NVNKHLNSSQ PSGFGPANSL
510 520 530 540 550
EDVVRLPDSS NKIEEDRCSN SADITGPSFI RSWTFGMFSD SESTGGSSES
560 570 580 590 600
RSLDSESISP GDFHRKLPRT PSSGTMSSAD DLDEREPPSP SETGPNSLGT
610 620 630 640 650
FKKTLMSKAA LTHKFRKLRS PTKCRDCEGI VVFQGVECEE CLLVCHRKCL
660 670 680 690 700
ENLVIICGHQ KLPGKIHLFG AEFTQVAKKE PDGIPFILKI CASEIENRAL
710 720 730 740 750
CLQGIYRVCG NKIKTEKLCQ ALENGMHLVD ISEFSSHDIC DVLKLYLRQL
760 770 780 790 800
PEPFILFRLY KEFIDLAKEI QHVNEEQETK KNSLEDKKWP NMCIEINRIL
810 820 830 840 850
LKSKDLLRQL PASNFNSLHF LIVHLKRVVD HAEENKMNSK NLGVIFGPSL
860 870 880 890 900
IRPRPTTAPI TISSLAEYSN QARLVEFLIT YSQKIFDGSL QPQDVMCSIG
910 920 930 940 950
VVDQGCFPKP LLSPEERDIE RSMKSLFFSS KEDIHTSESE SKIFERATSF
960 970 980 990 1000
EESERKQNAL GKCDACLSDK AQLLLDQEAE SASQKIEDGK TPKPLSLKSD
1010 1020 1030 1040 1050
RSTNNVERHT PRTKIRPVSL PVDRLLLASP PNERNGRNMG NVNLDKFCKN
1060 1070 1080 1090 1100
PAFEGVNRKD AATTVCSKFN GFDQQTLQKI QDKQYEQNSL TAKTTMIMPS
1110 1120 1130 1140 1150
ALQEKGVTTS LQISGDHSIN ATQPSKPYAE PVRSVREASE RRSSDSYPLA
1160 1170 1180 1190 1200
PVRAPRTLQP QHWTTFYKPH APIISIRGNE EKPASPSAAV PPGTDHDPHG
1210 1220 1230 1240 1250
LVVKSMPDPD KASACPGQAT GQPKEDSEEL GLPDVNPMCQ RPRLKRMQQF
1260
EDLEGEIPQF V
Length:1,261
Mass (Da):142,064
Last modified:February 5, 2008 - v2
Checksum:i3E46445EA5DA76C8
GO
Isoform 2 (identifier: Q52LW3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     383-393: EEANELYKVCV → TIFFFFICKLN
     394-1261: Missing.

Note: No experimental confirmation available.
Show »
Length:393
Mass (Da):45,194
Checksum:i6FF8EDA79AB7061C
GO

Sequence cautioni

The sequence AAH67839 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58M → I in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti160L → F in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti166S → Y in AAH67839 (PubMed:15489334).Curated1
Sequence conflicti212A → V in BAD92110 (Ref. 5) Curated1
Sequence conflicti386N → D in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti403L → V in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti446S → R in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti452D → G in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti675Q → L in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti720Q → L in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti856T → Q in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti991T → A in AAB81012 (PubMed:9305890).Curated1
Sequence conflicti1190V → C in AAB81012 (PubMed:9305890).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038552552S → C in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_0491451192P → L. Corresponds to variant dbSNP:rs11165091Ensembl.1
Natural variantiVAR_0385531255G → D2 PublicationsCorresponds to variant dbSNP:rs1999272Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031058383 – 393EEANELYKVCV → TIFFFFICKLN in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_031059394 – 1261Missing in isoform 2. 1 PublicationAdd BLAST868

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90920 mRNA Translation: AAB81012.1
AL162735 Genomic DNA No translation available.
CH471097 Genomic DNA Translation: EAW73052.1
CH471097 Genomic DNA Translation: EAW73051.1
BC022483 mRNA Translation: AAH22483.1
BC067839 mRNA Translation: AAH67839.1 Sequence problems.
BC093741 mRNA Translation: AAH93741.1
BC093767 mRNA Translation: AAH93767.1
AB208873 mRNA Translation: BAD92110.1
CCDSiCCDS748.1 [Q52LW3-1]
PIRiE59430
RefSeqiNP_001315593.1, NM_001328664.1 [Q52LW3-1]
NP_004806.3, NM_004815.3 [Q52LW3-1]
XP_011540741.1, XM_011542439.2 [Q52LW3-1]
UniGeneiHs.483238

Genome annotation databases

EnsembliENST00000260526; ENSP00000260526; ENSG00000137962 [Q52LW3-1]
ENST00000370217; ENSP00000359237; ENSG00000137962 [Q52LW3-2]
GeneIDi9411
KEGGihsa:9411
UCSCiuc001dqj.5 human [Q52LW3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRHG29_HUMAN
AccessioniPrimary (citable) accession number: Q52LW3
Secondary accession number(s): O15463
, Q59H86, Q5VYZ0, Q6NVX2, Q8TBI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome