Q52KZ6 (UB12A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12-A
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 12-A
Ubiquitin thioesterase 12-A
Ubiquitin-specific-processing protease 12-A

Gene names

Name:

usp12-a

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. USP12/USP46 subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Ubiquitin carboxyl-terminal hydrolase 12-A		PRO_0000378993
Sites
	Active site	48	1	Nucleophile By similarity
	Active site	317	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q52KZ6 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: AC83E979B65473D5	FASTA	370	42,865
	10 20 30 40 50 60 MEILMTVSRI ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC NSVLQALYFC 70 80 90 100 110 120 RPFREKVLAY KSQPRKKENL LTCLSDLFHS IATQKKKVGV IPPKKFITRL RKENELFDNY 130 140 150 160 170 180 MQQDAHEFLN YLLNTIADIL QEERKQEKQN GRIPNGNIDN ENNNSAPDPT WVHEIFQGTL 190 200 210 220 230 240 TNETRCLTCE TISSKDEDFL DLSVDVEQNT SITHCLRGFS NTETLCSEYK YYCEECRSKQ 250 260 270 280 290 300 EAHKRMKVKK LPMILALHLK RFKYMDQLHR YTKLSYRVVF PLELRLFNTS GDATNPDRMY 310 320 330 340 350 360 DLVAVVVHCG SGPNRGHYIA IVKSHDFWLL FDDDIVEKID AQAIEEFYGL TSDISKNSES 370 GYILFYQSRD

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC094130 mRNA. Translation: AAH94130.1.
RefSeq	NP_001089397.1. NM_001095928.1.
UniGene	Xl.52535.
3D structure databases
HSSP	HSSP built from PDB template 1NBF based on UniProtKB Q93009.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	734447.
KEGG	xla:734447.
Organism-specific databases
CTD	734447.
Xenbase	XB-GENE-1009335. usp12.
Phylogenomic databases
KO	K11842.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB12A_XENLA

Accession

Primary (citable) accession number: Q52KZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	May 24, 2005
Last modified:	July 11, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program