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Q52KN7

- CPE1B_XENLA

UniProt

Q52KN7 - CPE1B_XENLA

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Protein
Cytoplasmic polyadenylation element-binding protein 1-B
Gene
cpeb1-b
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation and early development. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In the absence of phosphorylation and in association with tacc3/maskin, also acts as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation. Requires zinc for RNA binding. Involved in the cell cycle progression from S phase into M phase By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi517 – 5171Zinc 1 By similarity
Metal bindingi520 – 5201Zinc 1 By similarity
Metal bindingi529 – 5291Zinc 2 By similarity
Metal bindingi534 – 5341Zinc 2 By similarity
Metal bindingi539 – 5391Zinc 1 By similarity
Metal bindingi542 – 5421Zinc 1 By similarity
Metal bindingi547 – 5471Zinc 2 By similarity
Metal bindingi555 – 5551Zinc 2 By similarity

GO - Molecular functioni

  1. mRNA 3'-UTR AU-rich region binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: InterPro
  4. translation repressor activity, nucleic acid binding Source: UniProtKB

GO - Biological processi

  1. cellular response to amino acid stimulus Source: UniProtKB
  2. cellular response to hypoxia Source: UniProtKB
  3. cellular response to insulin stimulus Source: UniProtKB
  4. mRNA processing Source: UniProtKB-KW
  5. negative regulation of cytoplasmic translation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor, Ribonucleoprotein

Keywords - Biological processi

mRNA processing, Translation regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic polyadenylation element-binding protein 1-B
Short name:
CPE-BP1-B
Short name:
CPE-binding protein 1-B
Short name:
CPEB-1-B
Gene namesi
Name:cpeb1-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6256188. cpeb1.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Membrane By similarity. Cytoplasmcytoskeletonspindle pole By similarity
Note: During mitosis localizes with tacc3/maskin and CPE-containing mRNAs to both spindle poles. Membrane-associated. Colocalizes with members of the polyadenylation and translation complex factors (cpsf, aplp1, tacc3/maskin, aurka, etc.), including CPE-containing RNAs By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. membrane Source: UniProtKB-SubCell
  3. microtubule organizing center Source: UniProtKB-SubCell
  4. nucleus Source: UniProtKB
  5. ribonucleoprotein complex Source: UniProtKB-KW
  6. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568Cytoplasmic polyadenylation element-binding protein 1-B
PRO_0000269258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei138 – 1381Phosphoserine; by cdk1 By similarity
Modified residuei174 – 1741Phosphoserine; by aurka By similarity
Modified residuei210 – 2101Phosphoserine; by cdk1 By similarity
Modified residuei248 – 2481Phosphoserine; by cdk1 By similarity

Post-translational modificationi

Ser-174 phosphorylation by aurka in immature oocytes is essential to trigger CPE-containing mRNA cytoplasmic polyadenylation and translation activation and the subsequent signaling events that result in meiotic progression. Ser-174 phosphorylation recruits the cleavage and polyadenylation specificity factor (cpsf1) into an active cytoplasmic polyadenylation complex. Ser-174 phosphorylation increases its affinity for cpsf1 and papd4/gld2. Heavily phosphorylated by CDK1 on serines late during oocyte maturation. Ser-210 phosphorylation is sufficient to target cpeb1 for degradation. Ser-174 phosphorylation oscillates with the cell cycle (phosphorylated at M phase, but not at S phase) and is necessary for S phase to M phase progression. Phosphorylation at Ser-174 may be promoted by aplp1 By similarity.
Ubiquitinated. Requires a PEST box and the proteasome pathway for destruction during oocyte maturation. Ser-210 phosphorylation triggers its destruction, an event important to allow the transition from metaphase I to metaphase II and cytokinesis in the early embryo By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Found in a complex with cpeb1, tacc3/maskin and eif4e; dissolution of this complex results in the binding of eif4e to eif4g and the translational activation of CPE-containing mRNAs. Found in a complex with cpeb1, cpsf1, the cytoplasmic poly(A) polymerase papd4/gld2 and sympk. Found in a mRNP complex with cpeb1, a guanine exchange factor xgef and mos mRNA. Interacts with cpsf1, papd4/gld2, tacc3/maskin, microtubules, sympk and xgef. Component of a ribonucleoprotein (RNP) complex, at least composed of cpeb1, lsm14b/rap55b, ddx6/Xp54, ybx2/frgy2, pat1/P100, eif4enif1/4E-T and eif4e1b. Interaction with ybx2/frgy2 is RNA-dependent. May interact with aplp1. Interaction with cpsf1 increases during meiotic maturation and is not mediated through RNA. Interaction with xgef is necessary for its early activating phosphorylation status By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ52KN7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini313 – 41098RRM 1
Add
BLAST
Domaini432 – 51382RRM 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1754Necessary for interaction with microtubules and localization at the mitotic apparatus By similarity
Regioni181 – 20828Necessary for degradation by the proteasome By similarity
Add
BLAST
Regioni182 – 19110Necessary for interaction with microtubules and localization at the mitotic apparatus By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi169 – 21042Ser-rich
Add
BLAST

Domaini

The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA By similarity.

Sequence similaritiesi

Belongs to the RRM CPEB family.

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG079080.
KOiK02602.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q52KN7-1 [UniParc]FASTAAdd to Basket

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MAFPLKDDLG RAKDCWGCSS DTPALSTCSN ADIFRRINAM LDNSLDFTGV    50
CTTPNTKGKC EHLQDYPDTE GAAASRMLFS TSHEPLPRGL PDTNDLCLGL 100
QSLSLTGWDR PWSTQDSEAG GQSSTPTAAQ SVFSMLNSPM GKPSPLGFLP 150
LDPIGSDLVE KYPTHLLRSS RFDSRSILDS RSSSPSDSDT SGFSSGSDHL 200
SDLISSLRIS PPLPFLPLGS GISRDPLRLG VGSRLDQDHA ALAAATASPL 250
GITKRWPGTS VWPSWDLLDS ADDPFSIERE ARLHRQAAAV NEATCTWSGQ 300
LPPRNYKNPV YSCKVFLGGV PWDITETGLI NTFRVFGALS VEWPGKDGKH 350
PRCPPKGNMP KGYVYLVFES EKSVRALLQA CTQDLLSQDG LSEHYFKMSS 400
RRMRCKEVQV IPWVLADSNF VRSPSQRLDP SKTVFVGALH GMLNAEALAS 450
IMNDLFGGVV YAGIDTDKHK YPIGSGRVTF NNQRSYLKAV SAAFVEIKTA 500
KFTKKVQIDP YLEDSVCQVC NAQPGPFFCR DQVCFKYFCR SCWHWQHSME 550
ILRHHRPLMR NQKSRDSS 568
Length:568
Mass (Da):62,632
Last modified:May 24, 2005 - v1
Checksum:i109829643720D8F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC094261 mRNA. Translation: AAH94261.1.
RefSeqiNP_001089420.1. NM_001095951.1.
UniGeneiXl.29059.

Genome annotation databases

GeneIDi734470.
KEGGixla:734470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC094261 mRNA. Translation: AAH94261.1 .
RefSeqi NP_001089420.1. NM_001095951.1.
UniGenei Xl.29059.

3D structure databases

ProteinModelPortali Q52KN7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 734470.
KEGGi xla:734470.

Organism-specific databases

CTDi 734470.
Xenbasei XB-GENE-6256188. cpeb1.

Phylogenomic databases

HOVERGENi HBG079080.
KOi K02602.

Family and domain databases

Gene3Di 3.30.70.330. 3 hits.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Oocyte.

Entry informationi

Entry nameiCPE1B_XENLA
AccessioniPrimary (citable) accession number: Q52KN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 24, 2005
Last modified: January 22, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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