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Q52KN7

- CPE1B_XENLA

UniProt

Q52KN7 - CPE1B_XENLA

Protein

Cytoplasmic polyadenylation element-binding protein 1-B

Gene

cpeb1-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation and early development. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In the absence of phosphorylation and in association with tacc3/maskin, also acts as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation. Requires zinc for RNA binding. Involved in the cell cycle progression from S phase into M phase By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi517 – 5171Zinc 1By similarity
    Metal bindingi520 – 5201Zinc 1By similarity
    Metal bindingi529 – 5291Zinc 2By similarity
    Metal bindingi534 – 5341Zinc 2By similarity
    Metal bindingi539 – 5391Zinc 1By similarity
    Metal bindingi542 – 5421Zinc 1By similarity
    Metal bindingi547 – 5471Zinc 2By similarity
    Metal bindingi555 – 5551Zinc 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. mRNA 3'-UTR AU-rich region binding Source: UniProtKB
    3. nucleotide binding Source: InterPro
    4. translation repressor activity, nucleic acid binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to amino acid stimulus Source: UniProtKB
    2. cellular response to hypoxia Source: UniProtKB
    3. cellular response to insulin stimulus Source: UniProtKB
    4. mRNA processing Source: UniProtKB-KW
    5. negative regulation of cytoplasmic translation Source: UniProtKB

    Keywords - Molecular functioni

    Activator, Repressor, Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, Translation regulation

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytoplasmic polyadenylation element-binding protein 1-B
    Short name:
    CPE-BP1-B
    Short name:
    CPE-binding protein 1-B
    Short name:
    CPEB-1-B
    Gene namesi
    Name:cpeb1-b
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-6256188. cpeb1.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Membrane By similarity. Cytoplasmcytoskeletonspindle pole By similarity
    Note: During mitosis localizes with tacc3/maskin and CPE-containing mRNAs to both spindle poles. Membrane-associated. Colocalizes with members of the polyadenylation and translation complex factors (cpsf, aplp1, tacc3/maskin, aurka, etc.), including CPE-containing RNAs By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. membrane Source: UniProtKB-SubCell
    3. microtubule organizing center Source: UniProtKB-SubCell
    4. nucleus Source: UniProtKB
    5. ribonucleoprotein complex Source: UniProtKB-KW
    6. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 568568Cytoplasmic polyadenylation element-binding protein 1-BPRO_0000269258Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei138 – 1381Phosphoserine; by cdk1By similarity
    Modified residuei174 – 1741Phosphoserine; by aurkaBy similarity
    Modified residuei210 – 2101Phosphoserine; by cdk1By similarity
    Modified residuei248 – 2481Phosphoserine; by cdk1By similarity

    Post-translational modificationi

    Ser-174 phosphorylation by aurka in immature oocytes is essential to trigger CPE-containing mRNA cytoplasmic polyadenylation and translation activation and the subsequent signaling events that result in meiotic progression. Ser-174 phosphorylation recruits the cleavage and polyadenylation specificity factor (cpsf1) into an active cytoplasmic polyadenylation complex. Ser-174 phosphorylation increases its affinity for cpsf1 and papd4/gld2. Heavily phosphorylated by CDK1 on serines late during oocyte maturation. Ser-210 phosphorylation is sufficient to target cpeb1 for degradation. Ser-174 phosphorylation oscillates with the cell cycle (phosphorylated at M phase, but not at S phase) and is necessary for S phase to M phase progression. Phosphorylation at Ser-174 may be promoted by aplp1 By similarity.By similarity
    Ubiquitinated. Requires a PEST box and the proteasome pathway for destruction during oocyte maturation. Ser-210 phosphorylation triggers its destruction, an event important to allow the transition from metaphase I to metaphase II and cytokinesis in the early embryo By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Found in a complex with cpeb1, tacc3/maskin and eif4e; dissolution of this complex results in the binding of eif4e to eif4g and the translational activation of CPE-containing mRNAs. Found in a complex with cpeb1, cpsf1, the cytoplasmic poly(A) polymerase papd4/gld2 and sympk. Found in a mRNP complex with cpeb1, a guanine exchange factor xgef and mos mRNA. Interacts with cpsf1, papd4/gld2, tacc3/maskin, microtubules, sympk and xgef. Component of a ribonucleoprotein (RNP) complex, at least composed of cpeb1, lsm14b/rap55b, ddx6/Xp54, ybx2/frgy2, pat1/P100, eif4enif1/4E-T and eif4e1b. Interaction with ybx2/frgy2 is RNA-dependent. May interact with aplp1. Interaction with cpsf1 increases during meiotic maturation and is not mediated through RNA. Interaction with xgef is necessary for its early activating phosphorylation status By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ52KN7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini313 – 41098RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini432 – 51382RRM 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni172 – 1754Necessary for interaction with microtubules and localization at the mitotic apparatusBy similarity
    Regioni181 – 20828Necessary for degradation by the proteasomeBy similarityAdd
    BLAST
    Regioni182 – 19110Necessary for interaction with microtubules and localization at the mitotic apparatusBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi169 – 21042Ser-richAdd
    BLAST

    Domaini

    The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.By similarity

    Sequence similaritiesi

    Belongs to the RRM CPEB family.Curated
    Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG079080.
    KOiK02602.

    Family and domain databases

    Gene3Di3.30.70.330. 3 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q52KN7-1 [UniParc]FASTAAdd to Basket

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    MAFPLKDDLG RAKDCWGCSS DTPALSTCSN ADIFRRINAM LDNSLDFTGV    50
    CTTPNTKGKC EHLQDYPDTE GAAASRMLFS TSHEPLPRGL PDTNDLCLGL 100
    QSLSLTGWDR PWSTQDSEAG GQSSTPTAAQ SVFSMLNSPM GKPSPLGFLP 150
    LDPIGSDLVE KYPTHLLRSS RFDSRSILDS RSSSPSDSDT SGFSSGSDHL 200
    SDLISSLRIS PPLPFLPLGS GISRDPLRLG VGSRLDQDHA ALAAATASPL 250
    GITKRWPGTS VWPSWDLLDS ADDPFSIERE ARLHRQAAAV NEATCTWSGQ 300
    LPPRNYKNPV YSCKVFLGGV PWDITETGLI NTFRVFGALS VEWPGKDGKH 350
    PRCPPKGNMP KGYVYLVFES EKSVRALLQA CTQDLLSQDG LSEHYFKMSS 400
    RRMRCKEVQV IPWVLADSNF VRSPSQRLDP SKTVFVGALH GMLNAEALAS 450
    IMNDLFGGVV YAGIDTDKHK YPIGSGRVTF NNQRSYLKAV SAAFVEIKTA 500
    KFTKKVQIDP YLEDSVCQVC NAQPGPFFCR DQVCFKYFCR SCWHWQHSME 550
    ILRHHRPLMR NQKSRDSS 568
    Length:568
    Mass (Da):62,632
    Last modified:May 24, 2005 - v1
    Checksum:i109829643720D8F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC094261 mRNA. Translation: AAH94261.1.
    RefSeqiNP_001089420.1. NM_001095951.1.
    UniGeneiXl.29059.

    Genome annotation databases

    GeneIDi734470.
    KEGGixla:734470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC094261 mRNA. Translation: AAH94261.1 .
    RefSeqi NP_001089420.1. NM_001095951.1.
    UniGenei Xl.29059.

    3D structure databases

    ProteinModelPortali Q52KN7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 734470.
    KEGGi xla:734470.

    Organism-specific databases

    CTDi 734470.
    Xenbasei XB-GENE-6256188. cpeb1.

    Phylogenomic databases

    HOVERGENi HBG079080.
    KOi K02602.

    Family and domain databases

    Gene3Di 3.30.70.330. 3 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Oocyte.

    Entry informationi

    Entry nameiCPE1B_XENLA
    AccessioniPrimary (citable) accession number: Q52KN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3