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Protein

H/ACA ribonucleoprotein complex non-core subunit NAF1

Gene

Naf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex non-core subunit NAF1
Gene namesi
Name:Naf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1306802. Naf1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003156391 – 457H/ACA ribonucleoprotein complex non-core subunit NAF1Add BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei280PhosphoserineCombined sources1
Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ52KK4.
PRIDEiQ52KK4.

PTM databases

iPTMnetiQ52KK4.
PhosphoSitePlusiQ52KK4.

Interactioni

Subunit structurei

During assembly of the complex, component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs) which contains NOLA2/NHP2, NOLA3/NOP10, NAF1 and DKC1/NOLA4. Interacts directly with DKC1/NOLA4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031163.

Structurei

3D structure databases

ProteinModelPortaliQ52KK4.
SMRiQ52KK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi26 – 77Pro-richAdd BLAST52
Compositional biasi82 – 113Ser-richAdd BLAST32
Compositional biasi354 – 445Pro-richAdd BLAST92

Sequence similaritiesi

Belongs to the NAF1 family.Curated

Phylogenomic databases

eggNOGiKOG2236. Eukaryota.
ENOG4111RRE. LUCA.
HOGENOMiHOG000214085.
HOVERGENiHBG108169.
InParanoidiQ52KK4.
KOiK14763.
PhylomeDBiQ52KK4.

Family and domain databases

InterProiView protein in InterPro
IPR007504. H/ACA_rnp_Gar1/Naf1.
IPR009000. Transl_B-barrel.
PfamiView protein in Pfam
PF04410. Gar1. 1 hit.
SUPFAMiSSF50447. SSF50447. 1 hit.

Sequencei

Sequence statusi: Complete.

Q52KK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVVEAAAQL QTLKFGGSGP GSAASPPPEE RPDAEQAEQA PPAGEQPPPA
60 70 80 90 100
PSSDAGENPP PPSGPCATAP DAAEPLPELQ DSDSSDSDSD SETDSDSSSS
110 120 130 140 150
SSSSSSSSSS CGSLPPVLSD GEEDVQVEKE NKNFPLKTKD ELLLNELPSV
160 170 180 190 200
EELTVTLPED IALKPLGKVS SIIEQLVIIE SMTNIPPVNE DTVIFKSDRQ
210 220 230 240 250
AAGKIFEIFG PVAHPFYVLR FNSSEHIESK GIKIKDTMYF APSMKDFTQY
260 270 280 290 300
IFTEKLKQDR GSDASWKNDQ EPPPEALDFS DDEKEKEAKQ RKKSQIQGRK
310 320 330 340 350
KLKSELNESG EDFGEVHQNW NANSSSEHSK GYHNREFTRG FPRGRYSRGS
360 370 380 390 400
HGRPPPQQYY NSDPMASQES LGFPPQRQDN PVMPHYPFPP PMFDMHNFPL
410 420 430 440 450
PPPPPPPPTV SMGWAAPSMT SHPVLNLPYS LPPPPLPPPP PPPSPGESNS

SHFGSYY
Length:457
Mass (Da):49,764
Last modified:May 24, 2005 - v1
Checksum:i08D131A43D54250D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC094302 mRNA. Translation: AAH94302.1.
RefSeqiNP_001019943.1. NM_001024772.1.
UniGeneiRn.162269.

Genome annotation databases

GeneIDi306387.
KEGGirno:306387.
UCSCiRGD:1306802. rat.

Similar proteinsi

Entry informationi

Entry nameiNAF1_RAT
AccessioniPrimary (citable) accession number: Q52KK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 24, 2005
Last modified: August 30, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families