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Q52I78 (NAMPT_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nicotinamide phosphoribosyltransferase

Short name=NAmPRTase
Short name=Nampt
EC=2.4.2.12
Alternative name(s):
Pre-B-cell colony-enhancing factor 1 homolog
Short name=PBEF
Visfatin
Gene names
Name:NAMPT
Synonyms:PBEF1
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function By similarity.

Catalytic activity

Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Secreted By similarity. Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum By similarity.

Sequence similarities

Belongs to the NAPRTase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Nicotinamide phosphoribosyltransferase
PRO_0000205865

Regions

Region311 – 3133Nicotinamide ribonucleotide binding By similarity
Region353 – 3542Nicotinamide ribonucleotide binding By similarity

Sites

Binding site1961Diphosphate By similarity
Binding site2191Nicotinamide ribonucleotide By similarity
Binding site2471Diphosphate By similarity
Binding site3111Diphosphate By similarity
Binding site3841Nicotinamide ribonucleotide; via amide nitrogen By similarity
Binding site3921Nicotinamide ribonucleotide By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q52I78 [UniParc].

Last modified January 24, 2006. Version 2.
Checksum: EC9CF05026B4793E

FASTA49155,374
        10         20         30         40         50         60 
MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKIR KVKYEETVFY 

        70         80         90        100        110        120 
GLQYILNKYL KGKVVTKEKI QEAKEVYKEH FQDDVFNEKG WNYILEKYDG HLPIEVKAVP 

       130        140        150        160        170        180 
EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS 

       190        200        210        220        230        240 
GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY 

       250        260        270        280        290        300 
SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV 

       310        320        330        340        350        360 
SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT 

       370        380        390        400        410        420 
LQEIVEGMKQ KKWSIENIAF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD 

       430        440        450        460        470        480 
PNKRSKKGRL SLHRTPGGNF VTLEEGKGDL EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA 

       490 
QLNIELEAAP H 

« Hide

References

[1]"Cloning and expression of porcine visfatin."
Liu B.-H., Ding S.-T.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Chen H., Yang Z.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ020218 mRNA. Translation: AAY89036.1.
DQ001974 mRNA. Translation: AAY18209.2.
RefSeqNP_001026963.1. NM_001031793.2.
XP_005667778.1. XM_005667721.1.
UniGeneSsc.22083.

3D structure databases

ProteinModelPortalQ52I78.
SMRQ52I78. Positions 8-483.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000016366.

Proteomic databases

PRIDEQ52I78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000016815; ENSSSCP00000016366; ENSSSCG00000015435.
GeneID595123.
KEGGssc:595123.

Organism-specific databases

CTD10135.

Phylogenomic databases

eggNOGCOG1488.
GeneTreeENSGT00390000006647.
HOGENOMHOG000216546.
HOVERGENHBG000336.
KOK03462.
OMAKKFPITE.
OrthoDBEOG7PGDQH.
TreeFamTF333530.

Enzyme and pathway databases

UniPathwayUPA00253; UER00890.

Family and domain databases

InterProIPR007229. Nic_PRibTrfase-Fam.
IPR016471. Nicotinamide_PRibTrfase.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERPTHR11098:SF2. PTHR11098:SF2. 1 hit.
PfamPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFPIRSF005943. NMPRT. 1 hit.
SUPFAMSSF51690. SSF51690. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNAMPT_PIG
AccessionPrimary (citable) accession number: Q52I78
Secondary accession number(s): Q3I6K8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways