Reviewed, UniProtKB/Swiss-Prot Q52EB3 (ATG1_MAGGR)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ATG1
    EC=2.7.11.1
Alternative name(s):
    Autophagy-related protein 1

Gene names

Name:	ATG1
ORF Names:	MGG_06393

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	982 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine/threonine protein kinase probably involved in the cytoplasm to vacuole transport (Cvt) and in autophagy, where it may be required for the formation of autophagosomes. Autophagy is essential to fungal development, production of appressorium turgor, and pathogenicity in rice blast disease. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Uniformly detected in conidia, mycelia and appressoria (at protein level). Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 982	982	Serine/threonine-protein kinase ATG1		PRO_0000085648
Regions
	Domain	19 – 324	306	Protein kinase
	Nucleotide binding	25 – 33	9	ATP By similarity
Sites
	Active site	162	1	Proton acceptor By similarity
	Binding site	48	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q52EB3-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 9811E0BC78307638	FASTA	982	107,443
	10 20 30 40 50 60 MADRSARRAR PGDDSVGQFV IGAEIGKGSF AQVYMGKHKV SGAAVAIKSV ELARLNKKLK 70 80 90 100 110 120 ENLYGEINIL KTLRHPHIVA LHDCVESATH INLMMEYCEL GDLSLFIKKR EKLSTNPATH 130 140 150 160 170 180 DMARKYPNVP NSGLNEVVIR HFLKQLSSAL KFLRESNLVH RDVKPQNLLL LPSPEFREVN 190 200 210 220 230 240 KLARPILTAS QDSLVPVAGL ASLPMLKLAD FGFARVLPST SLAETLCGSP LYMAPEILRY 250 260 270 280 290 300 ERYDAKADLW SVGTVLFEMI VGRPPFRASN HVELLRKIEA AEDVIKFPRE TTISSEMKGL 310 320 330 340 350 360 TRALLKRNPV ERISFENFFA HPVIISSIPG LVEDDIPKPE ASEQRSSSKD TRAASKSDDP 370 380 390 400 410 420 IASPRKYSFR RHPTDNDQIR DQFRRVEPPS SAAESAPSRQ TSAFSGIAAE ARKQAAAEAS 430 440 450 460 470 480 ARTGQSSRNE PGDNLVPRRP QAQPSTSAPS KPGLYEERRR GISNASLNRS NRESSSPTSA 490 500 510 520 530 540 ALANDSARAP PQQTSRRVQA EEREKAAQDV AFERDYVVVE KKHVEVNAFA DEMAANPRLG 550 560 570 580 590 600 GQGTPLSPKS GQIVRRATQQ GNPTSTTGAI PAAPSRTMQI AQGTQRHYHE RGTSLSASPG 610 620 630 640 650 660 STASFITKAI QDASLRLLGI KYPAGLTKGA SPPELYNPYP AYPTPSPPVG LISSGKQSTP 670 680 690 700 710 720 VDEDARVAQL IEEHATRSDV VYGFAEVKYK QLVPLAPSAE HGLGGTTLED MPTGEEDVLT 730 740 750 760 770 780 VEAIVSLSEE ALVLYVKALT LLAKSMDIAS LWWARKNRGD QANNALSSTR DSVNTQTLSL 790 800 810 820 830 840 RINSAVQWVR SRFNEVLEKA EVVRLRLMDA QKRLPDDHPS HPSNHPQGSE SVNGASAEGV 850 860 870 880 890 900 FLTVGVTAEK LMYDRALEMS RTAAINEITN EDLAGCEISY VTAIRMLEAV LDNDEDAPKR 910 920 930 940 950 960 RLSANMDDSG GDGEDGHAEI NADDQQAVQK MIHMIRSRLT SVRSKVRMIS NASKAQQQSQ 970 980 PQSLIRRRSG DVTPRSVPSY SS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Involvement of a Magnaporthe grisea serine/threonine kinase gene, MgATG1, in appressorium turgor and pathogenesis." Liu X.-H., Lu J.-P., Zhang L., Dong B., Min H., Lin F.-C. Eukaryot. Cell 6:997-1005(2007) [PubMed: 17416896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION. Strain: Guyane 11.
[2]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ224381 mRNA. Translation: ABB46201.1. CH476833 Genomic DNA. Translation: EDK00687.1.
RefSeq	XP_369878.1.
UniGene	Mgr.9939
3D structure databases
SMR	Q52EB3. Positions 15-324.
ModBase	Search...
Genome annotation databases
GeneID	2684548.
KEGG	mgr:MGG_06393.
NMPDR	fig|242507.1.peg.10456.
Phylogenomic databases
OMA	KYNAKAD.
OrthoDB	EOG98D2C4.
PhylomeDB	Q52EB3.
Enzyme and pathway databases
BRENDA	2.7.11.1. 5953.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR020656. Ser/Thr_prot_kinase_ATG1. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF39. Ser/Thr_prot_kinase_ATG1. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG1_MAGGR

Accession

Primary (citable) accession number: Q52EB3
Secondary accession number(s): Q2Q439

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)