Reviewed, UniProtKB/Swiss-Prot Q52995 (ECHH_RHIME)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase
    EC=4.2.1.17

Gene names

Name:	fadB1
Ordered Locus Names:	R00366
ORF Names:	SMc01153

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Probable enoyl-CoA hydratase		PRO_0000109344
Experimental info
	Sequence conflict	129	1	Q → L in AAA91095. Ref.1
	Sequence conflict	196	1	I → T in AAA91095. Ref.1
	Sequence conflict	220	1	A → T in AAA91095. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q52995-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 971970C6C6E780A7	FASTA	257	27,472
	10 20 30 40 50 60 MSYETLLVET QGRVGLITLN RPQALNALNA VLMRELDAAL KAFDADRAVG AIVLAGSEKA 70 80 90 100 110 120 FAAGADIKEM QGLDFVDGYL ADFLGGWEHV ANARKPMIAA VSGFALGGGC ELAMMCDFII 130 140 150 160 170 180 ASETAKFGQP EITLGVIPGM GGSQRLTRAV GKAKAMDLIL TGRMMDAAEA ERSGLVSRVV 190 200 210 220 230 240 APDRLLEEAL GAAEKIASFS LPAAMMAKEA VNRSLELTLA EGLRFERRLF QSLFATEDQK 250 EGMAAFVAKR KAEFKHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The dnaA gene of Rhizobium meliloti lies within an unusual gene arrangement." Margolin W., Bramhill D., Long S.R. J. Bacteriol. 177:2892-2900(1995) [PubMed: 7751302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L39265 Genomic DNA. Translation: AAA91095.1. AL591688 Genomic DNA. Translation: CAC41803.1.
RefSeq	NP_384472.1.
3D structure databases
HSSP	HSSP built from PDB template 1MJ3 based on UniProtKB P14604.
ModBase	Search...
Genome annotation databases
GeneID	1232000.
GenomeReviews	Gene locus R00366 in contig AL591688_GR.
KEGG	sme:SMc01153.
NMPDR	fig|266834.1.peg.1660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q52995.
OMA	LIVETRG.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC01153-MON.
BRENDA	4.2.1.17. 142.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECHH_RHIME

Accession

Primary (citable) accession number: Q52995

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 3, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)