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Protein

Probable K(+)/H(+) antiporter subunit A/B

Gene

phaAB

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Part of a K+ efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-2979-MONOMER.

Protein family/group databases

TCDBi2.A.63.1.1. the monovalent cation (k(+) or na(+)):proton antiporter-3 (cpa3) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable K(+)/H(+) antiporter subunit A/B
Alternative name(s):
pH adaptation potassium efflux system protein A/B
Short name:
Pha system subunit A/B
Gene namesi
Name:phaAB
Synonyms:phaA, phaA1, phaB
Ordered Locus Names:R02910
ORF Names:SMc03179
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 4818HelicalSequence analysisAdd
BLAST
Transmembranei63 – 8523HelicalSequence analysisAdd
BLAST
Transmembranei106 – 12823HelicalSequence analysisAdd
BLAST
Transmembranei138 – 16023HelicalSequence analysisAdd
BLAST
Transmembranei162 – 18120HelicalSequence analysisAdd
BLAST
Transmembranei191 – 21323HelicalSequence analysisAdd
BLAST
Transmembranei233 – 25523HelicalSequence analysisAdd
BLAST
Transmembranei270 – 29223HelicalSequence analysisAdd
BLAST
Transmembranei299 – 32123HelicalSequence analysisAdd
BLAST
Transmembranei336 – 35823HelicalSequence analysisAdd
BLAST
Transmembranei389 – 41123HelicalSequence analysisAdd
BLAST
Transmembranei442 – 46423HelicalSequence analysisAdd
BLAST
Transmembranei488 – 51023HelicalSequence analysisAdd
BLAST
Transmembranei530 – 55223HelicalSequence analysisAdd
BLAST
Transmembranei604 – 62118HelicalSequence analysisAdd
BLAST
Transmembranei636 – 65318HelicalSequence analysisAdd
BLAST
Transmembranei660 – 68223HelicalSequence analysisAdd
BLAST
Transmembranei686 – 70823HelicalSequence analysisAdd
BLAST
Transmembranei729 – 75123HelicalSequence analysisAdd
BLAST
Transmembranei788 – 80720HelicalSequence analysisAdd
BLAST
Transmembranei846 – 86823HelicalSequence analysisAdd
BLAST
Transmembranei878 – 90023HelicalSequence analysisAdd
BLAST
Transmembranei913 – 93523HelicalSequence analysisAdd
BLAST
Transmembranei955 – 97723HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 999999Probable K(+)/H(+) antiporter subunit A/BPRO_0000217075Add
BLAST

Interactioni

Subunit structurei

May form a heterooligomeric complex that consists of six subunits: PhaAB, PhaC, PhaD, PhaE, PhaF and PhaG.

Protein-protein interaction databases

STRINGi266834.SMc03179.

Structurei

3D structure databases

ProteinModelPortaliQ52978.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the CPA3 antiporters (TC 2.A.63) subunit A family. [View classification]Curated
In the C-terminal section; belongs to the CPA3 antiporters (TC 2.A.63) subunit B family. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D65. Bacteria.
COG1009. LUCA.
COG2111. LUCA.
HOGENOMiHOG000066205.
KOiK05559.
OMAiNNTILMY.

Family and domain databases

InterProiIPR005281. CPA3_sub_B.
IPR025383. DUF4040.
IPR007182. MnhB.
IPR005663. MrpA/MnhA1/PhaAB.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
[Graphical view]
PfamiPF13244. DUF4040. 1 hit.
PF04039. MnhB. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00940. 2a6301s01. 1 hit.
TIGR00943. 2a6301s02. 1 hit.

Sequencei

Sequence statusi: Complete.

Q52978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRPASVLAG PKSRPPIHSQ GDKTIEYAEK LLSVFILVPF AGSLIAIFFP
60 70 80 90 100
SDQRGAISWF AGAIALVCFL VTAGLYPYVA SGGVLHYRID WVPELGLNFT
110 120 130 140 150
LRMDGFAWLF SALITAIGVL VALYARYYMA EEDPVPRFFA LFLAFMGSML
160 170 180 190 200
GVVLSGNLIL LAVFWELTSI VSFLLIGYWH HNAHARDGAR MALTITGTGG
210 220 230 240 250
LAMFVGLIII GKIVGSYELD AVLASGDAIR NHPLYGTVLV LVLLGALTKS
260 270 280 290 300
AQFPFHFWLP HAMAAPTPVS AYLHSATMVK AGVFLLVRFW PVMAGTEAWF
310 320 330 340 350
WIVGLAGLTT LLLGAYFAIF QQDLKGLLAY STISHLGLIT VLLSLGSPLA
360 370 380 390 400
AVAAVFHIVN HATFKASLFM AAGIIDHESG TRDIRRLGGL FHFMPITATL
410 420 430 440 450
AMVASAAMAG VPLLNGFLSK EMFFAEAIET HLVNPLDTVT PYVATIAGMF
460 470 480 490 500
AVTYSLRFIH GVFFGRPPAD LPRKPHEPPR WMRAPLDFLV LACLVVGIIP
510 520 530 540 550
AQTIGPFLHT AVLSVLREGT PDYSLSVWHG WNIPLIMSFV ALSGGIGLYF
560 570 580 590 600
LMRSYLATAV EGPPVFRLLQ GQRIFERVLV TLSWKWARWL EQRLGTRRLQ
610 620 630 640 650
PQMRLLVFLA LAAGASPLLL GNFELPPLVI RGIDPAFALL WAIGIACAIG
660 670 680 690 700
SAYQAKFHRL ASLVLLGGAG LVTCITFVWL SAPDLAVTQL LVEIVTTVLI
710 720 730 740 750
LLGLRWLPKR IEEPVAAEDI SIRVRLRRLR DLLLAIGAGG GMMLIAYTVM
760 770 780 790 800
TRPLPETIAS YFLERAYREG GGTNVVNVIL VDFRGFDTLG EIAVLCIVAL
810 820 830 840 850
TVFALLLRFR PQSDSLEAPE QQKVQNAFDD DHPDRAAGDS VAEYLFIPAV
860 870 880 890 900
IMRWMFPVTG MLAAFLFLRG HDLPGGGFAA GIAMSIGFIL QYMSGGTRWV
910 920 930 940 950
EERLRIHPLR WMSIGLLVAT ATGVGSWFFG YPFLTSHAQY ASLPVVGKFP
960 970 980 990
LASAILFDLG VFSLVLGATV LILIALAHQS VRAPRAHAKA ARSDKEAVR
Length:999
Mass (Da):108,790
Last modified:April 13, 2004 - v2
Checksum:i9857B829580C2286
GO

Sequence cautioni

The sequence CAA63735 differs from that shown. Reason: Frameshift at position 718. Produces two separate ORFs named phaA and phaB.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221A → V in CAA63734 (PubMed:9680201).Curated
Sequence conflicti188 – 1881G → A in CAA63734 (PubMed:9680201).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93358 Genomic DNA. Translation: CAA63734.1. Frameshift.
X93358 Genomic DNA. Translation: CAA63735.1. Frameshift.
AL591688 Genomic DNA. Translation: CAC47489.1.
AF465791 Genomic DNA. Translation: AAL76258.1.
RefSeqiNP_387016.1. NC_003047.1.
WP_010970284.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC47489; CAC47489; SMc03179.
GeneIDi1234587.
KEGGisme:SMc03179.
PATRICi23635404. VBISinMel96828_4431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93358 Genomic DNA. Translation: CAA63734.1. Frameshift.
X93358 Genomic DNA. Translation: CAA63735.1. Frameshift.
AL591688 Genomic DNA. Translation: CAC47489.1.
AF465791 Genomic DNA. Translation: AAL76258.1.
RefSeqiNP_387016.1. NC_003047.1.
WP_010970284.1. NC_003047.1.

3D structure databases

ProteinModelPortaliQ52978.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc03179.

Protein family/group databases

TCDBi2.A.63.1.1. the monovalent cation (k(+) or na(+)):proton antiporter-3 (cpa3) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC47489; CAC47489; SMc03179.
GeneIDi1234587.
KEGGisme:SMc03179.
PATRICi23635404. VBISinMel96828_4431.

Phylogenomic databases

eggNOGiENOG4105D65. Bacteria.
COG1009. LUCA.
COG2111. LUCA.
HOGENOMiHOG000066205.
KOiK05559.
OMAiNNTILMY.

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-2979-MONOMER.

Family and domain databases

InterProiIPR005281. CPA3_sub_B.
IPR025383. DUF4040.
IPR007182. MnhB.
IPR005663. MrpA/MnhA1/PhaAB.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
[Graphical view]
PfamiPF13244. DUF4040. 1 hit.
PF04039. MnhB. 1 hit.
PF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00940. 2a6301s01. 1 hit.
TIGR00943. 2a6301s02. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPHAAB_RHIME
AccessioniPrimary (citable) accession number: Q52978
Secondary accession number(s): Q52979, Q8RTE9, Q92LV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.