Q52915 (CYA2_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase 2
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase 2
Adenylyl cyclase 2

Gene names

Name:	cya2
Ordered Locus Names:	R01190
ORF Names:	SMc00621

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays essential roles in regulation of cellular metabolism by catalyzing the synthesis of a second messenger, cAMP.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Adenylate cyclase 2		PRO_0000195745
Regions
	Domain	157 – 286	130	Guanylate cyclase
Sites
	Metal binding	162	1	Magnesium By similarity
	Metal binding	206	1	Magnesium By similarity
Experimental info
	Sequence conflict	179	1	Q → H in CAA56916. Ref.1
	Sequence conflict	302	1	L → R in CAA56916. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q52915 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 486A0B0C43378E37	FASTA	363	39,902
	10 20 30 40 50 60 MRWRFSTLEI ILLLLVVAGS GMAYAWVVYG GGGLIGATYA LFMCMPILAF ERHIIFRRLY 70 80 90 100 110 120 RRIHGSPTPA FLLSSLAVYF IFVNVGYAAA GLLLHVAGVM RESRTDAMLP SLNVLVYALA 130 140 150 160 170 180 TSGPIIFVLR VRELLGRDVF LSLLTGRYRK PVQEERVFLF IDLAGSTSLA ERFGDLRMQE 190 200 210 220 230 240 YLGKLFAAMA DPVLRYGGSI DDYVGDAAVI TWPYDRAVAD AACIRCVFDI LEQIEADAHR 250 260 270 280 290 300 WQKDYGEVPR LRAALHGGTI VAAEIGVDKH KITYFGDTVN TTARLEGLCR TLNRQVLISA 310 320 330 340 350 360 DLLRRLRPPV FVRAEDLGEH EVKGRGQKLA VLSLTAGSLS GDGATEPAGE TVRSPAAEAF TSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the second adenylate cyclase gene (cya2) from Rhizobium meliloti F34: sequence similarity to eukaryotic cyclases." Archdeacon J., Talty J., Boesten B., Danchin A., O'Gara F. FEMS Microbiol. Lett. 128:177-184(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: F34.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80991 Genomic DNA. Translation: CAA56916.1. AL591688 Genomic DNA. Translation: CAC45769.1.
PIR	S60684.
RefSeq	NP_385296.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q52915.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc00621.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC45769; CAC45769; SMc00621.
GeneID	1232834.
KEGG	sme:SMc00621.
PATRIC	23631681. VBISinMel96828_2601.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2114.
HOGENOM	HOG000295398.
KO	K01768.
OMA	RYHRPVE.
ProtClustDB	CLSK881495.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-1216-MONOMER.
Family and domain databases
Gene3D	3.30.70.1230. 1 hit.
InterPro	IPR001054. A/G_cyclase. [Graphical view]
Pfam	PF00211. Guanylate_cyc. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit.
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYA2_RHIME

Accession

Primary (citable) accession number: Q52915

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program