Q52883 (CHEB1_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	R00642
ORF Names:	SMc03010

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Miscellaneous	R.meliloti does not have a group 2 operon. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158019
Regions
	Domain	6 – 123	118	Response regulatory
	Domain	152 – 344	193	CheB-type methylesterase
Sites
	Active site	164	1	By similarity
	Active site	190	1	By similarity
	Active site	286	1	By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	31	1	T → S in AAA86677. Ref.1
	Sequence conflict	114	1	T → P in AAA86677. Ref.1
	Sequence conflict	152	1	S → Y in AAA86677. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q52883 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 042C3986FC12FBA9	FASTA	349	36,908
	10 20 30 40 50 60 MSAPARVLVV DDSATMRGLI SAVLNADPDI TVVGQAADAL EARQAIKQLD PDVVTLDIEM 70 80 90 100 110 120 PNMNGLEFLD KIMRLRPMPV IMVSTLTHRG AEATIAALEI GAFDCVGKPQ PGDTHPFRDL 130 140 150 160 170 180 ADKVKAAARS QRKSMITSNR AAAPAATAVS DSRAGRKIVA IGASTGGVEA LITVLQKFPA 190 200 210 220 230 240 NCPPTVITQH MPHTFTKSFA ERLNRLCAPT VQEATDGARL EVGRVYLAPG GERHLEVHNA 250 260 270 280 290 300 AAPCCRLVDH PPVNGHRPSV DVLFDSVAEL AGRNAIGVIL TGMGRDGAAG LLKLRHAGAR 310 320 330 340 TFGQNEKTCV VYGMPRVAYE LGAVETQLPL GSIGEEILKT AAARKEGIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a chemotaxis operon in Rhizobium meliloti." Greck M., Platzer J., Sourjik V., Schmitt R. Mol. Microbiol. 15:989-1000(1995) [PubMed: 7623670] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RU11/001.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13166 Genomic DNA. Translation: AAA86677.1. AL591688 Genomic DNA. Translation: CAC45214.1.
PIR	S61837.
RefSeq	NP_384748.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q52883.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1232281.
GenomeReviews	Gene locus R00642 in contig AL591688_GR.
KEGG	sme:SMc03010.
NMPDR	fig|266834.1.peg.1936.
PATRIC	23630499. VBISinMel96828_2015.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705324.
OMA	CAGANTT.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03010-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_RHIME

Accession

Primary (citable) accession number: Q52883

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 2, 2001
Last modified:	January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program