Q52880 (CHEA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis protein CheA
EC=2.7.13.3

Gene names

Name:	cheA
Ordered Locus Names:	R00639
ORF Names:	SMc03007

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either CheB or CheY By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Contains 1 cheW-like domain. Contains 1 histidine kinase domain. Contains 1 HPt domain.

Ontologies

Keywords
Biological process	Chemotaxis Two-component regulatory system
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	Chemotaxis protein CheA		PRO_0000074716
Regions
	Domain	1 – 103	103	HPt
	Domain	367 – 607	241	Histidine kinase
	Domain	609 – 745	137	CheW-like
Amino acid modifications
	Modified residue	46	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	189	1	D → E in AAA86674. Ref.1
	Sequence conflict	192	1	S → A in AAA86674. Ref.1
	Sequence conflict	215	1	N → D in AAA86674. Ref.1
	Sequence conflict	297	1	E → K in AAA86674. Ref.1
	Sequence conflict	308	1	S → P in AAA86674. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q52880 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 9F03A8F24EA25D5D	FASTA	758	81,102
	10 20 30 40 50 60 MDMNEIKEIF FQECEEQLAE LESGLLKLND GDRDPETVNA VFRAVHSIKG GAGAFGLDDL 70 80 90 100 110 120 VSFAHVFETT LDCVRSNRLE PNQDVLKVML RSADVLADLT NAARDGGGVD EARSRQLIKE 130 140 150 160 170 180 LEALANGELP QAAAESAPKT TPAGVAPAAP VVNEEGFQPV AFSFDDFETG DEPTIEPSTY 190 200 210 220 230 240 EIVFKPKSDL YSKGNDATLL LRDLSRLGEM SIHCNMDTLP PLDRMNPEEA YFSWKISLKT 250 260 270 280 290 300 DKGEEAIRSV FEFAEWDCEL DVALAGGTVG MDEDLPMQPV PFDLSILEDE AQAPAGEEDR 310 320 330 340 350 360 AAASEGDSRN AAVAAAQTAS NVLQMAQSTA RVSPENARNS QSASAAQAAA QQAASAATPT 370 380 390 400 410 420 IRVDLDRVDR LINLVGELVI NQAMLSQSVI ENDTNGTSSI NMGLEELQQL TREIQDSVMA 430 440 450 460 470 480 IRAQPVKPVF QRMSRIVREI ADMTGKSVRL ITEGENTEVD KTVIDKLAEP LTHMIRNAVD 490 500 510 520 530 540 HGLETPEKRV AAGKNPEGTV RLTAKHRSGR IVIELADDGA GINREKVRQK AIDNDLIAAD 550 560 570 580 590 600 ANLSDEEVDN LIFHAGFSTA DKISDISGRG VGMDVVKRSI QALGGRINIS SKPGQGSIFT 610 620 630 640 650 660 MSLPLTLAVL DGMVVTVANQ TLVVPLTAIV ETLQPEASAI HSFGSSQRLI SIRDSFCPLV 670 680 690 700 710 720 DVGRILNFRG AQANPVEGVA LLVESEGGGQ RALMVDAIQG QRQVVIKSLE ANYTHVPGIA 730 740 750 AATILGDGRV ALILDVDAIV AASRGQSLKP EMSLAAAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a chemotaxis operon in Rhizobium meliloti." Greck M., Platzer J., Sourjik V., Schmitt R. Mol. Microbiol. 15:989-1000(1995) [PubMed: 7623670] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RU11/001.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13166 Genomic DNA. Translation: AAA86674.1. AL591688 Genomic DNA. Translation: CAC45211.1.
PIR	S61834.
RefSeq	NP_384745.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q52880.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1232278.
GenomeReviews	Gene locus R00639 in contig AL591688_GR.
KEGG	sme:SMc03007.
NMPDR	fig|266834.1.peg.1933.
PATRIC	23630493. VBISinMel96828_2012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705053.
OMA	KSTAHEH.
ProtClustDB	CLSK886270.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03007-MONOMER.
BRENDA	2.7.13.3. 5347.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR002545. CheW. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_Hpt_dom. IPR004105. Sig_transdc_His_kin_subgr_dim. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:1.20.120.160. Sig_transdc_His_kin_Hpt_dom. 1 hit. G3DSA:1.10.287.560. Sig_transdc_His_kin_subgr_dim. 1 hit.
KO	K03407.
Pfam	PF01584. CheW. 1 hit. PF02895. H-kinase_dim. 1 hit. PF02518. HATPase_c. 1 hit. PF01627. Hpt. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00260. CheW. 1 hit. SM00387. HATPase_c. 1 hit. SM00073. HPT. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF50341. CheW. 1 hit. SSF47384. His_kin_homodim. 1 hit. SSF47226. Hpt. 1 hit.
PROSITE	PS50851. CHEW. 1 hit. PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEA_RHIME

Accession

Primary (citable) accession number: Q52880

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 2, 2001
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program