Reviewed, UniProtKB/Swiss-Prot Q52811 (METC_RHIL3)

Names and origin

Protein names

Recommended name:
    Putative cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase
    ORF5

Gene names

Name:	metC
Ordered Locus Names:	RL2205

Organism

Rhizobium leguminosarum bv. viciae (strain 3841) [Complete proteome] [HAMAP]

Taxonomic identifier

216596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Putative cystathionine beta-lyase		PRO_0000114771
Amino acid modifications
	Modified residue	210	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q52811-1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 33C516CF75DD23B8	FASTA	396	43,004
	10 20 30 40 50 60 MKDKDSLLQN AGINTRLTHI GNDPFDYHGF INPPVVHAST VLFPNARAME TRTQKYTYGT 70 80 90 100 110 120 RGTPTTDALC EAIDALEGSA GTILVPSGLA AVTIPFLGFV AAGDHALVVD SVYGPTRHFC 130 140 150 160 170 180 DTMLKRLGVE VEYYHPEIGA GIETLFRSNT KLVHTEAPGS NTFEMQDIPA ISAVAHRHGA 190 200 210 220 230 240 VVMMDNTWAT PVYFRPLDHG VDISIHASTK YPSGHSDILL GTVSANAEHW ERLKEANGVL 250 260 270 280 290 300 GICGAPDDAY QILRGLRTMG LRLERHYESA LDIAKWLEGR DDVARVLHPA LPSFPSHHLW 310 320 330 340 350 360 KRDFKGASGI FSFVLAADGP EKSRAKAHAF LDALRIFGLG YSWGGFESLA LHAYLNDRTV 370 380 390 AKAPTDGPVI RLQIGIEDVA DLKADIERGF AAASAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of Rhizobium leguminosarum has recognizable core and accessory components." Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F., Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H., East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I., Chillingworth T., Clarke K. , Cronin A., Davis P., Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J. Genome Biol. 7:RESEARCH34.1-RESEARCH34.20(2006) [PubMed: 16640791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The general L-amino acid permease of Rhizobium leguminosarum is an ABC uptake system that also influences efflux of solutes." Walshaw D.L., Poole P.S. Mol. Microbiol. 21:1239-1252(1996) [PubMed: 8898392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM236080 Genomic DNA. Translation: CAK07697.1. X82596 Genomic DNA. Translation: CAA57932.1.
RefSeq	YP_767799.1.
3D structure databases
HSSP	HSSP built from PDB template 1CL2 based on UniProtKB P06721.
ModBase	Search...
Protein-protein interaction databases
STRING	Q52811.
Genome annotation databases
GeneID	4402983.
GenomeReviews	Gene locus RL2205 in contig AM236080_GR.
KEGG	rle:RL2205.
NMPDR	fig|216596.1.peg.2287.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q52811.
OMA	IETTYYD.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006233. Cys_b_lyase_bac. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808:SF8. Cys_b_lyase_bac. 1 hit. PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
TIGRFAMs	TIGR01324. cysta_beta_ly_B. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METC_RHIL3

Accession

Primary (citable) accession number: Q52811
Secondary accession number(s): Q1MH70

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 27, 2006
Last modified:	November 3, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)