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Q52698

- RNC_RHOCA

UniProt

Q52698 - RNC_RHOCA

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Protein

Ribonuclease 3

Gene

rnc

Organism
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs. Complements an E.coli disruption mutant, but the E.coli enzyme does not cleave R.capsulatus rRNA precursor, showing substrate recognition is different. Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.3 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+1 Publication

pH dependencei

Optimum pH is 7.5 at 32 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451MagnesiumBy similarity
Active sitei49 – 491Sequence Analysis
Metal bindingi118 – 1181MagnesiumBy similarity
Active sitei121 – 1211By similarity
Metal bindingi121 – 1211MagnesiumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
OrganismiRhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifieri1061 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of processing of 23S rRNA. Full complementation requires both lep and rnc genes, suggesting they form an operon.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Ribonuclease 3PRO_0000180427Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ52698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 132126RNase IIIAdd
BLAST
Domaini157 – 22670DRBMAdd
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.Curated

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q52698-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVAADLSAF MDRLGHRFTT PEHLVRALTH SSLGSATRPD NQRLEFLGDR
60 70 80 90 100
VLGLSMAEAL FHADGRASEG QLAPRFNALV RKETCAAVAR DIDLGAVLKL
110 120 130 140 150
GRSEMMSGGR RKDALLGDAM EAVIAAVYLD AGFEVARALV LRLWAARIQS
160 170 180 190 200
VDNDARDPKT ALQEWAQARG LPPPRYETLG RDGPDHAPQF RIAVVLASGE
210 220
TEEAQAGSKR NAEQAAAKAL LERLERGA
Length:228
Mass (Da):24,663
Last modified:November 1, 1996 - v1
Checksum:i8B4AEFF3434E699C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti62 – 621H → D in strain: DSM 938.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68305 Genomic DNA. Translation: CAA92647.1.
PIRiS66596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68305 Genomic DNA. Translation: CAA92647.1 .
PIRi S66596.

3D structure databases

ProteinModelPortali Q52698.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification and analysis of the rnc gene for RNase III in Rhodobacter capsulatus."
    Rauhut R., Jaeger A., Conrad C., Klug G.
    Nucleic Acids Res. 24:1246-1251(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN RRNA PROCESSING, EXPRESSION IN E.COLI, DISRUPTION PHENOTYPE.
    Strain: ATCC 33303 / B10 and DSM 938 / 37b4.
  2. "Cloning of a gene involved in rRNA precursor processing and 23S rRNA cleavage in Rhodobacter capsulatus."
    Kordes E., Jock S., Fritsch J., Bosch F., Klug G.
    J. Bacteriol. 176:1121-1127(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: DSM 938 / 37b4.
  3. "Different cleavage specificities of RNases III from Rhodobacter capsulatus and Escherichia coli."
    Conrad C., Rauhut R., Klug G.
    Nucleic Acids Res. 26:4446-4453(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, FUNCTION IN RRNA PROCESSING, RRNA-BINDING, SUBSTRATE SPECIFICITY.
    Strain: ATCC 33303 / B10.

Entry informationi

Entry nameiRNC_RHOCA
AccessioniPrimary (citable) accession number: Q52698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The protein in strain DMS 938 / 37b4 has 5 other sequence differences. In Rhodobacter species, 23S rRNA is further processed to 16S and 14S rRNA species in vivo, probably by RNase III.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3