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Q52698

- RNC_RHOCA

UniProt

Q52698 - RNC_RHOCA

Protein

Ribonuclease 3

Gene

rnc

Organism
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs. Complements an E.coli disruption mutant, but the E.coli enzyme does not cleave R.capsulatus rRNA precursor, showing substrate recognition is different. Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.3 Publications

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Mg2+.1 Publication

    pH dependencei

    Optimum pH is 7.5 at 32 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi45 – 451MagnesiumBy similarity
    Active sitei49 – 491Sequence Analysis
    Metal bindingi118 – 1181MagnesiumBy similarity
    Active sitei121 – 1211By similarity
    Metal bindingi121 – 1211MagnesiumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB-HAMAP
    3. rRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-HAMAP
    2. rRNA catabolic process Source: InterPro
    3. rRNA processing Source: UniProtKB-HAMAP
    4. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, rRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3 (EC:3.1.26.3)
    Alternative name(s):
    Ribonuclease III
    Short name:
    RNase III
    Gene namesi
    Name:rnc
    OrganismiRhodobacter capsulatus (Rhodopseudomonas capsulata)
    Taxonomic identifieri1061 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Loss of processing of 23S rRNA. Full complementation requires both lep and rnc genes, suggesting they form an operon.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228Ribonuclease 3PRO_0000180427Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ52698.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 132126RNase IIIAdd
    BLAST
    Domaini157 – 22670DRBMAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RNase III domain.Curated

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q52698-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVAADLSAF MDRLGHRFTT PEHLVRALTH SSLGSATRPD NQRLEFLGDR    50
    VLGLSMAEAL FHADGRASEG QLAPRFNALV RKETCAAVAR DIDLGAVLKL 100
    GRSEMMSGGR RKDALLGDAM EAVIAAVYLD AGFEVARALV LRLWAARIQS 150
    VDNDARDPKT ALQEWAQARG LPPPRYETLG RDGPDHAPQF RIAVVLASGE 200
    TEEAQAGSKR NAEQAAAKAL LERLERGA 228
    Length:228
    Mass (Da):24,663
    Last modified:November 1, 1996 - v1
    Checksum:i8B4AEFF3434E699C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti62 – 621H → D in strain: DSM 938.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68305 Genomic DNA. Translation: CAA92647.1.
    PIRiS66596.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68305 Genomic DNA. Translation: CAA92647.1 .
    PIRi S66596.

    3D structure databases

    ProteinModelPortali Q52698.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and analysis of the rnc gene for RNase III in Rhodobacter capsulatus."
      Rauhut R., Jaeger A., Conrad C., Klug G.
      Nucleic Acids Res. 24:1246-1251(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN RRNA PROCESSING, EXPRESSION IN E.COLI, DISRUPTION PHENOTYPE.
      Strain: ATCC 33303 / B10 and DSM 938 / 37b4.
    2. "Cloning of a gene involved in rRNA precursor processing and 23S rRNA cleavage in Rhodobacter capsulatus."
      Kordes E., Jock S., Fritsch J., Bosch F., Klug G.
      J. Bacteriol. 176:1121-1127(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: DSM 938 / 37b4.
    3. "Different cleavage specificities of RNases III from Rhodobacter capsulatus and Escherichia coli."
      Conrad C., Rauhut R., Klug G.
      Nucleic Acids Res. 26:4446-4453(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, FUNCTION IN RRNA PROCESSING, RRNA-BINDING, SUBSTRATE SPECIFICITY.
      Strain: ATCC 33303 / B10.

    Entry informationi

    Entry nameiRNC_RHOCA
    AccessioniPrimary (citable) accession number: Q52698
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The protein in strain DMS 938 / 37b4 has 5 other sequence differences. In Rhodobacter species, 23S rRNA is further processed to 16S and 14S rRNA species in vivo, probably by RNase III.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3