Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase

Gene

mhpC

Organism
Pseudomonas sp.
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6-oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid.UniRule annotation

Catalytic activityi

(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate.UniRule annotation
(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate.UniRule annotation

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111Transition state stabilizerUniRule annotation1
Sitei189Catalytic role in ketonization of the dienol substrate (substrate destabilization)UniRule annotation1
Active sitei263Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

UniPathwayiUPA00714.

Protein family/group databases

ESTHERipsesp-pcbD. Carbon-carbon_bond_hydrolase.
MEROPSiS33.016.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolaseUniRule annotation (EC:3.7.1.14UniRule annotation)
Alternative name(s):
2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolaseUniRule annotation
2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolaseUniRule annotation
2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolaseUniRule annotation
Gene namesi
Name:mhpCUniRule annotation
Synonyms:pcbD
OrganismiPseudomonas sp.
Taxonomic identifieri306 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003377861 – 2832-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolaseAdd BLAST283

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ52532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 269AB hydrolase-1Sequence analysisAdd BLAST235

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. MhpC family.Curated

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01654. MhpC. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR023791. MhpC_alpha/beta_hydrolase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q52532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAITEAGSS KFIDVTEGEI NGSIHVNDAG NGDEVVVMFH GSGPGASGWS
60 70 80 90 100
NFHRNVDAFV DAGYRVLLID SPGFNKSYPI VTKSRDGAYA QAAKGVMDKL
110 120 130 140 150
GIKRAHMIGN SMGGATAMRM AVDYPEMVGK LVMMGGGSVG GSTTTPMPTE
160 170 180 190 200
GLKLLQGLYR NPSMENLRKM LDIFVYAPST LTEELINGRF ENMMRRPEHL
210 220 230 240 250
TNFVESLKAS GGRANYAHLL PTLTMPTMII WGRDDRFVPL DLGLRMLWGM
260 270 280
PDAELHVFSK CGHWAQWEHA DKFNQLVLNF LAR
Length:283
Mass (Da):31,032
Last modified:November 1, 1996 - v1
Checksum:i6C493751B1E8C4CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D44550 Genomic DNA. Translation: BAA07955.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D44550 Genomic DNA. Translation: BAA07955.1.

3D structure databases

ProteinModelPortaliQ52532.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERipsesp-pcbD. Carbon-carbon_bond_hydrolase.
MEROPSiS33.016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00714.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01654. MhpC. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR023791. MhpC_alpha/beta_hydrolase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMHPC_PSESP
AccessioniPrimary (citable) accession number: Q52532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.