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Protein

Alpha-amylase

Gene
N/A
Organism
Pseudomonas sp.
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
OrganismiPseudomonas sp.Imported
Taxonomic identifieri306 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 614588Alpha-amylaseSequence analysisPRO_5004248450Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ52516.
SMRiQ52516. Positions 31-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini509 – 614106CBM20 (carbohydrate binding type-20)InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotationSAAS annotation

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF00686. CBM_20. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q52516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRLALALA ASAVLAGPWA VGAVQAQSAP RTAFVQLFEW KWTDVARECE
60 70 80 90 100
TYLGPKGFAA VQISPPNEHN WVSSGDGAPY PWWMRYQPVS YSLDRSRSGT
110 120 130 140 150
RAEFQDMVNR CNAAGVGIYV DAVINHMSGG NGGTSSAGRS WSHHNYPGLY
160 170 180 190 200
GSQDFHHPVC AITNYGDANN VQNCELSGLQ DLNTGSSYVR GKISDYLVDL
210 220 230 240 250
VQMGVKGLRV DAAKHISPTD LGAIIDSVNA RTGAARPFWF LEVIGAPGEA
260 270 280 290 300
VQPSQYFGLG GGQVTVTEFA YGKELYGKFA GGGKLADLQT FGPSWNLMPS
310 320 330 340 350
SKAIAFVDNH DKQRGHGGGG GYVTYHHGST YDLANIFMLA WPYGYPALMS
360 370 380 390 400
ATASTRAAAT TPATARRTTA TARPGGRGTG TPPARVLQPD RGWLGLRAPL
410 420 430 440 450
ARHRQHGGLP QRHRGQLVRQ RLVEQRQQPD RLRPRRQGLR RHQQGRYGAD
460 470 480 490 500
AASRPACRPG VLRRDLRRLR QRQLHRHGGD RGCRRPRDAV RTRLWRGGDP
510 520 530 540 550
RRCAHRRHAA GAAVLSLTFN ETADTVWGQN LFVVGNVGAL GNWAPAAGAA
560 570 580 590 600
MTWISGSGST GQWRATVQLP ADTPVQYKYV KKDGAGNVVW ESGGNRVVTT
610
PAPGATIAVN DSWK
Length:614
Mass (Da):66,541
Last modified:November 1, 1996 - v1
Checksum:i1DE220E15B271EB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10769 Genomic DNA. Translation: BAA01600.1.
PIRiJS0631.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10769 Genomic DNA. Translation: BAA01600.1.
PIRiJS0631.

3D structure databases

ProteinModelPortaliQ52516.
SMRiQ52516. Positions 31-352.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006046. Alpha_amylase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF00686. CBM_20. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and nucleotide sequence of the maltopentaose-forming amylase gene from Pseudomonas sp. KO-8940."
    Shida O., Takano T., Takagi H., Kadowaki K., Kobayashi S.
    Biosci. Biotechnol. Biochem. 56:76-80(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KO-8940Imported.
  2. "Cloning and nucleotide sequence of the maltopentaose forming amylase gene from Pseudomonas sp. KO-8940."
    Shida O., Takano T., Takagi H., Kadowaki K., Kobayashi S.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KO-8940Imported.

Entry informationi

Entry nameiQ52516_PSESP
AccessioniPrimary (citable) accession number: Q52516
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.