Q52424 (AAC2_PROST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminoglycoside 2'-N-acetyltransferase
EC=2.3.1.59

Alternative name(s):
AAC(2')-Ia

Gene names

Name:

aac

Organism

Providencia stuartii

Taxonomic identifier

588 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Providencia

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the O-acetylation of peptidoglycan. It is also capable of acetylating and inactivating certain aminoglycosides and confers high-level resistance to these antibiotics when overexpressed.
Catalytic activity	Acetyl-CoA + gentamicin C(1a) = CoA + N(2')-acetylgentamicin C(1a).
Sequence similarities	Belongs to the AAC(2')-I acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminoglycoside 2'-N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Aminoglycoside 2'-N-acetyltransferase		PRO_0000064413
Regions
	Domain	8 – 171	164	N-acetyltransferase
	Region	79 – 80	2	Substrate binding By similarity
	Region	81 – 83	3	Coenzyme A binding By similarity
	Region	88 – 93	6	Coenzyme A binding By similarity
	Region	148 – 149	2	Substrate binding By similarity
Sites
	Binding site	32	1	Substrate By similarity
	Binding site	114	1	Substrate; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q52424 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A7D72B79AF6A9AC9	FASTA	178	20,087
	10 20 30 40 50 60 MGIEYRSLHT SQLTLSEKEA LYDLLIEGFE GDFSHDDFAH TLGGMHVMAF DQQKLVGHVA 70 80 90 100 110 120 IIQRHMALDN TPISVGYVEA MVVEQSYRRQ GIGRQLMLQT NKIIASCYQL GLLSASDDGQ 130 140 150 160 170 KLYHSVGWQI WKGKLFELKQ GSYIRSIEEE GGVMGWKADG EVDFTASLYC DFRGGDQW

References

[1]	"Characterization and transcriptional regulation of the 2'-N-acetyltransferase gene from Providencia stuartii." Rather P.N., Orosz E., Shaw K.J., Hare R., Miller G. J. Bacteriol. 175:6492-6498(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SCH75082831A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06156 Unassigned RNA. Translation: AAA03550.1.
PIR	A49899.
3D structure databases
ProteinModelPortal	Q52424.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAC2_PROST

Accession

Primary (citable) accession number: Q52424

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program