Q52186 (POBB_PSEPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenoxybenzoate dioxygenase subunit beta
EC=1.-.-.-

Alternative name(s):
4-carboxydiphenyl ether;phenoxybenzoate dioxygenase subunit beta

Gene names

Name:

pobB

Encoded on

Plasmid pPOB

Organism

Pseudomonas pseudoalcaligenes

Taxonomic identifier

330 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas oleovorans/pseudoalcaligenes group

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate.
Cofactor	FMN By similarity.
Pathway	Aromatic compound metabolism; carboxydiphenyl ether degradation.
Subunit structure	This dioxygenase system consists of two proteins: the alpha subunit (PobA) and a subunit (PobB) that acts as a ferredoxin and a ferredoxin reductase.
Induction	By 3- or 4-carboxydiphenyl ether and by phenol.
Sequence similarities	Belongs to the PDR/VanB family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Electron transport Transport
Ligand	2Fe-2S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Phenoxybenzoate dioxygenase subunit beta		PRO_0000189399
Regions
	Domain	7 – 109	103	FAD-binding FR-type
	Domain	234 – 319	86	2Fe-2S ferredoxin-type
	Nucleotide binding	113 – 223	111	NAD By similarity
Sites
	Metal binding	268	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	273	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	276	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	306	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q52186 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 14CDB6B17D6CF606	FASTA	319	33,591
	10 20 30 40 50 60 MSAAATMAPV SLRIHAIAYG ADDVLLFDLR APARDGLAPF DAGAHIDLRL PRGITRSYSL 70 80 90 100 110 120 LNDPAERHRY VIGVKREPES RGGSAWLHAD ARVGALIEVD GPSNHFALDE SAPHAVFIAG 130 140 150 160 170 180 GIGITPLWSM VQRLEHLGTP WTLHYRARSR RGAALLDELA GHGDRVHLSF SDEGAPSLDL 190 200 210 220 230 240 AAIVAAAPEG AHFYCCGPVP MLEAFEAACV GLDPARVHLE YFAAKEAPAT EGGFVVHLAR 250 260 270 280 290 300 SGRTIPIAAG CTILDALQAG GVAVPSSCQQ GVCGICETAV LAGVPDHRDL VLSDQERAAG 310 RTMMICCSGS KTAELTLDL

References

[1]

"Cloning, nucleotide sequence, and expression of the gene encoding a novel dioxygenase involved in metabolism of carboxydiphenyl ethers in Pseudomonas pseudoalcaligenes POB310." Dehmel U., Engesser K.-H., Timmis K.N., Dwyer D.F. Arch. Microbiol. 163:35-41(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: POB310.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78823 Genomic DNA. Translation: CAA55401.1.
PIR	S44172.
3D structure databases
ProteinModelPortal	Q52186.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00730.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR006058. 2Fe2S_fd_BS. IPR012675. Beta-grasp_dom. IPR017927. Fd_Rdtase_FAD-bd. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR000951. Ph_dOase_redase. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00409. PHDIOXRDTASE.
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POBB_PSEPS

Accession

Primary (citable) accession number: Q52186

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program