Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q52060

- ACDH_PSEUF

UniProt

Q52060 - ACDH_PSEUF

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Acetaldehyde dehydrogenase

Gene

dmpF

Organism
Pseudomonas sp. (strain CF600)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Can also act on propanal and butanal to form propanoyl-CoA and butanoyl-CoA, respectively. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds such as phenols, cresols and catechols. NADP+ can replace NAD+ but the rate of reaction is much slower.1 Publication

Catalytic activityi

Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.1 Publication

Enzyme regulationi

Is not activated by Mn2+, Mg2+, Ca2+, Zn2+ or Co2+.1 Publication

pH dependencei

Activity increases gradually over the pH range 6.5-8.5.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei132 – 1321Acyl-thioester intermediateBy similarity
Binding sitei290 – 2901NAD1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 154NAD1 Publication
Nucleotide bindingi163 – 1719NAD1 Publication

GO - Molecular functioni

  1. acetaldehyde dehydrogenase (acetylating) activity Source: UniProtKB
  2. NAD binding Source: UniProtKB
  3. NADP binding Source: UniProtKB

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB
  2. phenol-containing compound catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12780.
UniPathwayiUPA00728.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetaldehyde dehydrogenase (EC:1.2.1.10)
Alternative name(s):
Acetaldehyde dehydrogenase [acetylating]
Gene namesi
Name:dmpF
Encoded oniPlasmid pVI1500 Publication
OrganismiPseudomonas sp. (strain CF600)
Taxonomic identifieri79676 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Acetaldehyde dehydrogenasePRO_0000404201Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of two DmpG (aldolase) and two DmpF (dehydrogenase) subunits, which allows a direct channeling of acetaldehyde between the two active sites.2 Publications

Protein-protein interaction databases

IntActiQ52060. 1 interaction.

Structurei

Secondary structure

1
312
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106
Helixi14 – 2613
Beta strandi28 – 369
Helixi43 – 508
Beta strandi55 – 584
Helixi59 – 657
Helixi67 – 715
Beta strandi72 – 776
Helixi81 – 9414
Beta strandi99 – 1024
Helixi114 – 1174
Turni118 – 1236
Beta strandi125 – 1284
Helixi132 – 14514
Beta strandi150 – 16011
Helixi161 – 1633
Helixi166 – 1694
Helixi172 – 18514
Beta strandi190 – 19910
Beta strandi207 – 21711
Helixi220 – 23516
Beta strandi241 – 2455
Beta strandi248 – 2525
Beta strandi258 – 2603
Turni261 – 2633
Beta strandi264 – 2663
Beta strandi268 – 27710
Beta strandi282 – 2843
Helixi289 – 31022

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NVMX-ray1.70B/D/F/H1-312[»]
ProteinModelPortaliQ52060.
SMRiQ52060. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ52060.

Family & Domainsi

Sequence similaritiesi

Belongs to the acetaldehyde dehydrogenase family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01657. Ac_ald_DH_ac.
InterProiIPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03215. ac_ald_DH_ac. 1 hit.

Sequencei

Sequence statusi: Complete.

Q52060-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQKLKVAII GSGNIGTDLM IKVLRNAKYL EMGAMVGIDA ASDGLARAQR
60 70 80 90 100
MGVTTTYAGV EGLIKLPEFA DIDFVFDATS ASAHVQNEAL LRQAKPGIRL
110 120 130 140 150
IDLTPAAIGP YCVPVVNLEE HLGKLNVNMV TCGGQATIPM VAAVSRVAKV
160 170 180 190 200
HYAEIVASIS SKSAGPGTRA NIDEFTETTS KAIEVIGGAA KGKAIIIMNP
210 220 230 240 250
AEPPLIMRDT VYVLSAAADQ AAVAASVAEM VQAVQAYVPG YRLKQQVQFD
260 270 280 290 300
VIPESAPLNI PGLGRFSGLK TSVFLEVEGA AHYLPAYAGN LDIMTSAALA
310
TAERMAQSML NA
Length:312
Mass (Da):32,685
Last modified:November 1, 1996 - v1
Checksum:i2B38A98CE97B50DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60835 Genomic DNA. Translation: CAA43226.1.
PIRiS24419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60835 Genomic DNA. Translation: CAA43226.1 .
PIRi S24419.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NVM X-ray 1.70 B/D/F/H 1-312 [» ]
ProteinModelPortali Q52060.
SMRi Q52060. Positions 1-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q52060. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00728 .
BioCyci MetaCyc:MONOMER-12780.

Miscellaneous databases

EvolutionaryTracei Q52060.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_01657. Ac_ald_DH_ac.
InterProi IPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view ]
Pfami PF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTi SM00859. Semialdhyde_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600."
    Shingler V., Marklund U., Powlowski J.
    J. Bacteriol. 174:711-724(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: CF600.
  2. "Purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600."
    Powlowski J., Sahlman L., Shingler V.
    J. Bacteriol. 175:377-385(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-6, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, ENZYME REGULATION, SUBUNIT, INTERACTION WITH DMPG, PH DEPENDENCE.
    Strain: CF600.
  3. "Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate."
    Manjasetty B.A., Powlowski J., Vrielink A.
    Proc. Natl. Acad. Sci. U.S.A. 100:6992-6997(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH DMPG AND NAD, SUBUNIT, REACTION MECHANISM.
    Strain: CF600.

Entry informationi

Entry nameiACDH_PSEUF
AccessioniPrimary (citable) accession number: Q52060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3