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Protein

Acetaldehyde dehydrogenase

Gene

dmpF

Organism
Pseudomonas sp. (strain CF600)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of acetaldehyde to acetyl-CoA, using NAD+ and coenzyme A. Can also act on propanal and butanal to form propanoyl-CoA and butanoyl-CoA, respectively. Is the final enzyme in the meta-cleavage pathway for the degradation of aromatic compounds such as phenols, cresols and catechols. NADP+ can replace NAD+ but the rate of reaction is much slower.1 Publication

Catalytic activityi

Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.1 Publication

Enzyme regulationi

Is not activated by Mn2+, Mg2+, Ca2+, Zn2+ or Co2+.1 Publication

pH dependencei

Activity increases gradually over the pH range 6.5-8.5.1 Publication

Pathwayi: phenol degradation

This protein is involved in the pathway phenol degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenol degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei132Acyl-thioester intermediateBy similarity1
Binding sitei290NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15NAD1 Publication4
Nucleotide bindingi163 – 171NAD1 Publication9

GO - Molecular functioni

  • acetaldehyde dehydrogenase (acetylating) activity Source: UniProtKB
  • NAD binding Source: UniProtKB
  • NADP binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12780.
BRENDAi1.2.1.10. 5085.
UniPathwayiUPA00728.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetaldehyde dehydrogenase (EC:1.2.1.10)
Alternative name(s):
Acetaldehyde dehydrogenase [acetylating]
Gene namesi
Name:dmpF
Encoded oniPlasmid pVI1500 Publication
OrganismiPseudomonas sp. (strain CF600)
Taxonomic identifieri79676 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004042011 – 312Acetaldehyde dehydrogenaseAdd BLAST312

Interactioni

Subunit structurei

Heterotetramer composed of two DmpG (aldolase) and two DmpF (dehydrogenase) subunits, which allows a direct channeling of acetaldehyde between the two active sites.2 Publications

Protein-protein interaction databases

IntActiQ52060. 1 interactor.

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi14 – 26Combined sources13
Beta strandi28 – 36Combined sources9
Helixi43 – 50Combined sources8
Beta strandi55 – 58Combined sources4
Helixi59 – 65Combined sources7
Helixi67 – 71Combined sources5
Beta strandi72 – 77Combined sources6
Helixi81 – 94Combined sources14
Beta strandi99 – 102Combined sources4
Helixi114 – 117Combined sources4
Turni118 – 123Combined sources6
Beta strandi125 – 128Combined sources4
Helixi132 – 145Combined sources14
Beta strandi150 – 160Combined sources11
Helixi161 – 163Combined sources3
Helixi166 – 169Combined sources4
Helixi172 – 185Combined sources14
Beta strandi190 – 199Combined sources10
Beta strandi207 – 217Combined sources11
Helixi220 – 235Combined sources16
Beta strandi241 – 245Combined sources5
Beta strandi248 – 252Combined sources5
Beta strandi258 – 260Combined sources3
Turni261 – 263Combined sources3
Beta strandi264 – 266Combined sources3
Beta strandi268 – 277Combined sources10
Beta strandi282 – 284Combined sources3
Helixi289 – 310Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NVMX-ray1.70B/D/F/H1-312[»]
ProteinModelPortaliQ52060.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ52060.

Family & Domainsi

Sequence similaritiesi

Belongs to the acetaldehyde dehydrogenase family.Curated

Phylogenomic databases

KOiK04073.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01657. Ac_ald_DH_ac. 1 hit.
InterProiIPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03215. ac_ald_DH_ac. 1 hit.

Sequencei

Sequence statusi: Complete.

Q52060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQKLKVAII GSGNIGTDLM IKVLRNAKYL EMGAMVGIDA ASDGLARAQR
60 70 80 90 100
MGVTTTYAGV EGLIKLPEFA DIDFVFDATS ASAHVQNEAL LRQAKPGIRL
110 120 130 140 150
IDLTPAAIGP YCVPVVNLEE HLGKLNVNMV TCGGQATIPM VAAVSRVAKV
160 170 180 190 200
HYAEIVASIS SKSAGPGTRA NIDEFTETTS KAIEVIGGAA KGKAIIIMNP
210 220 230 240 250
AEPPLIMRDT VYVLSAAADQ AAVAASVAEM VQAVQAYVPG YRLKQQVQFD
260 270 280 290 300
VIPESAPLNI PGLGRFSGLK TSVFLEVEGA AHYLPAYAGN LDIMTSAALA
310
TAERMAQSML NA
Length:312
Mass (Da):32,685
Last modified:November 1, 1996 - v1
Checksum:i2B38A98CE97B50DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60835 Genomic DNA. Translation: CAA43226.1.
PIRiS24419.

Genome annotation databases

KEGGiag:CAA43226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60835 Genomic DNA. Translation: CAA43226.1.
PIRiS24419.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NVMX-ray1.70B/D/F/H1-312[»]
ProteinModelPortaliQ52060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ52060. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA43226.

Phylogenomic databases

KOiK04073.

Enzyme and pathway databases

UniPathwayiUPA00728.
BioCyciMetaCyc:MONOMER-12780.
BRENDAi1.2.1.10. 5085.

Miscellaneous databases

EvolutionaryTraceiQ52060.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01657. Ac_ald_DH_ac. 1 hit.
InterProiIPR003361. Acetaldehyde_dehydrogenase.
IPR015426. Acetylaldehyde_DH_C.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF09290. AcetDehyd-dimer. 1 hit.
PF01118. Semialdhyde_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015689. Actaldh_dh_actl. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03215. ac_ald_DH_ac. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACDH_PSEUF
AccessioniPrimary (citable) accession number: Q52060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.