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Protein

2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase

Gene

bphD

Organism
Pseudomonas pseudoalcaligenes
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).1 Publication

Catalytic activityi

2,6-dioxo-6-phenylhexa-3-enoate + H2O = benzoate + 2-oxopent-4-enoate.

Pathwayi: biphenyl degradation

This protein is involved in step 4 of the subpathway that synthesizes 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Biphenyl dioxygenase subunit alpha (bphA)
  2. Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (bphB)
  3. Biphenyl-2,3-diol 1,2-dioxygenase (bphC)
  4. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD)
This subpathway is part of the pathway biphenyl degradation, which is itself part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl, the pathway biphenyl degradation and in Xenobiotic degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51SubstrateBy similarity1
Binding sitei111SubstrateBy similarity1
Sitei112Transition state stabilizerBy similarity1
Binding sitei180Substrate; via carbonyl oxygenBy similarity1
Binding sitei190SubstrateBy similarity1
Active sitei265Proton acceptorBy similarity1
Binding sitei266SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAromatic hydrocarbons catabolism

Enzyme and pathway databases

UniPathwayiUPA00155; UER00253.

Protein family/group databases

ESTHERipseps-bphd. Carbon-carbon_bond_hydrolase.
MEROPSiS33.016.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC:3.7.1.8)
Short name:
HOPDA hydrolase
Alternative name(s):
2,6-dioxo-6-phenylhexa-3-enoate hydrolase
Gene namesi
Name:bphD
OrganismiPseudomonas pseudoalcaligenes
Taxonomic identifieri330 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonasPseudomonas oleovorans/pseudoalcaligenes group

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003738141 – 2862-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolaseAdd BLAST286

Expressioni

Inductioni

By biphenyl.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ52011.
SMRiQ52011.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 271AB hydrolase-1Sequence analysisAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 43Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. BphD family.Curated

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01688. Biphenyl_BphD. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR017727. HOPD_hydrolase_BphD.
PfamiView protein in Pfam
PF00561. Abhydrolase_1. 1 hit.
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03343. biphenyl_bphD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q52011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALTESSTS KFVKINEKGF SDFNIHYNEA GNGETVIMLH GGGPGAGGWS
60 70 80 90 100
NYYRNVGPFV DAGYRVILKD SPGFNKSDAV VMDEQRGLVN ARAVKGLMDA
110 120 130 140 150
LGIDRAHLVG NSMGGATALN FAIEYPERIG KLILMGPGGP GPSMFAPMPM
160 170 180 190 200
EGIKLLFKLY AEPSYENLKQ MIQVFLYDQS LITEELLQGR WEAIQRQPEH
210 220 230 240 250
LKNFLISAQK APLSTWDVTA RLGEIKAKTF ITWGRDDRFV PLDHGLKLLW
260 270 280
NIDDARLHVF SKCGHWAQWE HADEFNRLAI DFLRQA
Length:286
Mass (Da):31,946
Last modified:November 1, 1996 - v1
Checksum:iB204AADDC7AD31AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85851 Genomic DNA. Translation: BAA12881.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85851 Genomic DNA. Translation: BAA12881.1.

3D structure databases

ProteinModelPortaliQ52011.
SMRiQ52011.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERipseps-bphd. Carbon-carbon_bond_hydrolase.
MEROPSiS33.016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00155; UER00253.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01688. Biphenyl_BphD. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR017727. HOPD_hydrolase_BphD.
PfamiView protein in Pfam
PF00561. Abhydrolase_1. 1 hit.
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03343. biphenyl_bphD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBPHD_PSEPS
AccessioniPrimary (citable) accession number: Q52011
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1996
Last modified: February 15, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.