Q51P78 (ATG12_MAGO7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like protein ATG12

Alternative name(s):
Autophagy-related protein 12

Gene names

Name:	ATG12
ORF Names:	MGG_00598

Organism

Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) [Complete proteome]

Taxonomic identifier

242507 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe › Magnaporthe oryzae

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy. Conjugation with ATG5 through an ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate functions as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes By similarity.
Subunit structure	Forms a conjugate with ATG5 By similarity.
Subcellular location	Preautophagosomal structure membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ATG12 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Membrane
PTM	Isopeptide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Ubiquitin-like protein ATG12		PRO_0000212481
Amino acid modifications
	Cross-link	181		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-162 in ATG5) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51P78 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 86A3BC56930DE80B	FASTA	181	19,118
	10 20 30 40 50 60 MSSPPTRYTR NPQLRRPSLS TPTPPPPSSS STAPASSSAT PIPDDAPDAD DNDGSPPSPD 70 80 90 100 110 120 LPLTMSASVM LTQLPRDATA ALATAGEFPA DQKVVVRFKP VGGSAPALRK ELCKISAAQR 130 140 150 160 170 180 FEAVVAYLRR TLKVGNGESV FLYINSTFAP ALDEIVGNLH RCFKDSNGQL NVSYSMTPAF G

References

[1]

"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM001235 Genomic DNA. Translation: EHA48856.1.
RefSeq	XP_003718440.1. XM_003718392.1.
3D structure databases
ProteinModelPortal	Q51P78.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	MGG_00598T0; MGG_00598T0; MGG_00598.
GeneID	2674234.
KEGG	mgr:MGG_00598.
Phylogenomic databases
eggNOG	NOG236266.
KO	K08336.
OrthoDB	EOG4B2X6W.
Family and domain databases
InterPro	IPR007242. Atg12. [Graphical view]
PANTHER	PTHR13385. PTHR13385. 1 hit.
Pfam	PF04110. APG12. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG12_MAGO7

Accession

Primary (citable) accession number: Q51P78
Secondary accession number(s): A4REQ6, G4NB95

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 7, 2005
Last modified:	May 29, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program